nail polish

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Valeria Berno Valeria Berno
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nail polish

Hi,

sorry for the practical and banal question....

Where do you buy the nail polish??at the grocery? if yes, is there in
Europe a brand that you can suggest me....
is there any supplier on the web?


Thanks

Valeria
Guenter Giese Guenter Giese
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AW: nail polish

Hi Valeria,

I recommend to buy different brands of nail hardener (non-fluorescing) at a
beauty shop or grocery store and do a brief test with each brand:

fix a cover slip on a slide with the nail hardener (like you prepare your
sample). Let dry overnight, and put under water next day. The one prep
dissolving latest (or not at all) will be the best one.

This test really pays off in my experience.

Best,

Guenter



------------------------------------------
Dr. Guenter Giese
Light Microscopy Facility Manager
Dept. of Biomedical Optics
MPI fuer Medizinische Forschung Jahnstr. 29
D-69120 Heidelberg, Germany
Phone (+49) 6221-486-360 (Fax: -325)
e-mail: [hidden email]  

> -----Ursprüngliche Nachricht-----
> Von: Confocal Microscopy List
> [mailto:[hidden email]] Im Auftrag von Valeria Berno
> Gesendet: Montag, 16. März 2009 10:15
> An: [hidden email]
> Betreff: nail polish
>
> Hi,
>
> sorry for the practical and banal question....
>
> Where do you buy the nail polish??at the grocery? if yes, is
> there in Europe a brand that you can suggest me....
> is there any supplier on the web?
>
>
> Thanks
>
> Valeria
>
Carol Heckman Carol Heckman
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Re: nail polish

In reply to this post by Valeria Berno
Valeria-
If you want it for mounting samples for fluorescence microscopy, it would be wise to buy good quality nail polish at the beauty parlor.  The cheap stuff sold in the grocery store has a solvent that will destroy your fluorochrome, or maybe the whole cell depending on the concentration that diffuses underneath your coverslips.
Carol Heckman
Bowling Green State University
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Valeria Berno [[hidden email]]
Sent: Monday, March 16, 2009 5:14 AM
To: [hidden email]
Subject: nail polish

Hi,

sorry for the practical and banal question....

Where do you buy the nail polish??at the grocery? if yes, is there in
Europe a brand that you can suggest me....
is there any supplier on the web?


Thanks

Valeria
Gens, John Scott Gens, John Scott
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Re: nail polish

In reply to this post by Valeria Berno
Valencia-

The last time I picked some up nail polish I had such a hard time
finding one that did not have some form of plasticizer or other
additive in it that gave me serious autofluorescence problems.  I ended
up going to the hardware and buying a small container of clear lacquer
(usually in the paint/wood stain aisle) instead, and decanting it into
many small nail-polish type bottles. Much cheaper per ounce and weirdly
enough in the US at least all ingredients are clearly identified in the
hardware product but not on the beauty product.

It seems to work fine for fixed samples, caveat is that the solvent in
either product is bad news for live-cell work. For live cells, I'd
recommend using "valap", a 1:1:1 homebrew mixture of vaseline, lanolin,
and parrafin.

Scott

--
J. Scott Gens, Ph.D.
Biocomplexity Institute
Indiana University Physics
Office:     Room 047H, Simon Interdisciplinary Sciences Bldg.
Multi-photon Confocal: 063 Swain Hall West.
Bloomington IN 47405
812-855-7946
[hidden email]



Quoting Valeria Berno <[hidden email]>:

> Hi,
>
> sorry for the practical and banal question....
>
> Where do you buy the nail polish??at the grocery? if yes, is there in
> Europe a brand that you can suggest me....
> is there any supplier on the web?
>
>
> Thanks
>
> Valeria
>
Todorov, Ivan Todorov, Ivan
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Re: nail polish

In reply to this post by Valeria Berno
Hi Valeria,
We usually get a brand name clear nail polish from the pharmacy/grocery (Whatever is on sale!). The current one we use is Revlon and works great (no commercial interest). Do not remember what I used in Europe, but kept the same rule. Just stay away from the cheap ones.
Best
Ivan

Ivan Todorov, Ph.D.
Department of Diabetes, Endocrinology and Metabolism
City of Hope
1500 Duarte Rd
Duarte, CA 91010
tel: 626-359-8111 ext 65538
fax: 626-301-8136
email: [hidden email]



-----Original Message-----
From: Confocal Microscopy List
[mailto:[hidden email]]On Behalf Of Valeria Berno
Sent: Monday, March 16, 2009 2:15 AM
To: [hidden email]
Subject: nail polish


Hi,

sorry for the practical and banal question....

Where do you buy the nail polish??at the grocery? if yes, is there in
Europe a brand that you can suggest me....
is there any supplier on the web?


Thanks

Valeria


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Rosemary.White Rosemary.White
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Re: nail polish

In reply to this post by Gens, John Scott
Scott - a great idea re. nail polish!  I usually go down to the $2 shop and
get 10 of whatever brand's on special (as long as it's colourless).
cheers,
Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334



On 18/03/09 4:44 AM, "J. Scott Gens, PhD" <[hidden email]> wrote:

> Valencia-
>
> The last time I picked some up nail polish I had such a hard time
> finding one that did not have some form of plasticizer or other
> additive in it that gave me serious autofluorescence problems.  I ended
> up going to the hardware and buying a small container of clear lacquer
> (usually in the paint/wood stain aisle) instead, and decanting it into
> many small nail-polish type bottles. Much cheaper per ounce and weirdly
> enough in the US at least all ingredients are clearly identified in the
> hardware product but not on the beauty product.
>
> It seems to work fine for fixed samples, caveat is that the solvent in
> either product is bad news for live-cell work. For live cells, I'd
> recommend using "valap", a 1:1:1 homebrew mixture of vaseline, lanolin,
> and parrafin.
>
> Scott
>
> --
> J. Scott Gens, Ph.D.
> Biocomplexity Institute
> Indiana University Physics
> Office:     Room 047H, Simon Interdisciplinary Sciences Bldg.
> Multi-photon Confocal: 063 Swain Hall West.
> Bloomington IN 47405
> 812-855-7946
> [hidden email]
>
>
>
> Quoting Valeria Berno <[hidden email]>:
>
>> Hi,
>>
>> sorry for the practical and banal question....
>>
>> Where do you buy the nail polish??at the grocery? if yes, is there in
>> Europe a brand that you can suggest me....
>> is there any supplier on the web?
>>
>>
>> Thanks
>>
>> Valeria
>>
Guy Cox Guy Cox
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Re: nail polish

Why use colourless?  I usually use something nice and bright, so that you can see at a glance that the slide is sealed.  It also gives you immediate warning when you are getting close to the edge of the coverslip.  Mind you I do sometimes get funny looks when people see blue and orange nail polish bottles on my desk!

                                               Guy



Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
     http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Rosemary White
Sent: Wednesday, 18 March 2009 8:45 AM
To: [hidden email]
Subject: Re: nail polish

Scott - a great idea re. nail polish!  I usually go down to the $2 shop and get 10 of whatever brand's on special (as long as it's colourless).
cheers,
Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334



On 18/03/09 4:44 AM, "J. Scott Gens, PhD" <[hidden email]> wrote:

> Valencia-
>
> The last time I picked some up nail polish I had such a hard time
> finding one that did not have some form of plasticizer or other
> additive in it that gave me serious autofluorescence problems.  I
> ended up going to the hardware and buying a small container of clear
> lacquer (usually in the paint/wood stain aisle) instead, and decanting
> it into many small nail-polish type bottles. Much cheaper per ounce
> and weirdly enough in the US at least all ingredients are clearly
> identified in the hardware product but not on the beauty product.
>
> It seems to work fine for fixed samples, caveat is that the solvent in
> either product is bad news for live-cell work. For live cells, I'd
> recommend using "valap", a 1:1:1 homebrew mixture of vaseline,
> lanolin, and parrafin.
>
> Scott
>
> --
> J. Scott Gens, Ph.D.
> Biocomplexity Institute
> Indiana University Physics
> Office:     Room 047H, Simon Interdisciplinary Sciences Bldg.
> Multi-photon Confocal: 063 Swain Hall West.
> Bloomington IN 47405
> 812-855-7946
> [hidden email]
>
>
>
> Quoting Valeria Berno <[hidden email]>:
>
>> Hi,
>>
>> sorry for the practical and banal question....
>>
>> Where do you buy the nail polish??at the grocery? if yes, is there in
>> Europe a brand that you can suggest me....
>> is there any supplier on the web?
>>
>>
>> Thanks
>>
>> Valeria
>>

No virus found in this incoming message.
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No virus found in this outgoing message.
Checked by AVG.
Version: 7.5.557 / Virus Database: 270.11.18/2008 - Release Date: 17/03/2009 4:25 PM
 
Michael Schell Michael Schell
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Re: nail polish

In reply to this post by Valeria Berno
I like quick drying dark colors.  My favorite brand is "Paint & Go":  paint your slides, then go to the microscope 5 min later.

Michael J. Schell, Ph.D., CIV, USUHS
Assist. Professor
Dept. of Pharmacology
Uniformed Services University
4301 Jones Bridge Rd.
Bethesda, MD  20814-3220
tel:  (301) 295-3249
[hidden email]
>>> Guy Cox <[hidden email]> 03/17/09 7:11 PM >>>
Why use colourless?  I usually use something nice and bright, so that you can see at a glance that the slide is sealed.  It also gives you immediate warning when you are getting close to the edge of the coverslip.  Mind you I do sometimes get funny looks when people see blue and orange nail polish bottles on my desk!

                                               Guy



Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176     Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
     http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Rosemary White
Sent: Wednesday, 18 March 2009 8:45 AM
To: [hidden email]
Subject: Re: nail polish

Scott - a great idea re. nail polish!  I usually go down to the $2 shop and get 10 of whatever brand's on special (as long as it's colourless).
cheers,
Rosemary

Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

ph 61 2 6246 5475
fx 61 2 6246 5334



On 18/03/09 4:44 AM, "J. Scott Gens, PhD" <[hidden email]> wrote:

> Valencia-
>
> The last time I picked some up nail polish I had such a hard time
> finding one that did not have some form of plasticizer or other
> additive in it that gave me serious autofluorescence problems.  I
> ended up going to the hardware and buying a small container of clear
> lacquer (usually in the paint/wood stain aisle) instead, and decanting
> it into many small nail-polish type bottles. Much cheaper per ounce
> and weirdly enough in the US at least all ingredients are clearly
> identified in the hardware product but not on the beauty product.
>
> It seems to work fine for fixed samples, caveat is that the solvent in
> either product is bad news for live-cell work. For live cells, I'd
> recommend using "valap", a 1:1:1 homebrew mixture of vaseline,
> lanolin, and parrafin.
>
> Scott
>
> --
> J. Scott Gens, Ph.D.
> Biocomplexity Institute
> Indiana University Physics
> Office:     Room 047H, Simon Interdisciplinary Sciences Bldg.
> Multi-photon Confocal: 063 Swain Hall West.
> Bloomington IN 47405
> 812-855-7946
> [hidden email]
>
>
>
> Quoting Valeria Berno <[hidden email]>:
>
>> Hi,
>>
>> sorry for the practical and banal question....
>>
>> Where do you buy the nail polish??at the grocery? if yes, is there in
>> Europe a brand that you can suggest me....
>> is there any supplier on the web?
>>
>>
>> Thanks
>>
>> Valeria
>>

No virus found in this incoming message.
Checked by AVG.
Version: 7.5.557 / Virus Database: 270.11.16/2005 - Release Date: 16/03/2009 7:01 PM
 

No virus found in this outgoing message.
Checked by AVG.
Version: 7.5.557 / Virus Database: 270.11.18/2008 - Release Date: 17/03/2009 4:25 PM
 
Paul Maddox Paul Maddox
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Re: nail polish

In reply to this post by Guy Cox
I could not agree more!  I like to us different colors, depending on  
my mood of course.

Sent from my iPod

On Mar 17, 2009, at 7:10 PM, "Guy Cox" <[hidden email]> wrote:

> Why use colourless?  I usually use something nice and bright, so  
> that you can see at a glance that the slide is sealed.  It also  
> gives you immediate warning when you are getting close to the edge  
> of the coverslip.  Mind you I do sometimes get funny looks when  
> people see blue and orange nail polish bottles on my desk!
>
>                                               Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor & Francis
>    http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176     Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
>     http://www.guycox.net
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]
> ] On Behalf Of Rosemary White
> Sent: Wednesday, 18 March 2009 8:45 AM
> To: [hidden email]
> Subject: Re: nail polish
>
> Scott - a great idea re. nail polish!  I usually go down to the $2  
> shop and get 10 of whatever brand's on special (as long as it's  
> colourless).
> cheers,
> Rosemary
>
> Rosemary White
> CSIRO Plant Industry
> GPO Box 1600
> Canberra, ACT 2601
> Australia
>
> ph 61 2 6246 5475
> fx 61 2 6246 5334
>
>
>
> On 18/03/09 4:44 AM, "J. Scott Gens, PhD" <[hidden email]> wrote:
>
>> Valencia-
>>
>> The last time I picked some up nail polish I had such a hard time
>> finding one that did not have some form of plasticizer or other
>> additive in it that gave me serious autofluorescence problems.  I
>> ended up going to the hardware and buying a small container of clear
>> lacquer (usually in the paint/wood stain aisle) instead, and  
>> decanting
>> it into many small nail-polish type bottles. Much cheaper per ounce
>> and weirdly enough in the US at least all ingredients are clearly
>> identified in the hardware product but not on the beauty product.
>>
>> It seems to work fine for fixed samples, caveat is that the solvent  
>> in
>> either product is bad news for live-cell work. For live cells, I'd
>> recommend using "valap", a 1:1:1 homebrew mixture of vaseline,
>> lanolin, and parrafin.
>>
>> Scott
>>
>> --
>> J. Scott Gens, Ph.D.
>> Biocomplexity Institute
>> Indiana University Physics
>> Office:     Room 047H, Simon Interdisciplinary Sciences Bldg.
>> Multi-photon Confocal: 063 Swain Hall West.
>> Bloomington IN 47405
>> 812-855-7946
>> [hidden email]
>>
>>
>>
>> Quoting Valeria Berno <[hidden email]>:
>>
>>> Hi,
>>>
>>> sorry for the practical and banal question....
>>>
>>> Where do you buy the nail polish??at the grocery? if yes, is there  
>>> in
>>> Europe a brand that you can suggest me....
>>> is there any supplier on the web?
>>>
>>>
>>> Thanks
>>>
>>> Valeria
>>>
>
> No virus found in this incoming message.
> Checked by AVG.
> Version: 7.5.557 / Virus Database: 270.11.16/2005 - Release Date:  
> 16/03/2009 7:01 PM
>
>
> No virus found in this outgoing message.
> Checked by AVG.
> Version: 7.5.557 / Virus Database: 270.11.18/2008 - Release Date:  
> 17/03/2009 4:25 PM
>
Rosemary.White Rosemary.White
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Re: nail polish

Well, I used to get the colourful ones, then had a batch that leaked colour
into the preparation, so have stuck to the boring clear stuff ever since....


On 18/03/09 10:27 AM, "Maddox Paul" <[hidden email]> wrote:

> I could not agree more!  I like to us different colors, depending on
> my mood of course.
>
> Sent from my iPod
>
> On Mar 17, 2009, at 7:10 PM, "Guy Cox" <[hidden email]> wrote:
>
>> Why use colourless?  I usually use something nice and bright, so
>> that you can see at a glance that the slide is sealed.  It also
>> gives you immediate warning when you are getting close to the edge
>> of the coverslip.  Mind you I do sometimes get funny looks when
>> people see blue and orange nail polish bottles on my desk!
>>
>>                                               Guy
>>
>>
>>
>> Optical Imaging Techniques in Cell Biology
>> by Guy Cox    CRC Press / Taylor & Francis
>>    http://www.guycox.com/optical.htm
>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>> Electron Microscope Unit, Madsen Building F09,
>> University of Sydney, NSW 2006
>> ______________________________________________
>> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>> Mobile 0413 281 861
>> ______________________________________________
>>     http://www.guycox.net
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[hidden email]
>> ] On Behalf Of Rosemary White
>> Sent: Wednesday, 18 March 2009 8:45 AM
>> To: [hidden email]
>> Subject: Re: nail polish
>>
>> Scott - a great idea re. nail polish!  I usually go down to the $2
>> shop and get 10 of whatever brand's on special (as long as it's
>> colourless).
>> cheers,
>> Rosemary
>>
>> Rosemary White
>> CSIRO Plant Industry
>> GPO Box 1600
>> Canberra, ACT 2601
>> Australia
>>
>> ph 61 2 6246 5475
>> fx 61 2 6246 5334
>>
>>
>>
>> On 18/03/09 4:44 AM, "J. Scott Gens, PhD" <[hidden email]> wrote:
>>
>>> Valencia-
>>>
>>> The last time I picked some up nail polish I had such a hard time
>>> finding one that did not have some form of plasticizer or other
>>> additive in it that gave me serious autofluorescence problems.  I
>>> ended up going to the hardware and buying a small container of clear
>>> lacquer (usually in the paint/wood stain aisle) instead, and
>>> decanting
>>> it into many small nail-polish type bottles. Much cheaper per ounce
>>> and weirdly enough in the US at least all ingredients are clearly
>>> identified in the hardware product but not on the beauty product.
>>>
>>> It seems to work fine for fixed samples, caveat is that the solvent
>>> in
>>> either product is bad news for live-cell work. For live cells, I'd
>>> recommend using "valap", a 1:1:1 homebrew mixture of vaseline,
>>> lanolin, and parrafin.
>>>
>>> Scott
>>>
>>> --
>>> J. Scott Gens, Ph.D.
>>> Biocomplexity Institute
>>> Indiana University Physics
>>> Office:     Room 047H, Simon Interdisciplinary Sciences Bldg.
>>> Multi-photon Confocal: 063 Swain Hall West.
>>> Bloomington IN 47405
>>> 812-855-7946
>>> [hidden email]
>>>
>>>
>>>
>>> Quoting Valeria Berno <[hidden email]>:
>>>
>>>> Hi,
>>>>
>>>> sorry for the practical and banal question....
>>>>
>>>> Where do you buy the nail polish??at the grocery? if yes, is there
>>>> in
>>>> Europe a brand that you can suggest me....
>>>> is there any supplier on the web?
>>>>
>>>>
>>>> Thanks
>>>>
>>>> Valeria
>>>>
>>
>> No virus found in this incoming message.
>> Checked by AVG.
>> Version: 7.5.557 / Virus Database: 270.11.16/2005 - Release Date:
>> 16/03/2009 7:01 PM
>>
>>
>> No virus found in this outgoing message.
>> Checked by AVG.
>> Version: 7.5.557 / Virus Database: 270.11.18/2008 - Release Date:
>> 17/03/2009 4:25 PM
>>
Moninger, Thomas Moninger, Thomas
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Re: nail polish

In reply to this post by Paul Maddox

OK, let's mention brands.
 
Wet N' Wild is our nail polish of choice. In our hands it has no effect on signal, including when used with fluorescent proteins. No comment on colors. 
 
However, it better be dry before it comes anywhere near my objectives lenses........
 
Cheers,
 
Tom

  _____  

From: Confocal Microscopy List on behalf of Maddox Paul
Sent: Tue 3/17/2009 6:27 PM
To: [hidden email]
Subject: Re: nail polish



I could not agree more!  I like to us different colors, depending on 
my mood of course.

Sent from my iPod

On Mar 17, 2009, at 7:10 PM, "Guy Cox" <[hidden email]> wrote:

> Why use colourless?  I usually use something nice and bright, so 
> that you can see at a glance that the slide is sealed.  It also 
> gives you immediate warning when you are getting close to the edge 
> of the coverslip.  Mind you I do sometimes get funny looks when 
> people see blue and orange nail polish bottles on my desk!
>
>                                               Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor & Francis
>   
http://www.guycox.com/optical.htm
>

Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged.  If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited.  Please reply to the sender that you have received the message in error, then delete it.  Thank you.

______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176     Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
>    
http://www.guycox.net
> -----Original Message-----
> From: Confocal Microscopy List [
[hidden email]
> ] On Behalf Of Rosemary White
> Sent: Wednesday, 18 March 2009 8:45 AM
> To: [hidden email]
> Subject: Re: nail polish
>
> Scott - a great idea re. nail polish!  I usually go down to the $2 
> shop and get 10 of whatever brand's on special (as long as it's 
> colourless).
> cheers,
> Rosemary
>
> Rosemary White
> CSIRO Plant Industry
> GPO Box 1600
> Canberra, ACT 2601
> Australia
>
> ph 61 2 6246 5475
> fx 61 2 6246 5334
>
>
>
> On 18/03/09 4:44 AM, "J. Scott Gens, PhD" <[hidden email]> wrote:
>
>> Valencia-
>>
>> The last time I picked some up nail polish I had such a hard time
>> finding one that did not have some form of plasticizer or other
>> additive in it that gave me serious autofluorescence problems.  I
>> ended up going to the hardware and buying a small container of clear
>> lacquer (usually in the paint/wood stain aisle) instead, and 
>> decanting
>> it into many small nail-polish type bottles. Much cheaper per ounce
>> and weirdly enough in the US at least all ingredients are clearly
>> identified in the hardware product but not on the beauty product.
>>
>> It seems to work fine for fixed samples, caveat is that the solvent 
>> in
>> either product is bad news for live-cell work. For live cells, I'd
>> recommend using "valap", a 1:1:1 homebrew mixture of vaseline,
>> lanolin, and parrafin.
>>
>> Scott
>>
>> --
>> J. Scott Gens, Ph.D.
>> Biocomplexity Institute
>> Indiana University Physics
>> Office:     Room 047H, Simon Interdisciplinary Sciences Bldg.
>> Multi-photon Confocal: 063 Swain Hall West.
>> Bloomington IN 47405
>> 812-855-7946
>> [hidden email]
>>
>>
>>
>> Quoting Valeria Berno <[hidden email]>:
>>
>>> Hi,
>>>
>>> sorry for the practical and banal question....
>>>
>>> Where do you buy the nail polish??at the grocery? if yes, is there 
>>> in
>>> Europe a brand that you can suggest me....
>>> is there any supplier on the web?
>>>
>>>
>>> Thanks
>>>
>>> Valeria
>>>
>
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Keith Morris Keith Morris
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Re: nail polish

In reply to this post by Rosemary.White
We tend to use 'long wearing' and 'double duty' types, generally always
clear, although we have a Goth black that can help with thickness of
application [you can tell if you missed a bit]. Never noticed it bleeding
colour, but you can see when it's been drawn under the cover-slip [less easy
to see that with clear]. It also helps you to avoid the varnish with
immersion oil using our inverted microscopes [we still use clear varnish
mainly though]. The anti-fade mountant we use is generally Vectashield +
DAPI [non setting].

Our brands seem to be Sally Hansen and NYC, chosen probably fairly randomly
from the cosmetics section, rather than buying from say Agar Scientific. I
have used stuff like red engine [car] enamel paint for sealing very thick
specimens under cover-slip [forgotten the name of the product at the
moment].

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford  OX3 7BN,
United Kingdom.

Telephone:  +44 (0)1865 287568
Email:  [hidden email]
Web-pages: http://www.well.ox.ac.uk/cytogenetics/
 

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Rosemary White
Sent: 17 March 2009 23:57
To: [hidden email]
Subject: Re: nail polish

Well, I used to get the colourful ones, then had a batch that leaked colour
into the preparation, so have stuck to the boring clear stuff ever since....


On 18/03/09 10:27 AM, "Maddox Paul" <[hidden email]> wrote:

> I could not agree more!  I like to us different colors, depending on
> my mood of course.
>
> Sent from my iPod
>
> On Mar 17, 2009, at 7:10 PM, "Guy Cox" <[hidden email]> wrote:
>
>> Why use colourless?  I usually use something nice and bright, so
>> that you can see at a glance that the slide is sealed.  It also
>> gives you immediate warning when you are getting close to the edge
>> of the coverslip.  Mind you I do sometimes get funny looks when
>> people see blue and orange nail polish bottles on my desk!
>>
>>                                               Guy
>>
>>
>>
>> Optical Imaging Techniques in Cell Biology
>> by Guy Cox    CRC Press / Taylor & Francis
>>    http://www.guycox.com/optical.htm
>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>> Electron Microscope Unit, Madsen Building F09,
>> University of Sydney, NSW 2006
>> ______________________________________________
>> Phone +61 2 9351 3176     Fax +61 2 9351 7682
>> Mobile 0413 281 861
>> ______________________________________________
>>     http://www.guycox.net
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[hidden email]
>> ] On Behalf Of Rosemary White
>> Sent: Wednesday, 18 March 2009 8:45 AM
>> To: [hidden email]
>> Subject: Re: nail polish
>>
>> Scott - a great idea re. nail polish!  I usually go down to the $2
>> shop and get 10 of whatever brand's on special (as long as it's
>> colourless).
>> cheers,
>> Rosemary
>>
>> Rosemary White
>> CSIRO Plant Industry
>> GPO Box 1600
>> Canberra, ACT 2601
>> Australia
>>
>> ph 61 2 6246 5475
>> fx 61 2 6246 5334
>>
>>
>>
>> On 18/03/09 4:44 AM, "J. Scott Gens, PhD" <[hidden email]> wrote:
>>
>>> Valencia-
>>>
>>> The last time I picked some up nail polish I had such a hard time
>>> finding one that did not have some form of plasticizer or other
>>> additive in it that gave me serious autofluorescence problems.  I
>>> ended up going to the hardware and buying a small container of clear
>>> lacquer (usually in the paint/wood stain aisle) instead, and
>>> decanting
>>> it into many small nail-polish type bottles. Much cheaper per ounce
>>> and weirdly enough in the US at least all ingredients are clearly
>>> identified in the hardware product but not on the beauty product.
>>>
>>> It seems to work fine for fixed samples, caveat is that the solvent
>>> in
>>> either product is bad news for live-cell work. For live cells, I'd
>>> recommend using "valap", a 1:1:1 homebrew mixture of vaseline,
>>> lanolin, and parrafin.
>>>
>>> Scott
>>>
>>> --
>>> J. Scott Gens, Ph.D.
>>> Biocomplexity Institute
>>> Indiana University Physics
>>> Office:     Room 047H, Simon Interdisciplinary Sciences Bldg.
>>> Multi-photon Confocal: 063 Swain Hall West.
>>> Bloomington IN 47405
>>> 812-855-7946
>>> [hidden email]
>>>
>>>
>>>
>>> Quoting Valeria Berno <[hidden email]>:
>>>
>>>> Hi,
>>>>
>>>> sorry for the practical and banal question....
>>>>
>>>> Where do you buy the nail polish??at the grocery? if yes, is there
>>>> in
>>>> Europe a brand that you can suggest me....
>>>> is there any supplier on the web?
>>>>
>>>>
>>>> Thanks
>>>>
>>>> Valeria
>>>>
>>
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>> Checked by AVG.
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>> 16/03/2009 7:01 PM
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>>
>> No virus found in this outgoing message.
>> Checked by AVG.
>> Version: 7.5.557 / Virus Database: 270.11.18/2008 - Release Date:
>> 17/03/2009 4:25 PM
>>