fluorescent stain for glass

[Martin Wessendorf-2]

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Dear confocal list--

Is anyone aware of a means by which ordinary glass fibers can be
fluorescently labeled?  (--I'd prefer not going the route of a negative
stain if possible.)  They'll be present in a cellulose matrix, which
ideally would NOT be labeled by the stain.

Thanks--

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
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[John Oreopoulos]

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Hi Martin,

I don't know of a way to stain glass, but perhaps you could use something like these hollow glass capillary fibers made by VitroCom (no commercial interest):

http://www.vitrocom.com/categories/view/17/Borosilicate

Could you fill these with a fluorescent dye and then embed them in your sample?

John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca


On 2011-08-29, at 12:57 PM, Martin Wessendorf wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear confocal list--
>
> Is anyone aware of a means by which ordinary glass fibers can be fluorescently labeled?  (--I'd prefer not going the route of a negative stain if possible.)  They'll be present in a cellulose matrix, which ideally would NOT be labeled by the stain.
>
> Thanks--
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]

[mmodel]

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Maybe fluorescent poly-lysine? Looks like such things exist, though expensive

Mike

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
Sent: Monday, August 29, 2011 12:58 PM
To: [hidden email]
Subject: fluorescent stain for glass

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Dear confocal list--

Is anyone aware of a means by which ordinary glass fibers can be
fluorescently labeled?  (--I'd prefer not going the route of a negative
stain if possible.)  They'll be present in a cellulose matrix, which
ideally would NOT be labeled by the stain.

Thanks--

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Kurt Thorn]

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Another possibility would be to fluoresently label a silane and then use
the labeled silane to label the glass.  No idea how specific it would be
for glass over cellulose.

Kurt

On 8/29/2011 10:17 AM, MODEL, MICHAEL wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Maybe fluorescent poly-lysine? Looks like such things exist, though expensive
>
> Mike
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
> Sent: Monday, August 29, 2011 12:58 PM
> To: [hidden email]
> Subject: fluorescent stain for glass
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear confocal list--
>
> Is anyone aware of a means by which ordinary glass fibers can be
> fluorescently labeled?  (--I'd prefer not going the route of a negative
> stain if possible.)  They'll be present in a cellulose matrix, which
> ideally would NOT be labeled by the stain.
>
> Thanks--
>
> Martin Wessendorf

[mcammer]

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There must be organic dyes that will stick to the glass (if this is permissible).  For instance, some Sharpie marker inks are fluorescent.  Perhaps this could be a really cheap and easy way to solve the problem.
-Michael C
_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Martin Wessendorf [[hidden email]]
Sent: Monday, August 29, 2011 12:57 PM
To: [hidden email]
Subject: fluorescent stain for glass

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Dear confocal list--

Is anyone aware of a means by which ordinary glass fibers can be
fluorescently labeled?  (--I'd prefer not going the route of a negative
stain if possible.)  They'll be present in a cellulose matrix, which
ideally would NOT be labeled by the stain.

Thanks--

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

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[Neeraj Gohad-2]

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You could first silanize the glass tubes, you get silanes with different end groups, you could choose an appropriate one and then choose a fluorophore with a matching end group and use one of the well know conjugation chemistries carbonylimidazole (CDI) or disuccinimidylcarbonate (DSC) to conjugate it to the silane on the glass.

Best,

Neeraj.


Neeraj V. Gohad, Ph.D.
Research Assistant Professor
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: [phone]
Fax: [phone]




From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
Sent: Monday, August 29, 2011 12:58 PM
To: [hidden email]
Subject: fluorescent stain for glass

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Dear confocal list--

Is anyone aware of a means by which ordinary glass fibers can be
fluorescently labeled?  (--I'd prefer not going the route of a negative
stain if possible.)  They'll be present in a cellulose matrix, which
ideally would NOT be labeled by the stain.

Thanks--

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Oshel, Philip Eugene]

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Do you need to use fluorescence, or just image the glass within the
cellulose? If the latter, Rheinberg illumination might work -
basically darkfield with colored light (colored central stop instead
of opaque, different color for the annular aperture).
This would depend on a RI difference between the glass fibers and the
cellulose, but would not alter either the glass or cellulose, which
might be useful.

Phil

>
>Dear confocal list--
>
>Is anyone aware of a means by which ordinary glass fibers can be
>fluorescently labeled?  (--I'd prefer not going the route of a
>negative stain if possible.)  They'll be present in a cellulose
>matrix, which ideally would NOT be labeled by the stain.
>
>Thanks--
>
>Martin Wessendorf
>--
>Martin Wessendorf, Ph.D.                   office: [phone]
>Assoc Prof, Dept Neuroscience                 lab: [phone]
>University of Minnesota             Preferred FAX: [phone]
>6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
>Minneapolis, MN  55455                    e-mail: [hidden email]

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
[phone]

[Zac Arrac Atelaz]

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Martin:
 
If you can mix some "vaseline glass" and with the glas fibers, that can help you, you will have green light back.
 
Regards
 

 

> Date: Mon, 29 Aug 2011 11:57:36 -0500
> From: [hidden email]
> Subject: fluorescent stain for glass
> To: [hidden email]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear confocal list--
>
> Is anyone aware of a means by which ordinary glass fibers can be
> fluorescently labeled? (--I'd prefer not going the route of a negative
> stain if possible.) They'll be present in a cellulose matrix, which
> ideally would NOT be labeled by the stain.
>
> Thanks--
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D. office: [phone]
> Assoc Prof, Dept Neuroscience lab: [phone]
> University of Minnesota Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE Dept Fax: [phone]
> Minneapolis, MN 55455 e-mail: [hidden email]

     

[Lloyd Donaldson]

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Martin

We have examined similar composites using mixed techniques. Reflectance will show all the fibres, then you just need to differentiate the cellulose from the glass. Cellulose can be stained with calcofluor or congo red, or you can image the cellulose by widefield polarisation and use extended focus software or an average intensity projection to get some z-dimension.
An alternative (and probably much better) approach would be to image the sample with SEM using backscattered electrons with atomic number contrast.

Regards

Dr Lloyd Donaldson

Senior Scientist, Project Leader - Microscopy/Wood Identification
Scion - Forests . Products . Innovation
Private Bag 3020, Rotorua
49 Sala Street, Rotorua 3010
New Zealand

Ph: 64 7 343 5581
www.scionresearch.com



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
Sent: Tuesday, 30 August 2011 4:58 a.m.
To: [hidden email]
Subject: fluorescent stain for glass

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Dear confocal list--

Is anyone aware of a means by which ordinary glass fibers can be
fluorescently labeled?  (--I'd prefer not going the route of a negative
stain if possible.)  They'll be present in a cellulose matrix, which
ideally would NOT be labeled by the stain.

Thanks--

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]



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[Barbara Foster]

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Have you considered using polarized light?  The cellulose should
respond beautifully to Pol, while the glass fiber should either
exhibit weak or no birefringence.  On recommendation: use circularly
polarized light to remove the orientation effect of the fibers.  Easy
and inexpensive.

Good hunting!
Barbara Foster, President and Sr. Consultant

Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: [phone]  Skype: fostermme
W: www.MicroscopyEducation.com



At 05:33 PM 8/29/2011, you wrote:

>*****
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>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Another possibility would be to fluoresently label a silane and then
>use the labeled silane to label the glass.  No idea how specific it
>would be for glass over cellulose.
>
>Kurt
>
>On 8/29/2011 10:17 AM, MODEL, MICHAEL wrote:
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>*****
>>
>>Maybe fluorescent poly-lysine? Looks like such things exist, though expensive
>>
>>Mike
>>
>>-----Original Message-----
>>From: Confocal Microscopy List
>>[mailto:[hidden email]] On Behalf Of Martin Wessendorf
>>Sent: Monday, August 29, 2011 12:58 PM
>>To: [hidden email]
>>Subject: fluorescent stain for glass
>>
>>*****
>>To join, leave or search the confocal microscopy listserv, go to:
>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>*****
>>
>>Dear confocal list--
>>
>>Is anyone aware of a means by which ordinary glass fibers can be
>>fluorescently labeled?  (--I'd prefer not going the route of a negative
>>stain if possible.)  They'll be present in a cellulose matrix, which
>>ideally would NOT be labeled by the stain.
>>
>>Thanks--
>>
>>Martin Wessendorf

[Mario]

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Martin,

To provide a practical solution for labeling glass, 1) it would be
helpful to know more details concerning what environment the glass
would be immersed in including pH, electrolyte concentrations,
electrostatics of the glass and cellulose matrix; 2) what sort of
glass objects are to be imaged such as spheres, fibers, chips, etc.;
and 3) what kind of limitations are there regarding initial prepping
of the glass surfaces.

If it is possible to pretreat the glass in the specimen with amino
propyl silane without adversely affecting the interaction of the
glass with the matrix, then you have lots of options. For example,
the aminated glass can then be biotinylated making it a good target
for an avidin/streptavidin fluorescent label (color of your choice).
A fluorophore tagged anti-biotin antibody should also work. Further,
if the glass can also be pre-treated with a sulfhydryl terminated
silane, the latter can then be conjugated with fluorescein-maleimide,
about the brightest fluorescein label available.

If the glass can be coupled with dsDNA, then you could label with
DAPI or an appropriate Hoechst stain.

In any case, to select the best labeling strategy it would be helpful
to know just how sticky the glass fibers might be. Incubating a
sample of glass fibers for 15 minutes in a labeling solution followed
by three 20 minute rinses should give an idea just how stable binding
of the dye will be.

Martin there are many other tricks but a little more info should help
lead to more specific approaches.

Mario

>*****
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>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Dear confocal list--
>
>Is anyone aware of a means by which ordinary glass fibers can be
>fluorescently labeled?  (--I'd prefer not going the route of a
>negative stain if possible.)  They'll be present in a cellulose
>matrix, which ideally would NOT be labeled by the stain.
>
>Thanks--
>
>Martin Wessendorf
>--
>Martin Wessendorf, Ph.D.                   office: [phone]
>Assoc Prof, Dept Neuroscience                 lab: [phone]
>University of Minnesota             Preferred FAX: [phone]
>6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
>Minneapolis, MN  55455                    e-mail: [hidden email]

--
_________________________________________________________________
Mario M. Moronne, Ph.D.
Scientific Consulting
[hidden email]
[hidden email]
ph [phone]

[Jan Klepetar]

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Hi Martin,
Any surface staining can change the physico-chemical properties of glass
surface. If you needn´t care about this problem - alcoholic solution of
Rhodamin6G (or some other FP...) + shellac can help you. Shellac prevents
the dissolution of fluorophore in water. I apply red permanent marker on
glass surfaces for some purposes.
If you have to care about the cell-surface interaction, the suitably
coloured glass is the solution... some of pigments used in glass industry
can be fluorescent.
Jan Klepetar


RNDr. Jan Klepetar
Dept.Biomathematics,Inst.Physiology
Academy of Sciences CR
Videnska 1083, 14220 Prague 4
Czech Republic.




-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Martin Wessendorf
Sent: Monday, August 29, 2011 6:58 PM
To: [hidden email]
Subject: fluorescent stain for glass

*****
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Dear confocal list--

Is anyone aware of a means by which ordinary glass fibers can be
fluorescently labeled?  (--I'd prefer not going the route of a negative
stain if possible.)  They'll be present in a cellulose matrix, which
ideally would NOT be labeled by the stain.

Thanks--

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Martin Wessendorf-2]

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Thanks to all who responded.  At this point, I need to huddle with our
users to determine the best tactic but I appreciate the variety of ideas
that we now have to choose from!

Martin

On 8/30/2011 2:22 AM, Jan Klepetar wrote:

> Hi Martin,
> Any surface staining can change the physico-chemical properties of glass
> surface. If you needn´t care about this problem - alcoholic solution of
> Rhodamin6G (or some other FP...) + shellac can help you. Shellac prevents
> the dissolution of fluorophore in water. I apply red permanent marker on
> glass surfaces for some purposes.
> If you have to care about the cell-surface interaction, the suitably
> coloured glass is the solution... some of pigments used in glass industry
> can be fluorescent.
> Jan Klepetar
>
>
> RNDr. Jan Klepetar
> Dept.Biomathematics,Inst.Physiology
> Academy of Sciences CR
> Videnska 1083, 14220 Prague 4
> Czech Republic.
>
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On
> Behalf Of Martin Wessendorf
> Sent: Monday, August 29, 2011 6:58 PM
> To: [hidden email]
> Subject: fluorescent stain for glass
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear confocal list--
>
> Is anyone aware of a means by which ordinary glass fibers can be
> fluorescently labeled?  (--I'd prefer not going the route of a negative
> stain if possible.)  They'll be present in a cellulose matrix, which
> ideally would NOT be labeled by the stain.
>
> Thanks--
>
> Martin Wessendorf

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Jurkevic, Aleksandr]

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Hello All,

This is not quite a microscopy question. At our core we have a sample preparation lab where clients can section their tissue samples. An investigator wants to cut cryosections of human tissue (skin). We are primarily a research facility and not a clinical lab but I do not see a problem letting him to cut the sections providing that samples are properly formalin-fixed and he has approved protocols for his procedures. Am I oversimplifying the issue and are there any biosafety aspects that have to be considered? I would appreciate if you could share your policies on dealing with human tissue samples.

Thank you.


Aleksandr Jurkevic, PhD
Associate Director
Molecular Cytology Core
University of Missouri-Columbia
120 Life Sciences Center
1201 E. Rollins St.
Columbia, MO 65211-7310

Phone:    [phone]
Fax:           [phone]
website  http://www.biotech.missouri.edu/mcc/

 

[Greg Martin-8]

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Hey Aleksandr et. al. --

    I would just make sure it comes into the lab fixed, and ask for a document describing their fixation protocol which you then file away.  If you're satisfied (well, if your Biosafety people are satisfied) that the specimen is rendered non-potentially-infectious, let 'em cut to their hearts content.  But make sure they clean up the cryostat really well  --  remember your other users will most likely be oblivious to the fact that human tissue was cut on that cryostat.  It might be proper and fitting to post a notice...

Be peace!  Greg.

Greg Martin

Keck Microscopy Facility
University of Washington School of Medicine
www.depts.washington.edu/keck

206-685-8784 (office)
425-344-2632 (cell)
----- Original Message -----
From: "Jurkevic, Aleksandr" <[hidden email]>
To: <[hidden email]>
Sent: Tuesday, August 30, 2011 3:54 PM
Subject: human tissue sections


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Hello All,

This is not quite a microscopy question. At our core we have a sample preparation lab where clients can section their tissue samples. An investigator wants to cut cryosections of human tissue (skin). We are primarily a research facility and not a clinical lab but I do not see a problem letting him to cut the sections providing that samples are properly formalin-fixed and he has approved protocols for his procedures. Am I oversimplifying the issue and are there any biosafety aspects that have to be considered? I would appreciate if you could share your policies on dealing with human tissue samples.

Thank you.


Aleksandr Jurkevic, PhD
Associate Director
Molecular Cytology Core
University of Missouri-Columbia
120 Life Sciences Center
1201 E. Rollins St.
Columbia, MO 65211-7310

Phone: [phone]
Fax: [phone]
website http://www.biotech.missouri.edu/mcc/

 

[Craig Brideau]

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I think you should check this with the safety office of your institute.
 Wouldn't they be familiar with the applicable regulations?  The rules on
this sort of thing vary from area to area so advice from a broad forum like
this could get you into trouble.

Craig


On Tue, Aug 30, 2011 at 4:54 PM, Jurkevic, Aleksandr <[hidden email]
> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
>
> This is not quite a microscopy question. At our core we have a sample
> preparation lab where clients can section their tissue samples. An
> investigator wants to cut cryosections of human tissue (skin). We are
> primarily a research facility and not a clinical lab but I do not see a
> problem letting him to cut the sections providing that samples are properly
> formalin-fixed and he has approved protocols for his procedures. Am I
> oversimplifying the issue and are there any biosafety aspects that have to
> be considered? I would appreciate if you could share your policies on
> dealing with human tissue samples.
>
> Thank you.
>
>
> Aleksandr Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri-Columbia
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone:    [phone]
> Fax:           [phone]
> website  http://www.biotech.missouri.edu/mcc/
>
>
>

[Michelle Aloni]

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*****

Hi Aleksandr,

I am histotechnologist and have to add my two cents here.

If your client wants to do cryosectioning, he must have a special reason to use the tissue fresh. This would be why he is doing cryosectioning instead of paraffin sectioning.
This also means that the tissue won't be fixed at all, but fresh frozen instead.

Skin is not the easiest tissue to section frozen in a first place. If you make him/her use fixed tissue in the cryostat, first - it will be hard to keep the sections on the slide during staining (especially it it is immuno stain) and second, if the tissue is already fixed, the morphology would look much better if the tissue is embedded and sectioned in paraffin.

As far as safety, call for a protocol the closest Hospital. Any Histology lab in a Hospital must have a written protocol for work with frozen human tissue. This is what they do on a daily basis. They would gladly fax or e-mail it to you.

If you have any other histology related questions, let me know. I will be glad to help.

Michelle Aloni MS, HTL (ASCP)
GI and Liver - Cell and Tissue Imaging Core
USC Keck School of Medicine
2011 Zonal Ave. room 610
Los Angeles, CA 90033

Phone: [phone]

[tineke vendrig]

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Hello,

I think when you go to a hospital/medical pathological laboratory, they can
tell you, that, when you clean everything before and after sectioning with
alcohol, nothing will happen! And also leave nothin inside, so all sections
have to be removed, also from the sides (no 'flying'  sections). Let him
eventually use his own knives...
But you have to be sure, that this will happen!

Good luck!

Tineke Vendrig
Research technician

Delft University of Technology
Faculty of Applied Science
Kavli Institute of NanoScience
Department of Bionanoscience (BN)
Lorentzweg 1
2628 CJ Delft
The Netherlands

phone: [phone]
fax: [phone]

e-mail:[hidden email]
webpage: www.bn.tudelft.nl

2011/8/31 Jurkevic, Aleksandr <[hidden email]>

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
>
> This is not quite a microscopy question. At our core we have a sample
> preparation lab where clients can section their tissue samples. An
> investigator wants to cut cryosections of human tissue (skin). We are
> primarily a research facility and not a clinical lab but I do not see a
> problem letting him to cut the sections providing that samples are properly
> formalin-fixed and he has approved protocols for his procedures. Am I
> oversimplifying the issue and are there any biosafety aspects that have to
> be considered? I would appreciate if you could share your policies on
> dealing with human tissue samples.
>
> Thank you.
>
>
> Aleksandr Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri-Columbia
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone:    [phone]
> Fax:           [phone]
> website  http://www.biotech.missouri.edu/mcc/
>
>
>

[Zac Arrac Atelaz]

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Aleksandr:
 
We have almost every week live cancer cells (HeLa) observed at our core facility, I see no big care taken when handling those, I am almost sure this is lackadaisical care is going to charge a fee on someone eventually but as this is not Immediate and mortal at the same time, contermeasures are going to be neglected some time, but when your sample is fixed, there is only extremophiles living there until formalin evaporates or get washed.
 
Regards.
 
Gabriel OH

 

> Date: Tue, 30 Aug 2011 17:54:37 -0500
> From: [hidden email]
> Subject: human tissue sections
> To: [hidden email]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
>
> This is not quite a microscopy question. At our core we have a sample preparation lab where clients can section their tissue samples. An investigator wants to cut cryosections of human tissue (skin). We are primarily a research facility and not a clinical lab but I do not see a problem letting him to cut the sections providing that samples are properly formalin-fixed and he has approved protocols for his procedures. Am I oversimplifying the issue and are there any biosafety aspects that have to be considered? I would appreciate if you could share your policies on dealing with human tissue samples.
>
> Thank you.
>
>
> Aleksandr Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri-Columbia
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone:    [phone]
> Fax:           [phone]
> website  http://www.biotech.missouri.edu/mcc/
>
>

     

[Danielle Crippen]

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The need to cryosection most surely means the tissue won't be fixed...maybe they will need ORO or Betagal staining or something along those lines that requires fresh frozen tissue.

As long as the tissue isn't positive for HIV (or some other virus)which might require additional guidelines, I would approach this situation in the following manner:

1. call the local hospital for their procedure--great suggestion of all who made it!
2. check with your Biosafety committee for their recommendations; check whether there's anything in your current BUA that can guide you -- maybe you need to amend your BUA??
3. once cleared, give the user his own blades and make sure they only use these
4. make sure to clean everything off after each use with unfixed human tissue with 70% EtOH, then 100% EtOh (the latter for complete drying in the chamber)
5. if you have a UV lamp in your cryostat, use it after each use for 30 minutes or so.

Best of luck!

d


_______________________________
Danielle Crippen
Morphology and Imaging Core Manager
Buck Institute for Research on Aging
8001 Redwood Blvd
Novato, CA 94945
415-209-2046
TheBuck.org
 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Zac Arrac Atelaz
Sent: Wednesday, August 31, 2011 8:22 AM
To: [hidden email]
Subject: Re: human tissue sections

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To join, leave or search the confocal microscopy listserv, go to:
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Aleksandr:
 
We have almost every week live cancer cells (HeLa) observed at our core facility, I see no big care taken when handling those, I am almost sure this is lackadaisical care is going to charge a fee on someone eventually but as this is not Immediate and mortal at the same time, contermeasures are going to be neglected some time, but when your sample is fixed, there is only extremophiles living there until formalin evaporates or get washed.
 
Regards.
 
Gabriel OH

 

> Date: Tue, 30 Aug 2011 17:54:37 -0500
> From: [hidden email]
> Subject: human tissue sections
> To: [hidden email]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
>
> This is not quite a microscopy question. At our core we have a sample preparation lab where clients can section their tissue samples. An investigator wants to cut cryosections of human tissue (skin). We are primarily a research facility and not a clinical lab but I do not see a problem letting him to cut the sections providing that samples are properly formalin-fixed and he has approved protocols for his procedures. Am I oversimplifying the issue and are there any biosafety aspects that have to be considered? I would appreciate if you could share your policies on dealing with human tissue samples.
>
> Thank you.
>
>
> Aleksandr Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri-Columbia
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone:    [phone]
> Fax:           [phone]
> website  http://www.biotech.missouri.edu/mcc/
>
>