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multi-slide holders

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Julio Vazquez

multi-slide holders

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal -

Dear listers,

I am looking for a commercially available slide holder that would have the footprint of a multiwell plate, accommodate let's say four standard 1"x3"slides, and be suitable for high-resolution imaging (e.g. with an oil immersion lens) on an inverted scope. The idea is to fit up to four slides on a plate stage adapter for semi-automated imaging on our Zeiss LSM. Thanks for any tips.

Julio.

Julio Vazquez,

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

http://www.fhcrc.org

Armstrong, Brian

Re: multi-slide holders

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Julio, we have an insert that looks just like this on an iCys Compucyte Cytometer; www.compucyte.com/icys.htm.

It is “Olympus sized” so I am not sure about adapting to a Zeiss. Cheers,

Brian D Armstrong PhD

Light Microscopy Core Manager

Beckman Research Institute

City of Hope

1450 E Duarte Rd

Duarte, CA 91010

626-359-8111 x62872

http://www.cityofhope.org/SharedResources/LightMicroscopy

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Julio Vazquez
Sent: Wednesday, July 30, 2008 2:58 PM
To: [hidden email]
Subject: multi-slide holders

Dear listers,

Julio.

Julio Vazquez,

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

http://www.fhcrc.org

---------------------------------------------------------------------
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DamirSudar

Re: multi-slide holders

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Julio,

You could check out ASI: http://www.asiimaging.com/slideinserts.html and
look at the SI-INV-4000. It fits into a standard Zeiss scanning stage on
an inverted. While it works, I found it a little clumsy to get slides in
and out so we ended up having our own design made. Let me know if you
like more info on that home-made design.

Cheers,
- Damir Sudar
Lawrence Berkeley Natl Lab

Armstrong, Brian wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Julio, we have an insert that looks just like this on an iCys
> Compucyte Cytometer; www.compucyte.com/*icys*.htm
> <http://www.compucyte.com/icys.htm>.
>
> It is “Olympus sized” so I am not sure about adapting to a Zeiss. Cheers,
>
>
>
> Brian D Armstrong PhD
>
> Light Microscopy Core Manager
>
> Beckman Research Institute
>
> City of Hope
>
> 1450 E Duarte Rd
>
> Duarte, CA 91010
>
> 626-359-8111 x62872
>
> http://www.cityofhope.org/SharedResources/LightMicroscopy
>
>
>
> ------------------------------------------------------------------------
>
> *From:* Confocal Microscopy List [mailto:[hidden email]]
> *On Behalf Of *Julio Vazquez
> *Sent:* Wednesday, July 30, 2008 2:58 PM
> *To:* [hidden email]
> *Subject:* multi-slide holders
>
>
>
> -
>
> Dear listers,
>
>
>
> I am looking for a commercially available slide holder that would have
> the footprint of a multiwell plate, accommodate let's say four standard
> 1"x3"slides, and be suitable for high-resolution imaging (e.g. with an
> oil immersion lens) on an inverted scope. The idea is to fit up to four
> slides on a plate stage adapter for semi-automated imaging on our Zeiss
> LSM. Thanks for any tips.
>
>
> Julio.
>
> --
>
> Julio Vazquez,
>
> Fred Hutchinson Cancer Research Center
>
> Seattle, WA 98109-1024
>
>
>
> http://www.fhcrc.org
>
>
>
>
>
>
> ---------------------------------------------------------------------
> SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are
> intended solely for the individual or entity to which they are
> addressed. This communication may contain information that is
> privileged, confidential, or exempt from disclosure under applicable law
> (e.g., personal health information, research data, financial
> information). Because this e-mail has been sent without encryption,
> individuals other than the intended recipient may be able to view the
> information, forward it to others or tamper with the information without
> the knowledge or consent of the sender. If you are not the intended
> recipient, or the employee or person responsible for delivering the
> message to the intended recipient, any dissemination, distribution or
> copying of the communication is strictly prohibited. If you received the
> communication in error, please notify the sender immediately by replying
> to this message and deleting the message and any accompanying files from
> your system. If, due to the security risks, you do not wish to receive
> further communications via e-mail, please reply to this message and
> inform the sender that you do not wish to receive further e-mail from
> the sender.
>
> ---------------------------------------------------------------------
>

--
Damir Sudar - Staff Scientist
Lawrence Berkeley Laboratory / Life Sciences Division
One Cyclotron Road, MS 977R225A, Berkeley, CA 94720, USA
T: 510/486-5346 - F: 510/486-5586 - E: [hidden email]
WWW: http://www.lbl.gov/lifesciences/labs/sudar_lab.html

Wolf Heusermann

Re: multi-slide holders

In reply to this post by Julio Vazquez

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Julio,

We've desined a multi slide holder stage insert for a 160x110 recently.
We could easily adapt this one for your needs and produce it. If you
have questions or want to see pictures please contact me at
[hidden email]

Wolf

Julio Vazquez schrieb:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal -
> Dear listers,
>
> I am looking for a commercially available slide holder that would have
> the footprint of a multiwell plate, accommodate let's say four
> standard 1"x3"slides, and be suitable for high-resolution imaging
> (e.g. with an oil immersion lens) on an inverted scope. The idea is to
> fit up to four slides on a plate stage adapter for semi-automated
> imaging on our Zeiss LSM. Thanks for any tips.
>
> Julio.
> --
> Julio Vazquez,
> Fred Hutchinson Cancer Research Center
> Seattle, WA 98109-1024
>
> http://www.fhcrc.org
>
>
> ------------------------------------------------------------------------
>
> No virus found in this incoming message.
> Checked by AVG.
> Version: 7.5.524 / Virus Database: 270.5.6/1578 - Release Date: 28.07.2008 17:13
>

Wolf Heusermann

Re: multi-slide holders

In reply to this post by Julio Vazquez

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Julio,

We've designed a multi slide holder stage insert for a 160x110 recently.
We could easily adapt this one for your needs and produce it. If you
have questions or want to see pictures please contact me at
[hidden email]

Wolf

Julio Vazquez

Re: multi-slide holders

In reply to this post by Armstrong, Brian

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Brian,

Thanks for the tip. I actually got contacted by someone from Compucyte regarding the slide holder.

As a matter of fact, I am also shopping for an HCS instrument. Since you have an iCys, could I ask (without taking too much of your time) for your opinion on the instrument, such as its main uses (live, fixed, plates, slides), and its main strengths and weaknesses (ease of use, image quality, software...). That might help narrow down my options.

By the way, your web link does not seem to be valid any longer. Here is how I got to the City Of Hope Imaging web site:

http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx

and

http://www.cityofhope.org/research/support/AC/Pages/default.aspx

Best wishes,

julio.

Julio Vazquez, PhD

Director of Scientific Imaging

Fred Hutchinson Cancer Research Center

1100 Fairview Ave. N., mailstop DE-512

Seattle, WA 98109-1024

Tel: Office: 206-667-1215/ Lab: 206-667-4205

FAX: 206-667-6845

[hidden email]

http://www.fhcrc.org/science/shared_resources/imaging/

--------------------------------------------------
DISCLAIMER:

This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this information is strictly prohibited. If you received this message in error, please notify the sender then delete this message.

On Jul 30, 2008, at 3:56 PM, Armstrong, Brian wrote:

Hi Julio, we have an insert that looks just like this on an iCys Compucyte Cytometer; www.compucyte.com/icys.htm.
It is “Olympus sized” so I am not sure about adapting to a Zeiss. Cheers,

Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of Hope
1450 E Duarte Rd
Duarte, CA 91010
626-359-8111 x62872
http://www.cityofhope.org/SharedResources/LightMicroscopy

Armstrong, Brian

Re: multi-slide holders

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Julio, yes we changed our site. Thanks for pointing it out I had forgotten about my sig link.

The iCys is not used that much here at COH. I think that people were initially optimistic, but have failed to use it much.

-Pretty easy to use, gives data much like FACS (I think this machine is more for FACS types). Out system is controlled by our Flow Core.

- Image quality is ok. Olympus based (think IX71). Ours is not confocal.

-Software is decent and user friendly once you figure it out (is that always the case?).

We just purchased the Molecular Dynamics HCS instrument after much demonstration. MD has; good price, good software analysis, robotic adaptation, and our sales/tech person was very knowledgeable.

Nick Callamaras PhD 800-635-5577 x3566

[hidden email]

For more on the HCS instrument I can refer you to our manager of the HCS core.

For more on the iCys I can refer you to the tech that operates it daily.

Cheers,

Brian D Armstrong PhD

Light Microscopy Core Manager

Beckman Research Institute

City of Hope

1450 E Duarte Rd

Duarte, CA 91010

626-359-8111 x62872

http://www.cityofhope.org/SharedResources/LightMicroscopy

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Julio Vazquez
Sent: Thursday, July 31, 2008 1:19 PM
To: [hidden email]
Subject: Re: multi-slide holders

Hi Brian,

Thanks for the tip. I actually got contacted by someone from Compucyte regarding the slide holder.

By the way, your web link does not seem to be valid any longer. Here is how I got to the City Of Hope Imaging web site:

http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx

and

http://www.cityofhope.org/research/support/AC/Pages/default.aspx

Best wishes,

julio.

Julio Vazquez, PhD

Director of Scientific Imaging

Fred Hutchinson Cancer Research Center

1100 Fairview Ave. N., mailstop DE-512

Seattle, WA 98109-1024

Tel: Office: 206-667-1215/ Lab: 206-667-4205

FAX: 206-667-6845

[hidden email]

http://www.fhcrc.org/science/shared_resources/imaging/

--------------------------------------------------
DISCLAIMER:

On Jul 30, 2008, at 3:56 PM, Armstrong, Brian wrote:

Hi Julio, we have an insert that looks just like this on an iCys Compucyte Cytometer; www.compucyte.com/icys.htm.

It is “Olympus sized” so I am not sure about adapting to a Zeiss. Cheers,

Brian D Armstrong PhD

Light Microscopy Core Manager

Beckman Research Institute

City of Hope

1450 E Duarte Rd

Duarte, CA 91010

626-359-8111 x62872

http://www.cityofhope.org/SharedResources/LightMicroscopy

---------------------------------------------------------------------

Julio Vazquez

Re: multi-slide holders

In reply to this post by Julio Vazquez

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

My apologies... that message was meant for Brian.

Many thanks to all those who provided useful tips.

Julio.

Julio Vazquez,

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

http://www.fhcrc.org

On Jul 31, 2008, at 1:19 PM, Julio Vazquez wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Brian,

Thanks for the tip. I actually got contacted by someone from Compucyte regarding the slide holder.

As a matter of fact, I am also shopping for an HCS instrument. Since you have an iCys, could I ask (without taking too much of your time) for your opinion on the instrument, such as its main uses (live, fixed, plates, slides), and its main strengths and weaknesses (ease of use, image quality, software...). That might help narrow down my options.

By the way, your web link does not seem to be valid any longer. Here is how I got to the City Of Hope Imaging web site:

http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx

and

http://www.cityofhope.org/research/support/AC/Pages/default.aspx

Best wishes,

julio.

--
Julio Vazquez, PhD
Director of Scientific Imaging
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N., mailstop DE-512
Seattle, WA 98109-1024

Tel: Office: 206-667-1215/ Lab: 206-667-4205
FAX: 206-667-6845

[hidden email]
http://www.fhcrc.org/science/shared_resources/imaging/

--------------------------------------------------
DISCLAIMER:
This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this information is strictly prohibited. If you received this message in error, please notify the sender then delete this message.

On Jul 30, 2008, at 3:56 PM, Armstrong, Brian wrote:

Hi Julio, we have an insert that looks just like this on an iCys Compucyte Cytometer; www.compucyte.com/icys.htm.
It is “Olympus sized” so I am not sure about adapting to a Zeiss. Cheers,

Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of Hope
1450 E Duarte Rd
Duarte, CA 91010
626-359-8111 x62872
http://www.cityofhope.org/SharedResources/LightMicroscopy

Todorov, Ivan

Re: iCys

In reply to this post by Armstrong, Brian

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Julio,

I am one of the people keeping the iCys busy quite a bit (no commercial interest) for already more than two years.

The machine is reliable (did not have problems after more than 4000 runs) and could get lots of quantitation (of the type you get from a FACS) out of slides with cells or tissue sections, as well as from dishes and plates. It is based on an IX71 with lenses ranging from 4x to 60x and three lasers (405, 488 and 633). Image quality is not bad, but when I need a picture for a paper, use our old upright BX50. The iCys is a cytometer anyhow ...

The software is working fine (once you get familiar with!). The Compucyte guys are very helpful and start with a week of training at their place.

Will be happy to chat about anytime

All the best

Yours

Ivan

Ivan Todorov, Ph.D.
Department of Diabetes, Endocrinology and Metabolism
City of Hope
1500 Duarte Rd
Duarte, CA 91010
tel: 626-359-8111 ext 65538
fax: 626-301-8136
email: [hidden email]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]On Behalf Of Armstrong, Brian
Sent: Thursday, July 31, 2008 1:28 PM
To: [hidden email]
Subject: Re: multi-slide holders

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Julio, yes we changed our site. Thanks for pointing it out I had forgotten about my sig link.

The iCys is not used that much here at COH. I think that people were initially optimistic, but have failed to use it much.

-Pretty easy to use, gives data much like FACS (I think this machine is more for FACS types). Out system is controlled by our Flow Core.

- Image quality is ok. Olympus based (think IX71). Ours is not confocal.

-Software is decent and user friendly once you figure it out (is that always the case?).

We just purchased the Molecular Dynamics HCS instrument after much demonstration. MD has; good price, good software analysis, robotic adaptation, and our sales/tech person was very knowledgeable.

Nick Callamaras PhD 800-635-5577 x3566

[hidden email]

For more on the HCS instrument I can refer you to our manager of the HCS core.

For more on the iCys I can refer you to the tech that operates it daily.

Cheers,

Brian D Armstrong PhD

Light Microscopy Core Manager

Beckman Research Institute

City of Hope

1450 E Duarte Rd

Duarte, CA 91010

626-359-8111 x62872

http://www.cityofhope.org/SharedResources/LightMicroscopy

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Julio Vazquez
Sent: Thursday, July 31, 2008 1:19 PM
To: [hidden email]
Subject: Re: multi-slide holders

Hi Brian,

Thanks for the tip. I actually got contacted by someone from Compucyte regarding the slide holder.

As a matter of fact, I am also shopping for an HCS instrument. Since you have an iCys, could I ask (without taking too much of your time) for your opinion on the instrument, such as its main uses (live, fixed, plates, slides), and its main strengths and weaknesses (ease of use, image quality, software...). That might help narrow down my options.

By the way, your web link does not seem to be valid any longer. Here is how I got to the City Of Hope Imaging web site:

http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx

and

http://www.cityofhope.org/research/support/AC/Pages/default.aspx

Best wishes,

julio.

--

Julio Vazquez, PhD

Director of Scientific Imaging

Fred Hutchinson Cancer Research Center

1100 Fairview Ave. N., mailstop DE-512

Seattle, WA 98109-1024

Tel: Office: 206-667-1215/ Lab: 206-667-4205

FAX: 206-667-6845

[hidden email]

http://www.fhcrc.org/science/shared_resources/imaging/

--------------------------------------------------
DISCLAIMER:

This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this information is strictly prohibited. If you received this message in error, please notify the sender then delete this message.

On Jul 30, 2008, at 3:56 PM, Armstrong, Brian wrote:

Hi Julio, we have an insert that looks just like this on an iCys Compucyte Cytometer; www.compucyte.com/icys.htm.

It is “Olympus sized” so I am not sure about adapting to a Zeiss. Cheers,

Brian D Armstrong PhD

Light Microscopy Core Manager

Beckman Research Institute

City of Hope

1450 E Duarte Rd

Duarte, CA 91010

626-359-8111 x62872

http://www.cityofhope.org/SharedResources/LightMicroscopy

---------------------------------------------------------------------
SECURITY/CONFIDENTIALITY WARNING: This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender.
---------------------------------------------------------------------

Raffi Manoukian

Re: iCys

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear listers,
I’ve been following the discussions regarding the quality of imaging on
CompuCyte’s laser scanning cytometry platform and I’d just like to share my
thoughts with you. Until recently, I was working in the core imaging and flow
facility at Amgen Inc. in Southern California. We had both confocal systems
and several LSC instruments (iCyte, iCys). I’ve been working with the LSC for
over 8 years now and I believe the image quality ultimately comes down to
the sample prep. If the sample prep is clean, and the staining is good, there is
no doubt that you will obtain an image of very high quality (on both
platforms). Using a 40x objective, with the iCys’s variable stage resolution set
at 0.5uM (it has a motorized microstep stage), you can obtain incredibly
sharp, clear images of your samples, whether it be cells or tissue (some people
have even thought that some of my LSC images were from a confocal
system!). Clearly, the confocal will give you extra-sharp and clean images
because you’re looking at a very thin slice through your sample; with
CompuCyte’s iGeneration instruments, you’re sampling a thicker focal slice of
your sample, because that is what the instrument is designed to do: to collect
signal through a wide depth of field in order to accurately quantitate the
signal. That is why you can do stoichiometric measurements on adherent cells
like cell cycle analysis on the iCys or iCyte. The great thing about the
technology is that you can then correlate your cytometric data to its
corresponding image, instantaneously, without third party software. So in a
nutshell…. Great high resolution images AND quantitative data in the same
scan. I am now actually an applications scientist with CompuCyte and I’d be
happy to answer any further questions, but you don’t have to take my word
for it, visit www.compucyte.com and www.imagingcytometrycenter.com
Regards,

Raffi Manoukian

Raffi Manoukian, M.Sc.
Senior Applications Scientist
CompuCyte Corporation
949-573-1895