intracellular ion probes

[mmodel]

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Dear All,

Intracellular ion probes behave differently in the cytoplasm than in the buffer - that is well known. I recall that I read somewhere that this difference is because of viscosity or because of interactions with proteins or maybe because of something else - I got an impression that someone has studied it. But I don't remember where I saw this and cannot think of the right keyword to find the information again. I would be very thankful if someone points me in the right direction.

Mike Model

[Mark Cannell-2]

***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Mike

I know Steve Baylor (Penn) did quite a lot of work in this area e.g. see

Properties of tri- and tetracarboxylate Ca2+ indicators in frog skeletal muscle fibers, 1996. Properties of tri- and tetracarboxylate Ca2+ indicators in frog skeletal muscle fibers 70, 896–916. doi:10.1016/S0006-3495(96)79633-9

HTH Mark

On 28/09/2016, at 1:45 am, MODEL, MICHAEL <[hidden email]> wrote:

***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. *****

Dear All,

Intracellular ion probes behave differently in the cytoplasm than in the buffer - that is well known. I recall that I read somewhere that this difference is because of viscosity or because of interactions with proteins or maybe because of something else - I got an impression that someone has studied it. But I don't remember where I saw this and cannot think of the right keyword to find the information again. I would be very thankful if someone points me in the right direction.

Mike Model

Mark  B. Cannell Ph.D. FRSNZ FISHR
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Faculty of Biomedical Sciences
University of Bristol
Bristol
BS8 1TD UK

[hidden email]

[Guillermo Marques]

***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** You may want to look into Amy Palmer’s work on metal sensors in cell organelles.  

https://palmerlab.colorado.edu

Guillermo Marqués
University Imaging Centers
University of Minnesota
Jackson Hall 1-151 
321 Church St SE
Minneapolis, MN55455

On Sep 27, 2016, at 7:45 PM, MODEL, MICHAEL <[hidden email]> wrote:

***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. *****

Dear All,

Intracellular ion probes behave differently in the cytoplasm than in the buffer - that is well known. I recall that I read somewhere that this difference is because of viscosity or because of interactions with proteins or maybe because of something else - I got an impression that someone has studied it. But I don't remember where I saw this and cannot think of the right keyword to find the information again. I would be very thankful if someone points me in the right direction.

Mike Model