immersion oil
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi All I would like some advice on immersion oils. I have used Cargille in the past and their LDF type has the correct RI but they claim it has an odor. Has anyone used it and found it unpleasant? They also have type HF but it has a high viscosity (700 cSt) and may be problematic if the cover slip is not attached firmly to the sample. Any comments? I do like the ease of use of their 7.4cc dispensers. I have used both Zeiss oil on a Zeiss confocal and Leica oil on a Leica confocal and they have worked well. Any other suggestions? Thanks Judy Trogadis Bio-Imaging Coordinator Keenan Research Centre, St. Michael's Hospital 209 Victoria Street Toronto M5B 1T8, Canada office: 416-864-6060 ext. 77612 imaging facility: ext. 77434 cell: 416-254-9330 [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We actually just ran into this problem at out lab as well. We found that the cargille has a strong moth ball like odor. The general consensus was that we could not deal with the odor. The closest oil that cargille carries with out the odor has background fluorescence. After our test (with confocal and widefield / on multiple brand microscope (zeiss, olympus, and leica) ) we found that the zeiss oil has the best performance overall for all of the microscope. We did not have leica oil to test. We tested the delta vision, zeiss, olympus, and two cargille oils. Hope this helps. |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thank you, that's very helpful. I wonder whether Cargille is reading this posting :-) Judy -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Hillary Guzik Sent: Thursday, July 03, 2014 11:36 AM To: [hidden email] Subject: Re: immersion oil ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We actually just ran into this problem at out lab as well. We found that the cargille has a strong moth ball like odor. The general consensus was that we could not deal with the odor. The closest oil that cargille carries with out the odor has background fluorescence. After our test (with confocal and widefield / on multiple brand microscope (zeiss, olympus, and leica) ) we found that the zeiss oil has the best performance overall for all of the microscope. We did not have leica oil to test. We tested the delta vision, zeiss, olympus, and two cargille oils. Hope this helps. |
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In reply to this post by Judy Trogadis-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Because Zeiss and Leica use the same oil to my knowledge, it is not unexpected to find both working equally well. Best regards, Jens http://br.linkedin.com/pub/jens-rietdorf/6/4a3/189/ Skype jens.Rietdorf Am 03.07.2014 12:04 schrieb "Judy Trogadis" <[hidden email]>: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi All > > I would like some advice on immersion oils. I have used Cargille in the > past and > their LDF type has the correct RI but they claim it has an odor. Has anyone > used it and found it unpleasant? They also have type HF but it has a high > viscosity (700 cSt) and may be problematic if the cover slip is not > attached > firmly to the sample. Any comments? I do like the ease of use of their > 7.4cc > dispensers. > > I have used both Zeiss oil on a Zeiss confocal and Leica oil on a Leica > confocal > and they have worked well. > > Any other suggestions? > > Thanks > > > Judy Trogadis > Bio-Imaging Coordinator > Keenan Research Centre, St. Michael's Hospital > 209 Victoria Street > Toronto M5B 1T8, Canada > office: 416-864-6060 ext. 77612 > imaging facility: ext. 77434 > cell: 416-254-9330 > [hidden email] > |
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In reply to this post by Judy Trogadis-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Yes, the odor was quite unpleasant. Cargille reformulated their oils. We were able to obtain samples from them for testing. We now routinely use Cargille type HF (Formula code 368) for most of our applications. and Cargille type A (Formula code 1248) for applications where low viscosity is needed. This is primarily for live cell imaging with fast z acquisition where the type HF creates too much drag. Type A is quite runny, and special care is needed on inverted scopes. The Zeiss immersol 518 works fine too. It is a bit less viscous than Cargille HF. Julio Vazquez Fred Hutchinson Cancer Research Center Seattle, WA 98109 On Jul 3, 2014, at 8:03 AM, Judy Trogadis wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi All > > I would like some advice on immersion oils. I have used Cargille in the past and > their LDF type has the correct RI but they claim it has an odor. Has anyone > used it and found it unpleasant? They also have type HF but it has a high > viscosity (700 cSt) and may be problematic if the cover slip is not attached > firmly to the sample. Any comments? I do like the ease of use of their 7.4cc > dispensers. > > I have used both Zeiss oil on a Zeiss confocal and Leica oil on a Leica confocal > and they have worked well. > > Any other suggestions? > > Thanks > > > Judy Trogadis > Bio-Imaging Coordinator > Keenan Research Centre, St. Michael's Hospital > 209 Victoria Street > Toronto M5B 1T8, Canada > office: 416-864-6060 ext. 77612 > imaging facility: ext. 77434 > cell: 416-254-9330 > [hidden email] |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Wow, my own stash of Cargille oil (Type A, B, and 37) has no scent at all. I bought it about a year ago. Have they reformulated since then? Craig Brideau On Thu, Jul 3, 2014 at 10:39 AM, Julio Vazquez <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > > Yes, the odor was quite unpleasant. Cargille reformulated their oils. We > were able to obtain samples from them for testing. We now routinely use > Cargille type HF (Formula code 368) for most of our applications. and > Cargille type A (Formula code 1248) for applications where low viscosity is > needed. This is primarily for live cell imaging with fast z acquisition > where the type HF creates too much drag. Type A is quite runny, and special > care is needed on inverted scopes. The Zeiss immersol 518 works fine too. > It is a bit less viscous than Cargille HF. > > Julio Vazquez > Fred Hutchinson Cancer Research Center > Seattle, WA 98109 > > > > On Jul 3, 2014, at 8:03 AM, Judy Trogadis wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hi All > > > > I would like some advice on immersion oils. I have used Cargille in the > past and > > their LDF type has the correct RI but they claim it has an odor. Has > anyone > > used it and found it unpleasant? They also have type HF but it has a high > > viscosity (700 cSt) and may be problematic if the cover slip is not > attached > > firmly to the sample. Any comments? I do like the ease of use of their > 7.4cc > > dispensers. > > > > I have used both Zeiss oil on a Zeiss confocal and Leica oil on a Leica > confocal > > and they have worked well. > > > > Any other suggestions? > > > > Thanks > > > > > > Judy Trogadis > > Bio-Imaging Coordinator > > Keenan Research Centre, St. Michael's Hospital > > 209 Victoria Street > > Toronto M5B 1T8, Canada > > office: 416-864-6060 ext. 77612 > > imaging facility: ext. 77434 > > cell: 416-254-9330 > > [hidden email] > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Don't remember the exact details, but I think Cargille discontinued the immersion oil type DF that we had been using for years in early 2011. For a while, DF was not available. Then they came up with a new formulation that was supposed to be a replacement for DF, but had the nasty smell. In the following months, we purchased small amounts of various Cargille oils (not sure if these were new or old products), until we settled on the type HF and type A. Julio Vazquez Fred Hutchinson Cancer Research Center Seattle, WA 98109 On Jul 3, 2014, at 11:59 AM, Craig Brideau wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Wow, my own stash of Cargille oil (Type A, B, and 37) has no scent at all. > I bought it about a year ago. Have they reformulated since then? > > Craig Brideau |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Do the oils other than DF have a strong odor? Craig On Thu, Jul 3, 2014 at 2:36 PM, Julio Vazquez <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Don't remember the exact details, but I think Cargille discontinued the > immersion oil type DF that we had been using for years in early 2011. For a > while, DF was not available. Then they came up with a new formulation that > was supposed to be a replacement for DF, but had the nasty smell. In the > following months, we purchased small amounts of various Cargille oils (not > sure if these were new or old products), until we settled on the type HF > and type A. > > > Julio Vazquez > Fred Hutchinson Cancer Research Center > Seattle, WA 98109 > > > > > > > On Jul 3, 2014, at 11:59 AM, Craig Brideau wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Wow, my own stash of Cargille oil (Type A, B, and 37) has no scent at > all. > > I bought it about a year ago. Have they reformulated since then? > > > > Craig Brideau > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** No.. the HF and A oils we are currently using don't have the odor. The original DF oil was Cat # 16242. This was replaced by DF Cat # 48218-506. I think this was the stinky one. Then the DF disappeared from the Cargille product list. I don't know if any of the other oils currently listed have the odor problem. If you are interested, our imaging specialist Dave McDonald can send you more info when he comes back from his vacation... he tested quite a few oils, and still has samples around. You may also be able to get the info from Cargille. Julio On Jul 3, 2014, at 1:50 PM, Craig Brideau wrote: > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Do the oils other than DF have a strong odor? > > Craig |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Cargille told me that they replaced the DF oil with the LDF and that one has the smell. Judy -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Julio Vazquez Sent: Thursday, July 03, 2014 5:05 PM To: [hidden email] Subject: Re: immersion oil ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** No.. the HF and A oils we are currently using don't have the odor. The original DF oil was Cat # 16242. This was replaced by DF Cat # 48218-506. I think this was the stinky one. Then the DF disappeared from the Cargille product list. I don't know if any of the other oils currently listed have the odor problem. If you are interested, our imaging specialist Dave McDonald can send you more info when he comes back from his vacation... he tested quite a few oils, and still has samples around. You may also be able to get the info from Cargille. Julio On Jul 3, 2014, at 1:50 PM, Craig Brideau wrote: > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Do the oils other than DF have a strong odor? > > Craig |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thanks for the tips! I bought quite a bit of oil so I should be good with my existing stock for quite a while. When I run out I will make sure to check for any formulation changes before I buy a new supply! Craig On Thu, Jul 3, 2014 at 3:09 PM, Judy Trogadis <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Cargille told me that they replaced the DF oil with the LDF and that one > has the smell. > > Judy > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of Julio Vazquez > Sent: Thursday, July 03, 2014 5:05 PM > To: [hidden email] > Subject: Re: immersion oil > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > No.. the HF and A oils we are currently using don't have the odor. The > original DF oil was Cat # 16242. This was replaced by DF Cat # 48218-506. I > think this was the stinky one. Then the DF disappeared from the Cargille > product list. I don't know if any of the other oils currently listed have > the odor problem. If you are interested, our imaging specialist Dave > McDonald can send you more info when he comes back from his vacation... he > tested quite a few oils, and still has samples around. You may also be able > to get the info from Cargille. > > > Julio > > > > > > On Jul 3, 2014, at 1:50 PM, Craig Brideau wrote: > > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Do the oils other than DF have a strong odor? > > > > Craig > |
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