live animal imaging question

[mcammer]

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We are wondering what methods people are using to keep mice or other small animals warm during extended imaging sessions under anesthesia.

Are people using chambers that completely surround the microscope or other solutions such as heated pads?  Any help, including manufacturers and part numbers if using something other than large chambers, would be very much appreciated.

Thanks!

________________________________________________________
Michael Cammer, Assistant Research Scientist
Microscopy Core, NYU Langone Medical Center & Skirball Institute of Biomolecular Medicine
Cell: [phone]   Microscopy Lab: [phone]   Dustin Lab: [phone]

[Romin, Yevgeniy/Sloan Kettering Institute]

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Some of our users use kind of a heating fan that is put next to the system and blows air of a specific temperature on to the stage.  I can look into manufacturer/part number for you if this sounds like something you may be interested in.

Yevgeniy





Jul 1, 2013, в 2:18 PM, "Cammer, Michael" <[hidden email]> написал(а):

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We are wondering what methods people are using to keep mice or other small animals warm during extended imaging sessions under anesthesia.
>
> Are people using chambers that completely surround the microscope or other solutions such as heated pads?  Any help, including manufacturers and part numbers if using something other than large chambers, would be very much appreciated.
>
> Thanks!
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist
> Microscopy Core, NYU Langone Medical Center & Skirball Institute of Biomolecular Medicine
> Cell: [phone]   Microscopy Lab: [phone]   Dustin Lab: [phone]
>

 
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[Steve Alexander]

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We are using heating plates from Tokaihit or Linkam. But the Tokaihit are pretty expensive.

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Yevgeniy Romin
Sent: Monday, July 01, 2013 1:48 PM
To: [hidden email]
Subject: Re: live animal imaging question

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Some of our users use kind of a heating fan that is put next to the system and blows air of a specific temperature on to the stage.  I can look into manufacturer/part number for you if this sounds like something you may be interested in.

Yevgeniy





Jul 1, 2013, в 2:18 PM, "Cammer, Michael" <[hidden email]> написал(а):

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We are wondering what methods people are using to keep mice or other small animals warm during extended imaging sessions under anesthesia.
>
> Are people using chambers that completely surround the microscope or other solutions such as heated pads?  Any help, including manufacturers and part numbers if using something other than large chambers, would be very much appreciated.
>
> Thanks!
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist Microscopy Core, NYU
> Langone Medical Center & Skirball Institute of Biomolecular Medicine
> Cell: [phone]   Microscopy Lab: [phone]   Dustin Lab: [phone]
>

 
     =====================================================================
     
     Please note that this e-mail and any files transmitted from
     Memorial Sloan-Kettering Cancer Center may be privileged, confidential,
     and protected from disclosure under applicable law. If the reader of
     this message is not the intended recipient, or an employee or agent
     responsible for delivering this message to the intended recipient,
     you are hereby notified that any reading, dissemination, distribution,
     copying, or other use of this communication or any of its attachments
     is strictly prohibited.  If you have received this communication in
     error, please notify the sender immediately by replying to this message
     and deleting this message, any attachments, and all copies and backups
     from your computer.

[Julio Vazquez]

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A methods paper (Ewald, Werb and Egeblat: Preparation of mice for long-term intravital imaging of the mammary gland; CSH Protocols 2011) mentions heating lamps and/or heating blankets.

Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA 98109

http://www.fhcrc.org/en.html

==

On Jul 1, 2013, at 11:17 AM, Cammer, Michael wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We are wondering what methods people are using to keep mice or other small animals warm during extended imaging sessions under anesthesia.
>
> Are people using chambers that completely surround the microscope or other solutions such as heated pads?  Any help, including manufacturers and part numbers if using something other than large chambers, would be very much appreciated.
>
> Thanks!
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist
> Microscopy Core, NYU Langone Medical Center & Skirball Institute of Biomolecular Medicine
> Cell: [phone]   Microscopy Lab: [phone]   Dustin Lab: [phone]

[Hana Uhlirova]

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We are using heating blankets with a feedback rectal probe from Harvard
Apparatus. They work very well..

http://www.harvardapparatus.com/webapp/wcs/stores/servlet/haisku1_10001_11051_39115_-1_hai_ProductDetail_N_37610

Hana
--------------------------------------------------------------------------------

Hana Uhlirova

Postdoctoral Fellow
Laboratory of Neurovascular Imaging
Department of Radiology
Medical Teaching Facility, Room 353

9500 Gilman Dr., MC 0624
La Jolla CA 92093

Tel: [phone]

----- Original Message -----
From: "Cammer, Michael" <[hidden email]>
To: <[hidden email]>
Sent: Monday, July 01, 2013 11:17 AM
Subject: live animal imaging question


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We are wondering what methods people are using to keep mice or other small
animals warm during extended imaging sessions under anesthesia.

Are people using chambers that completely surround the microscope or other
solutions such as heated pads?  Any help, including manufacturers and part
numbers if using something other than large chambers, would be very much
appreciated.

Thanks!

________________________________________________________
Michael Cammer, Assistant Research Scientist
Microscopy Core, NYU Langone Medical Center & Skirball Institute of
Biomolecular Medicine
Cell: [phone]   Microscopy Lab: [phone]   Dustin Lab: (212)
263-3208

[Pascal Weber]

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I worked on mice 2P for several hours without it is cold. I prepared a heating
system with electric resistors for electrical cabinet thermostat and precise probe
of 1/100 of a degree. All purchased from RS. I crafted it for a room around an
upright microscope and installed more fans to increase and homogenized flow of
hot air.
Something like that

http://radiospares-fr.rs-online.com/web/p/chauffages-de-boitier/6974395/

[Richard Cole]

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To get my animal protocol approved I had to have the following for long term
MP intravital imaging:

 

Restive heating pad below the animal, blanket on top, MouseOx monitoring
system and rectal temp probe w/audible alarm.

 

Hope that this helps.

 

Rich

 

 

Richard Cole
Research Scientist V
Director: Advanced Light Microscopy Core Unit
Wadsworth Center

 

Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York


P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-474-4430 Fax

 

Email  <mailto:[hidden email]> [hidden email]

Website  <http://www.wadsworth.org/cores/alm/index.htm>
www.wadsworth.org/cores/alm/index.htm

 

 

 

[mcammer]

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Thank you everyone who replied.  The answers have been extremely helpful!
Regards,
Michael

________________________________________________________
Michael Cammer, Assistant Research Scientist
Microscopy Core, NYU Langone Medical Center & Skirball Institute of
Biomolecular Medicine
Cell: [phone]   Microscopy Lab: [phone]   Dustin Lab: (212)
263-3208