good anti-GFP antibody for immunocytochemistry

[lechristophe]

Dear all,

Sorry for this non-confocal question, but I guessed some of you might be able to recommend an anti-GFP antibody for immunocytochemistry. I want to label cultured cells that have been transfected with a GFP fusion protein (to enhance the GFP signal). I routinely use the Rockland anti-RFP for the same protein fused to mCherry, but now I would like to use the GFP fusion and the signal is a bit weak.

Thank you !

Christophe


--
Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
[hidden email]

[Michael Schell]

I suggest the mouse anti-GFP monoclonal clone 3E6.  It is sold by a
number of different vendors, including Molecular Probes.  It works great
for enhancing the GFP signal in PFA-fixed cells and tissues.

Michael

Michael J. Schell, Ph.D., CIV, USUHS
Assist. Professor
Dept. of Pharmacology
Uniformed Services University
4301 Jones Bridge Rd.
Bethesda, MD  20814-3220
tel:  [phone]
[hidden email]
>>> Christophe Leterrier <[hidden email]> 10/05/09 10:20
AM >>>
Dear all,

Sorry for this non-confocal question, but I guessed some of you might be
able to recommend an anti-GFP antibody for immunocytochemistry. I want
to
label cultured cells that have been transfected with a GFP fusion
protein
(to enhance the GFP signal). I routinely use the Rockland anti-RFP for
the
same protein fused to mCherry, but now I would like to use the GFP
fusion
and the signal is a bit weak.

Thank you !

Christophe


--
Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
[hidden email]

[Ignatius, Mike-2]

Dear Christophe,

 

Molecular Probes/Invitrogen sells quite a few versions of anti-GFP antibodies.

 

If you type in anti-GFP in our browser you can see the complete list.  mAbs, polys, direct conjugates in many Alexa Fluor colors, biotinXX, HRP, even chicken IgY (no Fc tail so low backgrounds).  Some optimized for westerns, most for all applications, especially ICC.   All raised to A. victoria FPs.

 

Regards,

 

Mike Ignatius

 

Molecular Probes/Invitrogen/Life Technologies

 

 

---

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Christophe Leterrier
Sent: Monday, October 05, 2009 7:10 AM
To: [hidden email]
Subject: good anti-GFP antibody for immunocytochemistry

Dear all,

Sorry for this non-confocal question, but I guessed some of you might be able to recommend an anti-GFP antibody for immunocytochemistry. I want to label cultured cells that have been transfected with a GFP fusion protein (to enhance the GFP signal). I routinely use the Rockland anti-RFP for the same protein fused to mCherry, but now I would like to use the GFP fusion and the signal is a bit weak.

Thank you !

Christophe


--
Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
[hidden email]

[Won Yung Choi, Ph.D.]

Dear Christophe,

I have very good success with the anti-GFP from Chemicon as well (rabbit
poly AB3080).  It also binds to GFP variants such as CFP (for instance, when
tested in brainbow mice).  The signal is exceptionally strong - you might
even have to dilute it more than what the manufacturer suggests.  Good luck!

------------------------------------------------------------
Won Yung Choi, Ph.D.  
Adjunct Research Scientist      
Department of Psychiatry                          
Columbia University                                      
1051 Riverside Drive, NYSPI Unit 62        
New York, NY 10032 [phone]
------------------------------------------------------------

On Mon, 5 Oct 2009 12:09:56 -0400, Ignatius, Mike
<[hidden email]> wrote:

>Dear Christophe,
>
>Molecular Probes/Invitrogen sells quite a few versions of anti-GFP antibodies.
>
>If you type in anti-GFP in our browser you can see the complete list.
mAbs, polys, direct conjugates in many Alexa Fluor colors, biotinXX, HRP,
even chicken IgY (no Fc tail so low backgrounds).  Some optimized for
westerns, most for all applications, especially ICC.   All raised to A.
victoria FPs.

>
>Regards,
>
>Mike Ignatius
>
>Molecular Probes/Invitrogen/Life Technologies
>
>
>
>________________________________
>From: Confocal Microscopy List [mailto:[hidden email]] On

Behalf Of Christophe Leterrier
>Sent: Monday, October 05, 2009 7:10 AM
>To: [hidden email]
>Subject: good anti-GFP antibody for immunocytochemistry
>
>Dear all,
>
>Sorry for this non-confocal question, but I guessed some of you might be
able to recommend an anti-GFP antibody for immunocytochemistry. I want to
label cultured cells that have been transfected with a GFP fusion protein
(to enhance the GFP signal). I routinely use the Rockland anti-RFP for the
same protein fused to mCherry, but now I would like to use the GFP fusion
and the signal is a bit weak.

>
>Thank you !
>
>Christophe
>
>
>--
>Christophe Leterrier
>Postdoc
>INSERM UMR641 // Ionic channels Lab
>IFR Jean Roche, Mediterranée University
>Marseille, France
>[hidden email]<mailto:[hidden email]>
>
>