looking for "beautiful" sample

[Alessandro Esposito]

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Dear all,
    here it is, a silly question. I wish to demonstrate the capabilities of
our new system that can read-out fluorescence lifetime, anisotropy and
spectra wih TPE. I have plenty of information-rich images, either images
from tissue or fluorescent proteins, but I would like to prepare an
eye-catching set of images.

I am a bit bored to use Convallaria... any suggestion for a fluorescent or
autofluorescent sample tha could provide a relative complex emission that
could look good?

Cheers,

Alessandro

[Coutu, Cathy]

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Hi Allesandro,

Commercial pre-stained plant tissues look nice and are usually quite fluorescent.
Also, pollen grains are autofluorescent and look cool in 3D.

Cathy

Cathy Coutu, M. Sc.
Technician / Technicienne
Genomics, Bioinformatics, and other Bioinformation / Génomique, Bioinformatique et Bioinformation
Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alessandro Esposito
Sent: October-01-11 4:10 AM
To: [hidden email]
Subject: looking for "beautiful" sample

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Dear all,
    here it is, a silly question. I wish to demonstrate the capabilities of
our new system that can read-out fluorescence lifetime, anisotropy and
spectra wih TPE. I have plenty of information-rich images, either images
from tissue or fluorescent proteins, but I would like to prepare an
eye-catching set of images.

I am a bit bored to use Convallaria... any suggestion for a fluorescent or
autofluorescent sample tha could provide a relative complex emission that
could look good?

Cheers,

Alessandro

[Jean-Yves Tinevez-3]

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>
>
> Dear all,
>    here it is, a silly question. I wish to demonstrate the  
> capabilities of
> our new system that can read-out fluorescence lifetime, anisotropy and
> spectra wih TPE. I have plenty of information-rich images, either  
> images
> from tissue or fluorescent proteins, but I would like to prepare an
> eye-catching set of images.
>
> I am a bit bored to use Convallaria... any suggestion for a  
> fluorescent or
> autofluorescent sample tha could provide a relative complex emission  
> that
> could look good?

Hi Alessandro,

Bouncing on Cathy's advice, I strongly encourages you to try the  
pollen, though in my hemisphere, it is no longer the adequate season.  
They are autofluorescent in a very wide range of wavelengths, have  
awesome 3D structure. On top of that, meta-scientifically:
- It is a fantastic hobby to do with your kids: go out with them with  
slides, coverslips, oil and nail-polish and go collect pollen from the  
flowers of your garden
- Since it is not the season, it is a good opportunity to offer  
flowers to your wife. In my case however, I did not date bringing the  
bill to the lab.

Best regards
jy



--
Jean-Yves Tinevez
PFID - Imagopole
Institut Pasteur
25-28, rue du Docteur Roux
75724 Paris cedex 15
France
tel: [phone]

[Carol Bayles]

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Even easier than pollen is a leaf.  Just grab one from any plant. Cut a small piece and put it under a coverslip.  Try to eliminate air pockets.  Plants have marvelous autofluorescence.
Carol

:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:=:
Carol Bayles, Manager
Cornell Imaging (Ci)
Life Sciences Core Lab Center
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607-254-4860
607-254-6379 fax
http://cores.lifesciences.cornell.edu
Nanobiotechnology Center (NBTC)
www.nbtc.cornell.edu
Cornell University
Ithaca NY 14853


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alessandro Esposito
Sent: Saturday, October 01, 2011 6:10 AM
To: [hidden email]
Subject: looking for "beautiful" sample

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Dear all,
    here it is, a silly question. I wish to demonstrate the capabilities of
our new system that can read-out fluorescence lifetime, anisotropy and
spectra wih TPE. I have plenty of information-rich images, either images
from tissue or fluorescent proteins, but I would like to prepare an
eye-catching set of images.

I am a bit bored to use Convallaria... any suggestion for a fluorescent or
autofluorescent sample tha could provide a relative complex emission that
could look good?

Cheers,

Alessandro

[Zac Arrac Atelaz]

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I suggest also plants, transversal cut of the stem will be amazing, and there are old school dyes that have differential fluorescence also.
 
Gabriel OH
 

> Date: Mon, 3 Oct 2011 16:35:22 +0200
> From: [hidden email]
> Subject: Re: looking for "beautiful" sample
> To: [hidden email]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> >
> >
> > Dear all,
> > here it is, a silly question. I wish to demonstrate the
> > capabilities of
> > our new system that can read-out fluorescence lifetime, anisotropy and
> > spectra wih TPE. I have plenty of information-rich images, either
> > images
> > from tissue or fluorescent proteins, but I would like to prepare an
> > eye-catching set of images.
> >
> > I am a bit bored to use Convallaria... any suggestion for a
> > fluorescent or
> > autofluorescent sample tha could provide a relative complex emission
> > that
> > could look good?
>
> Hi Alessandro,
>
> Bouncing on Cathy's advice, I strongly encourages you to try the
> pollen, though in my hemisphere, it is no longer the adequate season.
> They are autofluorescent in a very wide range of wavelengths, have
> awesome 3D structure. On top of that, meta-scientifically:
> - It is a fantastic hobby to do with your kids: go out with them with
> slides, coverslips, oil and nail-polish and go collect pollen from the
> flowers of your garden
> - Since it is not the season, it is a good opportunity to offer
> flowers to your wife. In my case however, I did not date bringing the
> bill to the lab.
>
> Best regards
> jy
>
>
>
> --
> Jean-Yves Tinevez
> PFID - Imagopole
> Institut Pasteur
> 25-28, rue du Docteur Roux
> 75724 Paris cedex 15
> France
> tel: [phone]

     

[Gregg Sobocinski]

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Alessandro,
I've been meaning to purchase something more interesting, myself. From a cost-value point of view, Carolina Biological has an excellent selection. I've been wanting to purchase a mixed pollen slide from them, but I didn't see it with my initial, quick look today.

http://www.carolina.com/category/30000/30800.do

Plants autofluoresce wonderfully. If you want to separate the channels, you'll want to be more specific, though. I've seen Lily anther's used to great effect.

When I train users on the confocal microscope, I use a Molecular Probes cell slide#2. At $130 it's pricey, but I get a DAPI, Bodipy, and Texas Red slide, which offers the challenges of triple-staining, making training that much more effective.

Good luck,
~Gregg

Gregg Sobocinski
Microscope Imaging Specialist
University of Michigan
Molecular, Cellular, and Developmental Biology
Ann Arbor, Michigan
USA


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alessandro Esposito
Sent: Saturday, October 01, 2011 6:10 AM
To: [hidden email]
Subject: looking for "beautiful" sample

*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear all,
    here it is, a silly question. I wish to demonstrate the capabilities of
our new system that can read-out fluorescence lifetime, anisotropy and
spectra wih TPE. I have plenty of information-rich images, either images
from tissue or fluorescent proteins, but I would like to prepare an
eye-catching set of images.

I am a bit bored to use Convallaria... any suggestion for a fluorescent or
autofluorescent sample tha could provide a relative complex emission that
could look good?

Cheers,

Alessandro

[Julia Edgar]

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Dear All
Can anybody recommend a good photo-switchable and/or photo-activatable reporter.
Thank you
Julia Edgar
University of Glasgow
 

[Ricardo Henriques-2]

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Hi Julia,

mEos2 is a photo-convertible genetically encoded fluorophore that switches from green to orange upon UV-light photo-activation, it is extremely popular in the super-resolution field (see the PALM) due to its high contrast and decent photon output. Alternatively, if you can live with fixed cells+ABs and a more stochastic/less controllable photo-switchable fluorophore, then classic Cy5 or Alexa647 will also do an excellent job (see the dSTORM techniques).

Here's a recent review with of some of the current photo-switchable fluorophores:
http://mhlangalab.synbio.csir.co.za/assets/publications/1/original/HenriquesMhlanga2009Reprinted.pdf?1266936429

Cheers,
R

Ricardo Henriques
Instituto de Medicina Molecular (Lisbon, Portugal).
For contact information see: https://sites.google.com/site/paxcalpt/

On Oct 4, 2011, at 12:29 PM, Julia Edgar wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All
> Can anybody recommend a good photo-switchable and/or photo-activatable reporter.
> Thank you
> Julia Edgar
> University of Glasgow
>

[Michelle Peckham]

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mEos2 works well for us, it fades quite quickly in the green, but is
nicely stable in the red after switching

On 04/10/2011 11:29, "Julia Edgar" <[hidden email]> wrote:

>*****
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>
>Dear All
>Can anybody recommend a good photo-switchable and/or photo-activatable
>reporter.
>Thank you
>Julia Edgar
>University of Glasgow
>

[David Solecki]

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I'd consider the mClavGR derivative from Robert Campbell's lab.......check out the Allele Biotech website for this FP.

*************************
David J. Solecki, Ph.D.
Assistant Member
Department of Developmental Neurobiology D2025C
St. Jude Children's Research Hospital
262 Danny Thomas Place-MS 323
Memphis, TN 38105
Office:901-595-5981
Lab: 901-595-4803
Fax: [phone]
website: www.stjude.org/solecki
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Michelle Peckham [[hidden email]]
Sent: Tuesday, October 04, 2011 6:08 AM
To: [hidden email]
Subject: Re: photo-switchable reporter.    .

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mEos2 works well for us, it fades quite quickly in the green, but is
nicely stable in the red after switching

On 04/10/2011 11:29, "Julia Edgar" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
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>*****
>
>Dear All
>Can anybody recommend a good photo-switchable and/or photo-activatable
>reporter.
>Thank you
>Julia Edgar
>University of Glasgow
>

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