microscope inverter

[RODRIGO VENA]

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Dear Listers!

any experience with this accessory/adapter? or similar??

http://www.lsmtech.com/product_objective_inverter.html

thank in advance!


Rodrigo Vena.-

[Barbara Foster]

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Yes, I have known of it for some time (although never "used it in
anger", as my old mentor would say).

I also have known Brad Bobbitt, who developed this technology, since
my early days at Zeiss (mid 1980's!).  He is known for his excellent
quality of work and customer service and depth of knowledge and
experience, specifically in confocal microscopy.

Hope this is helpful!
Barbara Foster, President & Chief Consultant
Microscopy/Microscopy Education  ... "Education, not Training"
7101 Royal Glen Trail, Suite A  - McKinney, TX 75070 - P: 972-924-5310
www.MicroscopyEducation.com


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At 07:55 AM 6/26/2015, you wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Dear Listers!
>
>any experience with this accessory/adapter? or similar??
>
>http://www.lsmtech.com/product_objective_inverter.html
>
>thank in advance!
>
>
>Rodrigo Vena.-

[Julio Vazquez-2]

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Hi Rodrigo,

We occasionally use the inverter on an Zeiss LSM 780 confocal system (to convert our system from inverted to upright). Haven't done much quantitative testing on it, but I can say that it worked fine for us. Should be fine on any infinity widefield or confocal system. If there is some loss of signal, it's certainly not very important. I would guess the loss of signal might be a bit more pronounced on a multi-photon system, but I haven't truly tested that. It comes with a side stage that can be mounted on the side of the normal stage to hold the specimen, and a platform to hold the microscope condenser (that must be removed to install the inverter). You need to make sure you have enough space around your scope to mount those accessories, and it helps to have a table with screw holes so that everything can be bolted. Obviously, if you have an X.Y motorized stage, you lose that functionality, but you can still do z-stacks. We found it relatively easy to install and use. Only difficulty was looking at the specimen in transmitted light. We eventually used a small portable LED lamp of the type used to help with jet lag placed under the specimen stage to get an acceptable brightfield image. I guess any bright, diffuse source would be OK.


Julio Vazquez
Scientific Imaging
Fred Hutchinson Cancer Research Center
Seattle, WA 98109

http://sharedresources.fhcrc.org/core-facilities/scientific-imaging




On Jun 26, 2015, at 7:41 AM, RODRIGO VENA wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Listers!
>
> any experience with this accessory/adapter? or similar??
>
> http://www.lsmtech.com/product_objective_inverter.html
>
> thank in advance!
>
>
> Rodrigo Vena.-