modulation (kHz) of a regular light-source for optogenetic-like experiments on a 2P Scope.

[lgelman]

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Hi everybody,

A colleague of mine, imaging with a 2-photon microscope calcium signaling in retina upon light stimulation has the following technical problem:

Retina needs to be stimulated with different patterns and colors between two scans. So the pattern needs to be modulated in the 2kHz range, when the scanning mirrors jump into a new row.
The current setup for light pattern stimulation consists of a normal beamer (used normally for PowerPoint presentations) where the light source has been replaced by a LED (Thorlabs) which can be easily modulated.
My colleague would like to be able to apply many more different wavelengths than the 3 available on the Thorlabs device. We will test other LED light sources from the market, but ideally, we would make use of a Polychrome V that is available here and would provide light from 320 to 680nm. Of course, the problem here is the modulation of the light source. We thought of a white laser, but we wish we could stick to cheap solutions and also to setups which could be easily mounted/transferred from one scope to the other one...

Many thanks in advance for your help!

Best regards,

Laurent.

_____________________________________________
Laurent Gelman, PhD
Head of Facility for Advanced Imaging and Microscopy
(Light Microscopy)

Friedrich Miescher Institut
WRO 1066.2.16
Maulbeerstrasse 66
CH-4058 Basel
Fix: [phone]
Mobile: [phone]
Fax: [phone]

www.fmi.ch<http://www.fmi.ch/>
www.microscopynetwork.unibas.ch/<http://www.microscopynetwork.unibas.ch/>

[Mike Buchin]

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No commercial interest on my part.  Prizmatix makes some high power multicolor LED modules with 30 KHz 5 microsecond switching times.

Mike

________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Gelman, Laurent [[hidden email]]
Sent: Monday, October 21, 2013 6:01 AM
To: [hidden email]
Subject: modulation (kHz) of a regular light-source for optogenetic-like experiments on a 2P Scope.

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Hi everybody,

A colleague of mine, imaging with a 2-photon microscope calcium signaling in retina upon light stimulation has the following technical problem:

Retina needs to be stimulated with different patterns and colors between two scans. So the pattern needs to be modulated in the 2kHz range, when the scanning mirrors jump into a new row.
The current setup for light pattern stimulation consists of a normal beamer (used normally for PowerPoint presentations) where the light source has been replaced by a LED (Thorlabs) which can be easily modulated.
My colleague would like to be able to apply many more different wavelengths than the 3 available on the Thorlabs device. We will test other LED light sources from the market, but ideally, we would make use of a Polychrome V that is available here and would provide light from 320 to 680nm. Of course, the problem here is the modulation of the light source. We thought of a white laser, but we wish we could stick to cheap solutions and also to setups which could be easily mounted/transferred from one scope to the other one...

Many thanks in advance for your help!

Best regards,

Laurent.

_____________________________________________
Laurent Gelman, PhD
Head of Facility for Advanced Imaging and Microscopy
(Light Microscopy)

Friedrich Miescher Institut
WRO 1066.2.16
Maulbeerstrasse 66
CH-4058 Basel
Fix: [phone]
Mobile: [phone]
Fax: [phone]

www.fmi.ch<http://www.fmi.ch/>
www.microscopynetwork.unibas.ch/<http://www.microscopynetwork.unibas.ch/>

[Johannes Helm]

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Grüezi, Laurent,

depending on how many kHz you need, a chopper wheel might also be
an alternative - and not so expensive - solution producing a virtually
perfect rectangular modulation intensity curve.

While I do not have any commercial affiliations, an example can be
found on:

http://www.newport.com/phase-locked-optical-
choppers/917676/1033/info.aspx?icid=srch-ban-0080


There do, as far as I recall, exist units which can chop up to 10kHz at
least.

I did use one of those - together with a lock in amplifier - when I did my
master thesis. However, this was in the mid 80s (laser saturation
spectroscopy, measuring some hyper fine structure transitions by means
of a Bennett Hole, laser was a CR18 pumped 699-21 single mode ring
dye laser by Coherent) and, while the combination of chopper wheel and
LIA worked nicely, I do not recall by heart now, which brand and type of
LIA and chopper wheel I had used. But it worked nicely, this I clearly
remember.

Best wishes,
Johannes

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi everybody,
>
> A colleague of mine, imaging with a 2-photon microscope calcium
> signaling in retina upon light stimulation has the following technical
> problem:
>
> Retina needs to be stimulated with different patterns and colors
> between two scans. So the pattern needs to be modulated in the
> 2kHz range, when the scanning mirrors jump into a new row.
> The current setup for light pattern stimulation consists of a normal
> beamer (used normally for PowerPoint presentations) where the light
> source has been replaced by a LED (Thorlabs) which can be easily
> modulated. My colleague would like to be able to apply many more
> different
> wavelengths than the 3 available on the Thorlabs device. We will test
> other LED light sources from the market, but ideally, we would make
> use of a Polychrome V that is available here and would provide light
> from 320 to 680nm. Of course, the problem here is the modulation of
> the light source. We thought of a white laser, but we wish we could
> stick to cheap solutions and also to setups which could be easily
> mounted/transferred from one scope to the other one...
>
> Many thanks in advance for your help!
>
> Best regards,
>
> Laurent.
>
> _____________________________________________
> Laurent Gelman, PhD
> Head of Facility for Advanced Imaging and Microscopy
> (Light Microscopy)
>
> Friedrich Miescher Institut
> WRO 1066.2.16
> Maulbeerstrasse 66
> CH-4058 Basel
> Fix: [phone]
> Mobile: [phone]
> Fax: [phone]
>
> www.fmi.ch<http://www.fmi.ch/>
>

www.microscopynetwork.unibas.ch/<http://www.microscopynetwork.unib
as.ch/>
>



--
P. Johannes Helm, M.Sc. PhD
Seniorengineer
CMBN
University of Oslo
Institute of Basic Medical Science
Department of Physiology
Postboks 1103 - Blindern
NO-0317 Oslo

Voice:        [phone]
Fax:        [phone]

WWW:        folk.uio.no/jhelm

[Barbara Foster]

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Dear Laurent,

I'm just finishing up an article on Sutter's DG4+, as applied to both
research bio and optogenetics.  I saw it at Neuro last year and was
very much impressed.  You might take a look at it.

No commercial involvement... editorial only.

Good hunting!
Barbara Foster, President & Chief Consultant
Microscopy/Microscopy Education*
www.MicroscopyEducation.com

*A subsidiary of The Microscopy & Imaging Place, Inc.
7101 Royal Glen Trail, Suite A
McKinney, TX 75070
P: 972-924-5310
F: 214-592-0277

MME is currently scheduling courses for now and through Spring 2014.
Call us today for a free training evaluation.

At 04:48 AM 10/21/2013, Gelman, Laurent wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Hi everybody,
>
>A colleague of mine, imaging with a 2-photon microscope calcium
>signaling in retina upon light stimulation has the following technical problem:
>
>Retina needs to be stimulated with different patterns and colors
>between two scans. So the pattern needs to be modulated in the 2kHz
>range, when the scanning mirrors jump into a new row.
>The current setup for light pattern stimulation consists of a normal
>beamer (used normally for PowerPoint presentations) where the light
>source has been replaced by a LED (Thorlabs) which can be easily modulated.
>My colleague would like to be able to apply many more different
>wavelengths than the 3 available on the Thorlabs device. We will
>test other LED light sources from the market, but ideally, we would
>make use of a Polychrome V that is available here and would provide
>light from 320 to 680nm. Of course, the problem here is the
>modulation of the light source. We thought of a white laser, but we
>wish we could stick to cheap solutions and also to setups which
>could be easily mounted/transferred from one scope to the other one...
>
>Many thanks in advance for your help!
>
>Best regards,
>
>Laurent.
>
>_____________________________________________
>Laurent Gelman, PhD
>Head of Facility for Advanced Imaging and Microscopy
>(Light Microscopy)
>
>Friedrich Miescher Institut
>WRO 1066.2.16
>Maulbeerstrasse 66
>CH-4058 Basel
>Fix: [phone]
>Mobile: [phone]
>Fax: [phone]
>
>www.fmi.ch<http://www.fmi.ch/>
>www.microscopynetwork.unibas.ch/<http://www.microscopynetwork.unibas.ch/>