help with Zeiss Observer Z1

[mcammer]

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We have a Zeiss Observer Z1 microscope body on our 710 confocal.

When we switch to the ocular setting the prism on the inside bottom of the microscope slides into position correctly to aim the light to the eyepieces or front camera port, but we cannot see anything through the oculars.  All the other electronic controlled motor positions appear to be working ok.

The touchpad does not draw a yellow line to the eye icon.

Does anyone have an idea how to fix this or a detailed diagram of the Observer innards?

Thank you!

________________________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

[Christina Polumbo]

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Hi Michael,

There is a physical switch under the oculars that can block the light regardless of where you send it in the light path. Is it possible that you may have switched that to the wrong position? It has a diagram of an eye with a line through it.

________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Cammer, Michael [[hidden email]]
Sent: Monday, August 20, 2012 12:10 PM
To: [hidden email]
Subject: help with Zeiss Observer Z1

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We have a Zeiss Observer Z1 microscope body on our 710 confocal.

When we switch to the ocular setting the prism on the inside bottom of the microscope slides into position correctly to aim the light to the eyepieces or front camera port, but we cannot see anything through the oculars.  All the other electronic controlled motor positions appear to be working ok.

The touchpad does not draw a yellow line to the eye icon.

Does anyone have an idea how to fix this or a detailed diagram of the Observer innards?

Thank you!

________________________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: [phone]  Cell: [phone]

[Vitaly Boyko]

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Dear All,

We are looking into white light laser for confocal system.

Could you, please share your experience with the white light laser including its performance in FLIM applications. Is 488 nm line strong enough? Is there a white light laser with broad spectral coverage that would also include 400-450 nm range?

Many thanks in advance,

Vitaly
240-342-6217

[Cameron, Lisa]

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We have a white light laser on our Leica SP5X confocal system which spans the range of 470nm-670nm.
It has worked well for us for regular confocal samples and applications, but we have not done FLIM on this system.
I am not sure of the model - it is supplied by Leica (no choice) - our system was purchased in Sept 2008.
We've had a replacement laser since, that has been working fine for us (same model as the original as far as I know).
The max laser power at 488nm on the WLL is about 1/5th the max laser power of our 488 line on our Argon laser at last measurement. Feel free to contact me if you have further questions -
Lisa


---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy Core
Dana Farber Cancer Institute
Boston, MA
http://microscopy.dfci.harvard.edu
617-582-8824

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vitaly Boyko
Sent: Monday, August 20, 2012 3:45 PM
To: [hidden email]
Subject: [CONFOCALMICROSCOPY] white light laser

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Dear All,

We are looking into white light laser for confocal system.

Could you, please share your experience with the white light laser including its performance in FLIM applications. Is 488 nm line strong enough? Is there a white light laser with broad spectral coverage that would also include 400-450 nm range?

Many thanks in advance,

Vitaly
240-342-6217


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.

[mcammer]

Thanks to the responses we got here and help from Zeiss over the phone we identified the problem and figured out a manual fix until the replacement part is shipped.  The problem is a shutter sticking in the barrel to the eyepieces which causes a safety interlock to stay open.  A gentle tug on fishline taped to the shutter closes it and the lasers switch on.
Regards,
Michael

From: Pedro Almada [mailto:[hidden email]]
Sent: Monday, August 20, 2012 12:41 PM
To: Cammer, Michael
Subject: Re: help with Zeiss Observer Z1

Hi Michael,

We have a 510 with an AxioVert 200 body and had a similar issue, no error messages on the logs. There is a safety shutter on the binocular head and it broke down. It was easy to replace it. We tried removing it completely but there is an interlock that prevents imaging without it. We just unscrewed the shutter and had it laying on the floor of the inside of the binocular so we could image until the service rep replaced the shutter. It's quite easy to unscrew and have a look, see if it's moving.

Best of luck,
Pedro

Research and Microscopy Technician
Unidade de Imagiologia Celular,
Instituto Gulbenkian de Ciência
Rua da Quinta Grande, 6
2780-156 Oeiras

Phone: + 351 214 464 607<tel:%2B%20351%20214%20464%20607>
Ext: 607

[Periasamy, Ammasi (ap3t)]

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I do have the SP5X, a white light laser system with hybrid detector.
You can use the white light laser for FLIM, the white light laser is a pulsed laser. But you need a detector to couple. You can use the Becker & Hickl or PicoQuant FLIM system and can be synchronized with the Leica scanning system. The FLIM company people will provide a hardware and software to collect the FLIM images with the Leica system.
The Leica company told me that they are working on a hybrid detector which can be used for FLIM too. DO not know when that detector will be ready for FLIM imaging in the SP5X system!!

Dr. Ammasi Periasamy
Professor & Center Director
W.M. Keck Center for Cellular Imaging (KCCI)
Biology, University of Virginia
B005, Physical Life Sciences Building (PLSB)
90, Geldard Drive, Charlottesville, VA 22904
(Campus Mail - P.O. Box 400328)
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:[phone]; Email:[hidden email]
http://www.kcci.virginia.edu/contact/peri.php
************************
12th Annual Workshop on FRET Microscopy, March 11-16, 2013
http://www.kcci.virginia.edu/workshop/workshop2013/index.php
*************************


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cameron, Lisa
Sent: Monday, August 20, 2012 4:29 PM
To: [hidden email]
Subject: Re: white light laser

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To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

We have a white light laser on our Leica SP5X confocal system which spans the range of 470nm-670nm.
It has worked well for us for regular confocal samples and applications, but we have not done FLIM on this system.
I am not sure of the model - it is supplied by Leica (no choice) - our system was purchased in Sept 2008.
We've had a replacement laser since, that has been working fine for us (same model as the original as far as I know).
The max laser power at 488nm on the WLL is about 1/5th the max laser power of our 488 line on our Argon laser at last measurement. Feel free to contact me if you have further questions - Lisa


---------------------------------------
Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy Core Dana Farber Cancer Institute Boston, MA http://microscopy.dfci.harvard.edu
617-582-8824

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Vitaly Boyko
Sent: Monday, August 20, 2012 3:45 PM
To: [hidden email]
Subject: [CONFOCALMICROSCOPY] white light laser

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear All,

We are looking into white light laser for confocal system.

Could you, please share your experience with the white light laser including its performance in FLIM applications. Is 488 nm line strong enough? Is there a white light laser with broad spectral coverage that would also include 400-450 nm range?

Many thanks in advance,

Vitaly
240-342-6217


The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.

[Craig Brideau]

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Most of the continuum lasers don't emit shorter than ~450nm, so to get as
short as 400 might be troublesome.

Craig


On Mon, Aug 20, 2012 at 3:53 PM, Periasamy, Ammasi (ap3t) <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I do have the SP5X, a white light laser system with hybrid detector.
> You can use the white light laser for FLIM, the white light laser is a
> pulsed laser. But you need a detector to couple. You can use the Becker &
> Hickl or PicoQuant FLIM system and can be synchronized with the Leica
> scanning system. The FLIM company people will provide a hardware and
> software to collect the FLIM images with the Leica system.
> The Leica company told me that they are working on a hybrid detector which
> can be used for FLIM too. DO not know when that detector will be ready for
> FLIM imaging in the SP5X system!!
>
> Dr. Ammasi Periasamy
> Professor & Center Director
> W.M. Keck Center for Cellular Imaging (KCCI)
> Biology, University of Virginia
> B005, Physical Life Sciences Building (PLSB)
> 90, Geldard Drive, Charlottesville, VA 22904
> (Campus Mail - P.O. Box 400328)
> Voice: 434-243-7602 (Office); 982-4869 (lab)
> Fax:[phone]; Email:[hidden email]
> http://www.kcci.virginia.edu/contact/peri.php
> ************************
> 12th Annual Workshop on FRET Microscopy, March 11-16, 2013
> http://www.kcci.virginia.edu/workshop/workshop2013/index.php
> *************************
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Cameron, Lisa
> Sent: Monday, August 20, 2012 4:29 PM
> To: [hidden email]
> Subject: Re: white light laser
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We have a white light laser on our Leica SP5X confocal system which spans
> the range of 470nm-670nm.
> It has worked well for us for regular confocal samples and applications,
> but we have not done FLIM on this system.
> I am not sure of the model - it is supplied by Leica (no choice) - our
> system was purchased in Sept 2008.
> We've had a replacement laser since, that has been working fine for us
> (same model as the original as far as I know).
> The max laser power at 488nm on the WLL is about 1/5th the max laser power
> of our 488 line on our Argon laser at last measurement. Feel free to
> contact me if you have further questions - Lisa
>
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy Core Dana Farber Cancer
> Institute Boston, MA http://microscopy.dfci.harvard.edu
> 617-582-8824
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Vitaly Boyko
> Sent: Monday, August 20, 2012 3:45 PM
> To: [hidden email]
> Subject: [CONFOCALMICROSCOPY] white light laser
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
>
> We are looking into white light laser for confocal system.
>
> Could you, please share your experience with the white light laser
> including its performance in FLIM applications. Is 488 nm line strong
> enough? Is there a white light laser with broad spectral coverage that
> would also include 400-450 nm range?
>
> Many thanks in advance,
>
> Vitaly
> 240-342-6217
>
>
> The information in this e-mail is intended only for the person to whom it
> is addressed. If you believe this e-mail was sent to you in error and the
> e-mail contains patient information, please contact the Partners Compliance
> HelpLine at http://www.partners.org/complianceline . If the e-mail was
> sent to you in error but does not contain patient information, please
> contact the sender and properly dispose of the e-mail.
>

[Periasamy, Ammasi (ap3t)]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Try this company, they have all kinds of pulsed lasers if you wanted to build on your own a FLIM system
http://markettechinc.net/Products/Femtosecond_Lasers.aspx
good luck


Dr. Ammasi Periasamy
Professor & Center Director
W.M. Keck Center for Cellular Imaging (KCCI)
Biology, University of Virginia
B005, Physical Life Sciences Building (PLSB)
90, Geldard Drive, Charlottesville, VA 22904
(Campus Mail - P.O. Box 400328)
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:[phone]; Email:[hidden email]
http://www.kcci.virginia.edu/contact/peri.php
************************
12th Annual Workshop on FRET Microscopy, March 11-16, 2013
http://www.kcci.virginia.edu/workshop/workshop2013/index.php
*************************

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau
Sent: Monday, August 27, 2012 10:04 PM
To: [hidden email]
Subject: Re: white light laser

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Most of the continuum lasers don't emit shorter than ~450nm, so to get as short as 400 might be troublesome.

Craig


On Mon, Aug 20, 2012 at 3:53 PM, Periasamy, Ammasi (ap3t) < [hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I do have the SP5X, a white light laser system with hybrid detector.
> You can use the white light laser for FLIM, the white light laser is a
> pulsed laser. But you need a detector to couple. You can use the
> Becker & Hickl or PicoQuant FLIM system and can be synchronized with
> the Leica scanning system. The FLIM company people will provide a
> hardware and software to collect the FLIM images with the Leica system.
> The Leica company told me that they are working on a hybrid detector
> which can be used for FLIM too. DO not know when that detector will be
> ready for FLIM imaging in the SP5X system!!
>
> Dr. Ammasi Periasamy
> Professor & Center Director
> W.M. Keck Center for Cellular Imaging (KCCI) Biology, University of
> Virginia B005, Physical Life Sciences Building (PLSB) 90, Geldard
> Drive, Charlottesville, VA 22904 (Campus Mail - P.O. Box 400328)
> Voice: 434-243-7602 (Office); 982-4869 (lab) Fax:[phone];
> Email:[hidden email] http://www.kcci.virginia.edu/contact/peri.php
> ************************
> 12th Annual Workshop on FRET Microscopy, March 11-16, 2013
> http://www.kcci.virginia.edu/workshop/workshop2013/index.php
> *************************
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Cameron, Lisa
> Sent: Monday, August 20, 2012 4:29 PM
> To: [hidden email]
> Subject: Re: white light laser
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We have a white light laser on our Leica SP5X confocal system which spans
> the range of 470nm-670nm.
> It has worked well for us for regular confocal samples and applications,
> but we have not done FLIM on this system.
> I am not sure of the model - it is supplied by Leica (no choice) - our
> system was purchased in Sept 2008.
> We've had a replacement laser since, that has been working fine for us
> (same model as the original as far as I know).
> The max laser power at 488nm on the WLL is about 1/5th the max laser power
> of our 488 line on our Argon laser at last measurement. Feel free to
> contact me if you have further questions - Lisa
>
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy Core Dana Farber Cancer
> Institute Boston, MA http://microscopy.dfci.harvard.edu
> 617-582-8824
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Vitaly Boyko
> Sent: Monday, August 20, 2012 3:45 PM
> To: [hidden email]
> Subject: [CONFOCALMICROSCOPY] white light laser
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
>
> We are looking into white light laser for confocal system.
>
> Could you, please share your experience with the white light laser
> including its performance in FLIM applications. Is 488 nm line strong
> enough? Is there a white light laser with broad spectral coverage that
> would also include 400-450 nm range?
>
> Many thanks in advance,
>
> Vitaly
> 240-342-6217
>
>
> The information in this e-mail is intended only for the person to whom it
> is addressed. If you believe this e-mail was sent to you in error and the
> e-mail contains patient information, please contact the Partners Compliance
> HelpLine at http://www.partners.org/complianceline . If the e-mail was
> sent to you in error but does not contain patient information, please
> contact the sender and properly dispose of the e-mail.
>