fluorescent staining of starch
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Valerie Lawrence |
fluorescent staining of starch
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi all, We are looking for a method of imaging starch in paper using confocal microscopy. None of the fluorescent dyes we’ve tried (basic fuchsin, crystal violet, SF light) are useful since they also stain the cellulose in the wood fibres. Starch has been hydrolyzed. Any suggestions would be greatly appreciated. Thank you. Valerie Lawrence |
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John Oreopoulos |
Re: fluorescent staining of starch
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Valerie, I seem to remember this question coming up a few times over the years on the listserver. You could try a keyword search for "starch" on the archive: http://confocal-microscopy-list.588098.n2.nabble.com/template/NamlServlet.jtp?macro=adv_search_page&node=588098 John Oreopoulos Research Assistant Spectral Applied Research Richmond Hill, Ontario Canada L4B 3L8 On 2011-02-18, at 2:48 PM, Valerie Lawrence wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi all, > We are looking for a method of imaging starch in paper using confocal > microscopy. None of the fluorescent dyes we’ve tried (basic fuchsin, crystal > violet, SF light) are useful since they also stain the cellulose in the wood > fibres. Starch has been hydrolyzed. Any suggestions would be greatly > appreciated. > Thank you. > Valerie Lawrence |
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In reply to this post by Valerie Lawrence
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Valerie, By far the simplest approach is to use second harmonic imaging. Starch gives a very strong SH signal. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm <http://www.guycox.com/optical.htm> ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net <http://www.guycox.net> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Valerie Lawrence Sent: Saturday, 19 February 2011 6:49 AM To: [hidden email] Subject: fluorescent staining of starch ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi all, We are looking for a method of imaging starch in paper using confocal microscopy. None of the fluorescent dyes we've tried (basic fuchsin, crystal violet, SF light) are useful since they also stain the cellulose in the wood fibres. Starch has been hydrolyzed. Any suggestions would be greatly appreciated. Thank you. Valerie Lawrence ________________________________ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1204 / Virus Database: 1435/3450 - Release Date: 02/17/11 |
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Elizabeth Nickless |
Re: fluorescent staining of starch
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Would second harmonic imaging show up processed starch though? Polarised light shows up raw starch grains but will it work if the grains have taken up water and changed structure? They lose the maltese cross which usually shows up in polarised light. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Guy Cox Sent: Sunday, 20 February 2011 12:45 a.m. To: [hidden email] Subject: Re: fluorescent staining of starch ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Valerie, By far the simplest approach is to use second harmonic imaging. Starch gives a very strong SH signal. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm <http://www.guycox.com/optical.htm> ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net <http://www.guycox.net> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Valerie Lawrence Sent: Saturday, 19 February 2011 6:49 AM To: [hidden email] Subject: fluorescent staining of starch ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi all, We are looking for a method of imaging starch in paper using confocal microscopy. None of the fluorescent dyes we've tried (basic fuchsin, crystal violet, SF light) are useful since they also stain the cellulose in the wood fibres. Starch has been hydrolyzed. Any suggestions would be greatly appreciated. Thank you. Valerie Lawrence ________________________________ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1204 / Virus Database: 1435/3450 - Release Date: 02/17/11 DISCLAIMER This email contains information that is confidential and which may be legally privileged. If you have received this email in error, please notify the sender immediately and delete the email. This email is intended solely for the use of the intended recipient and you may not use or disclose this email in any way. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** The Maltese Cross is a function of the radial orientation of the starch molecules and has nothing to with either their inherent birefringence or their ability to generate SH. In general processed starch tends to be less crystalline and therefore has a weaker (but still clear) SHG signal. Subsequent changes (such as baked products becoming stale) lead to an increase in crystallinity and therefore a brighter signal - which can be useful to food scientists. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm <http://www.guycox.com/optical.htm> ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net <http://www.guycox.net> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Elizabeth Nickless Sent: Monday, 21 February 2011 11:38 AM To: [hidden email] Subject: Re: fluorescent staining of starch ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Would second harmonic imaging show up processed starch though? Polarised light shows up raw starch grains but will it work if the grains have taken up water and changed structure? They lose the maltese cross which usually shows up in polarised light. -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Guy Cox Sent: Sunday, 20 February 2011 12:45 a.m. To: [hidden email] Subject: Re: fluorescent staining of starch ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Valerie, By far the simplest approach is to use second harmonic imaging. Starch gives a very strong SH signal. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm <http://www.guycox.com/optical.htm> ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Australian Centre for Microscopy & Microanalysis, Madsen Building F09, University of Sydney, NSW 2006 Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net <http://www.guycox.net> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Valerie Lawrence Sent: Saturday, 19 February 2011 6:49 AM To: [hidden email] Subject: fluorescent staining of starch ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi all, We are looking for a method of imaging starch in paper using confocal microscopy. None of the fluorescent dyes we've tried (basic fuchsin, crystal violet, SF light) are useful since they also stain the cellulose in the wood fibres. Starch has been hydrolyzed. Any suggestions would be greatly appreciated. Thank you. Valerie Lawrence ________________________________ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1204 / Virus Database: 1435/3450 - Release Date: 02/17/11 DISCLAIMER This email contains information that is confidential and which may be legally privileged. If you have received this email in error, please notify the sender immediately and delete the email. This email is intended solely for the use of the intended recipient and you may not use or disclose this email in any way. ________________________________ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1204 / Virus Database: 1435/3456 - Release Date: 02/20/11 |
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