Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalThe difference will come with the SA correction. TIRF
lenses (at least the Leica one) actually have values for
different temperatures on the correction ring. This is
working with oil, which changes RI rather a lot with
temperature. In water the difference will be smaller, but
it's still worth setting the correction collar visually
rather than by trusting the scale. Once you have set it
correctly there should be no real difference in PSF.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Carl Boswell
Sent: Friday, 8 February 2008 5:58 AM
To:
[hidden email]
Subject: heat and PSF
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHi all,
Does anyone have experience with characterizing PSF's (or other aspects of the optics) at different temperatures? For instance, how much effect is there on an objective when the temp is raised from room temp (25C) to physiologically relevant 37C?
Thanks,
Carl
Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709
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