Cammer, Michael |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We are submitting a request for a new lab which requires reporting heat load in BTU/hr or watts to attempt proper air handling. We have a few forced air incubation chambers such as the Weatherstation, Zeiss, and other large boxes that surround the microscope stage. Does anyone know what are what are acceptable, reasonable, or accurate numbers for 37 degrees C? The boxes are minimally insulated. Also, what are people reporting for metal halide lamps (such as X-Cite or Leica EL6000)? (For HBO 100 back in the 1990s we said 1250 BTU/hr, but I don't know where this number came from.) Any help appreciated. Thank you! Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016 Office: 646-501-0567 Cell: 914-309-3270 [hidden email]<mailto:[hidden email]> http://nyulmc.org/micros http://microscopynotes.com/ Acknowledgement in your publications and presentations of work performed in the Microscopy Core plays a vital role in securing support and the funding necessary to maintain and operate this valuable research resource. For publications that were made possible by work performed in the core, please use the acknowledgement statement "We thank the NYU Langone Microscopy Core for experimental and technical support" and include required grant numbers as listed here http://microscopynotes.com/ilabnyu/acknowledgements2017.pdf Please also consider staff for co-authorship if they played a key role in the study. |
Zdenek Svindrych-2 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** It's simple, for every Watt of electricity consumed, there is one Watt (or 3.4 BTU/hr) load to the HVAC. So a 100 W light source (with 80% power source efficiency) is around 400 BTU/hr. That would be the number to allocate for HBO 100 or XCite 120. Btw, one person (sitting and not thinking too hard :-) is also 400 BTU/hr. Best, zdenek On Tue, Dec 17, 2019 at 10:52 AM Cammer, Michael < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > > We are submitting a request for a new lab which requires reporting heat > load in BTU/hr or watts to attempt proper air handling. > > We have a few forced air incubation chambers such as the Weatherstation, > Zeiss, and other large boxes that surround the microscope stage. Does > anyone know what are what are acceptable, reasonable, or accurate numbers > for 37 degrees C? The boxes are minimally insulated. > > Also, what are people reporting for metal halide lamps (such as X-Cite or > Leica EL6000)? (For HBO 100 back in the 1990s we said 1250 BTU/hr, but I > don't know where this number came from.) > > Any help appreciated. > > Thank you! > > > Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory > NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY > 10016 > Office: 646-501-0567 Cell: 914-309-3270 [hidden email] > <mailto:[hidden email]> > http://nyulmc.org/micros http://microscopynotes.com/ > Acknowledgement in your publications and presentations of work performed > in the Microscopy Core plays a vital role in securing support and the > funding necessary to maintain and operate this valuable research resource. > For publications that were made possible by work performed in the core, > please use the acknowledgement statement "We thank the NYU Langone > Microscopy Core for experimental and technical support" and include > required grant numbers as listed here > http://microscopynotes.com/ilabnyu/acknowledgements2017.pdf > Please also consider staff for co-authorship if they played a key role in > the study. > -- -- Zdenek Svindrych, Ph.D. Research Associate - Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth |
In reply to this post by Cammer, Michael
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Michael I cannot tell you how much heat load a boxed microscope creates but I can tell you what the energy load is of Bioptechs Microenvironmental Control Systems. You will find it is considerably less, easier to work with and more functional. We study heat propagation very closely and develop specimen control systems for long term time lapse in a manner that specifically controls the specimen without requiring a box with warm, humidified air being pumped into an expensive microscope. (In a former life as a microscope service technician, I hated to have to work on restoring an environmentally boxed scope)! As a point of reference: In the case of a closed system, laminar flow cell for upright or inverted scopes, held steady and uniform at 37°C, the power consumption is about 2 watts, if thermally disturbed equilibrium occurs in about 15 seconds. A properly implemented objective warmer uses about 1.5 watts and a Z axis stable contents of a culture dish can be maintained with 0.3 - 0.5 watts. These techniques are so efficient that they don't require specialized infrastructure. Even if you use a box around the scope or on the stage we can cut down the equilibration time down to a couple of minutes. The main reason for boxing the entire scope is stability however, I have been proving for 27 years that with a dedicated environmental system that is properly engineered, you don't have to be physically encumbered, heating, gassing, and humidifying a scope in a "box”. If you contact us and tell us what you intend to do and what equipment you have to work with, we can send you a proposal for a solution. Note, we are not looking for intellectual property, we only need enough info to properly address your needs. Dan Dan Focht Bioptechs Inc. 3560 Beck Road Butler, PA 16002-9259 Office: 724-282-7145 Toll Free: 877-LIVE-CELL (548-3235) [hidden email] www.bioptechs.com On Dec 17, 2019, at 10:29 AM, Cammer, Michael <[hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We are submitting a request for a new lab which requires reporting heat load in BTU/hr or watts to attempt proper air handling. We have a few forced air incubation chambers such as the Weatherstation, Zeiss, and other large boxes that surround the microscope stage. Does anyone know what are what are acceptable, reasonable, or accurate numbers for 37 degrees C? The boxes are minimally insulated. Also, what are people reporting for metal halide lamps (such as X-Cite or Leica EL6000)? (For HBO 100 back in the 1990s we said 1250 BTU/hr, but I don't know where this number came from.) Any help appreciated. Thank you! Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY 10016 Office: 646-501-0567 Cell: 914-309-3270 [hidden email]<mailto:[hidden email]> http://nyulmc.org/micros http://microscopynotes.com/ Acknowledgement in your publications and presentations of work performed in the Microscopy Core plays a vital role in securing support and the funding necessary to maintain and operate this valuable research resource. For publications that were made possible by work performed in the core, please use the acknowledgement statement "We thank the NYU Langone Microscopy Core for experimental and technical support" and include required grant numbers as listed here http://microscopynotes.com/ilabnyu/acknowledgements2017.pdf Please also consider staff for co-authorship if they played a key role in the study. |
Cammer, Michael |
In reply to this post by Zdenek Svindrych-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thank you for the public and private replies. A lot of people replied regarding the bulbs, and the 80% efficiency number below was particularly helpful, but we may go the ammeter approach. If we measure ourselves, I will post the results. Otherwise, thank you again. Cheers- Michael C. -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Zdenek Svindrych Sent: Tuesday, December 17, 2019 12:01 PM To: [hidden email] Subject: Re: heat load question for new lab [EXTERNAL] ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=7WFp_sPsfcSMHAePbKGxGBycZV5ThfIe8hNPvG7WqwY&s=SSPamQlUriyGMaTWlgoS4pwctCqc39W4dGGLloesVyw&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=7WFp_sPsfcSMHAePbKGxGBycZV5ThfIe8hNPvG7WqwY&s=H3cSBvdDft_Ax5MWoB5oOddWtGcc5YroRzTh3U8rToY&e= and include the link in your posting. ***** It's simple, for every Watt of electricity consumed, there is one Watt (or 3.4 BTU/hr) load to the HVAC. So a 100 W light source (with 80% power source efficiency) is around 400 BTU/hr. That would be the number to allocate for HBO 100 or XCite 120. Btw, one person (sitting and not thinking too hard :-) is also 400 BTU/hr. Best, zdenek On Tue, Dec 17, 2019 at 10:52 AM Cammer, Michael < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi- > 2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIBaQ&c=j5oPpO0eBH1iio48DtsedeEl > Zfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=7W > Fp_sPsfcSMHAePbKGxGBycZV5ThfIe8hNPvG7WqwY&s=SSPamQlUriyGMaTWlgoS4pwctC > qc39W4dGGLloesVyw&e= Post images on > https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIBaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=7WFp_sPsfcSMHAePbKGxGBycZV5ThfIe8hNPvG7WqwY&s=H3cSBvdDft_Ax5MWoB5oOddWtGcc5YroRzTh3U8rToY&e= and include the link in your posting. > ***** > > > We are submitting a request for a new lab which requires reporting > heat load in BTU/hr or watts to attempt proper air handling. > > We have a few forced air incubation chambers such as the > Weatherstation, Zeiss, and other large boxes that surround the > microscope stage. Does anyone know what are what are acceptable, > reasonable, or accurate numbers for 37 degrees C? The boxes are minimally insulated. > > Also, what are people reporting for metal halide lamps (such as X-Cite > or Leica EL6000)? (For HBO 100 back in the 1990s we said 1250 BTU/hr, > but I don't know where this number came from.) > > Any help appreciated. > > Thank you! > > > Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory NYU > Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY > 10016 > Office: 646-501-0567 Cell: 914-309-3270 [hidden email] > <mailto:[hidden email]> > https://urldefense.proofpoint.com/v2/url?u=http-3A__nyulmc.org_micros&d=DwIBaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=7WFp_sPsfcSMHAePbKGxGBycZV5ThfIe8hNPvG7WqwY&s=ay2AipeO-P4Vmf_JdMkUvMC8usnvO7knvWwrQT3a9F0&e= https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopynotes.com_&d=DwIBaQ&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=7WFp_sPsfcSMHAePbKGxGBycZV5ThfIe8hNPvG7WqwY&s=au6Mu8XKY99EV_CuTNVJuYYZeNPNiwAyBqPpTFHK1VY&e= > Acknowledgement in your publications and presentations of work > performed in the Microscopy Core plays a vital role in securing > support and the funding necessary to maintain and operate this valuable research resource. > For publications that were made possible by work performed in the > core, please use the acknowledgement statement "We thank the NYU > Langone Microscopy Core for experimental and technical support" and > include required grant numbers as listed here > https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopynotes.co > m_ilabnyu_acknowledgements2017.pdf&d=DwIBaQ&c=j5oPpO0eBH1iio48DtsedeEl > Zfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=7W > Fp_sPsfcSMHAePbKGxGBycZV5ThfIe8hNPvG7WqwY&s=PBJoaRImlz8KkWpxzEGekOIJFH > l83Xuh7VyGwr6DQO8&e= Please also consider staff for co-authorship if > they played a key role in the study. > -- -- Zdenek Svindrych, Ph.D. Research Associate - Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth |
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