Boswell, Carl A - (cboswell) |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all, As a finer point to the discussion of stage/microscope heater systems, can folks give me an idea of where to find just a heater and thermostat controller for an existing enclosure? I have searched high and low for a recently mentioned heater for incubating eggs, and have come up empty-handed. Everything I've seen comes as complete units, so components are not mentioned. The presumption is that once the desired tempurature is reached, it can be maintained within ± 0.5 degrees or less. Thanks very much, Carl Carl A. Boswell, Ph.D. Molecular and Cellular Biology University of Arizona 520-954-7053 FAX 520-621-3709 |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Carl, We built one (actually I asked someone else to build it) using a proportional controller (Omega CN370, $225). The actual heater is the element from a hair dryer mounted in 3" PVC sewer pipe lined with kaolin batting. A salvaged 3" computer fan provides the flow. A rectifier was added that can be switched in and out of the power circuit to control how much heat is generated. A thermistor to the PID controller provides feedback to the controller. Its been working for about 4 years now, with light use. Hope this helps. Glen Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 [hidden email] ************************************************************************ ****** The box said "Requires WindowsXP or better", so I bought a Macintosh. ************************************************************************ ****** On Dec 17, 2007, at 10:14 AM, Carl Boswell wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Dear all, > As a finer point to the discussion of stage/microscope heater > systems, can folks give me an idea of where to find just a heater > and thermostat controller for an existing enclosure? I have > searched high and low for a recently mentioned heater for > incubating eggs, and have come up empty-handed. Everything I've > seen comes as complete units, so components are not mentioned. The > presumption is that once the desired tempurature is reached, it can > be maintained within ± 0.5 degrees or less. > > Thanks very much, > Carl > > Carl A. Boswell, Ph.D. > Molecular and Cellular Biology > University of Arizona > 520-954-7053 > FAX 520-621-3709 |
In reply to this post by Boswell, Carl A - (cboswell)
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We have been using WPI one for a year now and happy with it. http://www.wpiinc.com/products/microscopy/airtherms/ Good luck! Xuejun PS: no commercial interests, etc. Xue-jun Sun, Ph.D. Dept. Exp. Oncology Cross Cancer Institute 11560 University Ave, Edmonton Alberta T6G 1Z2 Canada Phone: (780) 432-8898 Fax: (780) 432-8425 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Carl Boswell Sent: Monday, December 17, 2007 11:14 AM To: [hidden email] Subject: enclosure heater Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all, As a finer point to the discussion of stage/microscope heater systems, can folks give me an idea of where to find just a heater and thermostat controller for an existing enclosure? I have searched high and low for a recently mentioned heater for incubating eggs, and have come up empty-handed. Everything I've seen comes as complete units, so components are not mentioned. The presumption is that once the desired tempurature is reached, it can be maintained within ± 0.5 degrees or less. Thanks very much, Carl Carl A. Boswell, Ph.D. Molecular and Cellular Biology University of Arizona 520-954-7053 FAX 520-621-3709 This e-mail and any attachments may contain confidential and privileged information. If you are not the intended recipient, please notify the sender immediately by return e-mail, delete this e-mail and destroy any copies. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. |
In reply to this post by Boswell, Carl A - (cboswell)
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Carl, If a heating controler performs to your specs will depend on the material, shape, volume and architecture of your incubator (and the ambient conditions) maybe that's why its difficult to buy a stand alone solution, because no serious manufacturer would like (and be able) to grant you the thing is working in the end. In case you would provide the above mentioned information and the microscope type, I have no doubt folks at embl Heidelberg (embl-em.de) would provide you with their heating unit, which maintaines temp to +-0.1degC between 16 and 42 degC, provided ambient temp between 19 and 24 degC. Of course +-0.5degC might be achievable with a lower cost solution, though be aware you will observe focus shifts with 0.5degC variations at least with >1.2 NA lenses. Hope that helps. regards, jens --- Dr. Jens Rietdorf Head Microscopy Novartis Research Foundation Friedrich-Miescher-Institute, wro1066.2.16 Maulbeerstr.66, CH-4058 Basel, Switzerland phone +41(61)69-75172 mobil +41 798284737 Email:rietdorf(at)fmi.ch -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Carl Boswell Sent: Montag, 17. Dezember 2007 19:14 To: [hidden email] Subject: enclosure heater Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all, As a finer point to the discussion of stage/microscope heater systems, can folks give me an idea of where to find just a heater and thermostat controller for an existing enclosure? I have searched high and low for a recently mentioned heater for incubating eggs, and have come up empty-handed. Everything I've seen comes as complete units, so components are not mentioned. The presumption is that once the desired tempurature is reached, it can be maintained within ± 0.5 degrees or less. Thanks very much, Carl Carl A. Boswell, Ph.D. Molecular and Cellular Biology University of Arizona 520-954-7053 FAX 520-621-3709 |
In reply to this post by Boswell, Carl A - (cboswell)
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal You could try this reference: Kulesa PM, Kasemeier-Kulesa JC. Construction of a Heated Incubation Chamber around a Microscope Stage for Time-Lapse Imaging. CSH Protocols. 2007; doi:10.1101/pdb.prot4792 Claire |
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