image pro plus and 2d/3d Tracking ?

[Jean-Pierre CLAMME-2]

Hi,

I was wondering if anyone used Image pro plus for 2D and 3D particule
tracking in cells ? If yes how does it perform ?
Thanks

JP

[Armstrong, Brian]

JP. Image Pro has powerful tools for doing this. The answer with regards
to performance; it depends, it depends, and it depends.
There are different methods for doing this and you need to work with
them in order to find a solution that yields the best performance for
your sample.

You can always do it manually by clicking on the cells, Image Pro
automatically advances through the Z-stack presenting the next optical
slice.  
The automated counting methods will take some time to perfect.  

Brian Armstrong PhD
Manager, Light Microscopy Core
Beckman Research Institute
1450 East Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/SharedResources/LightMicroscopy/LightMicroHome
.htm
 
 
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Jean-Pierre CLAMME
Sent: Monday, January 26, 2009 11:25 AM
To: [hidden email]
Subject: image pro plus and 2d/3d Tracking ?

Hi,

I was wondering if anyone used Image pro plus for 2D and 3D particule
tracking in cells ? If yes how does it perform ?
Thanks

JP


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[Jean-Pierre CLAMME-2]

Hi,

I was used to work with volocity in my previous lab, but for here, I'm looking for something more polyvalent that can also do tracking. So I thought image pro was a good choice.

Thank you for the information

JP


- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Jean-Pierre CLAMME, PhD
Senior Scientist
Nitto Denko Technical
501 Via Del Monte
Oceanside, CA 92058
E-mail: [hidden email]
Phone: [phone]


Confocal Microscopy List <[hidden email]> wrote on 01/26/2009 11:47:56 AM:

> JP. Image Pro has powerful tools for doing this. The answer with regards
> to performance; it depends, it depends, and it depends.
> There are different methods for doing this and you need to work with
> them in order to find a solution that yields the best performance for
> your sample.

> You can always do it manually by clicking on the cells, Image Pro
> automatically advances through the Z-stack presenting the next optical
> slice.
> The automated counting methods will take some time to perfect.

> Brian Armstrong PhD
> Manager, Light Microscopy Core
> Beckman Research Institute
> 1450 East Duarte Rd
> Duarte, CA 91010
> 626-256-4673 x62872
> http://www.cityofhope.org/SharedResources/LightMicroscopy/LightMicroHome
> .htm

>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Jean-Pierre CLAMME
> Sent: Monday, January 26, 2009 11:25 AM
> To: [hidden email]
> Subject: image pro plus and 2d/3d Tracking ?

> Hi,

> I was wondering if anyone used Image pro plus for 2D and 3D particule
> tracking in cells ? If yes how does it perform ?
> Thanks

> JP

>
> ---------------------------------------------------------------------

> SECURITY/CONFIDENTIALITY WARNING:
> This message and any attachments are intended solely for the
> individual or entity to which they are addressed. This communication
> may contain information that is privileged, confidential, or exempt
> from disclosure under applicable law (e.g., personal health
> information, research data, financial information). Because this e-
> mail has been sent without encryption, individuals other than the
> intended recipient may be able to view the information, forward it
> to others or tamper with the information without the knowledge or
> consent of the sender. If you are not the intended recipient, or the
> employee or person responsible for delivering the message to the
> intended recipient, any dissemination, distribution or copying of
> the communication is strictly prohibited. If you received the
> communication in error, please notify the sender immediately by
> replying to this message and deleting the message and any
> accompanying files from your system. If, due to the security risks,
> you do not wish to receive further communications via e-mail, please
> reply to this message and inform the sender that you do not wish to
> receive further e-mail from the sender.
> ---------------------------------------------------------------------

[Olivier Bardot]

Hello
Have you ever tried Imaris from Bitplane?
It seems to me that it will fulfill your needs.
They have an 7-days evaluation software.
Olivier