positively charged blocker

[Cialowicz, Katarzyna I]

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Hi All,


I am looking for some positively charged blocker for life cells imaging. Before I've tried BSA solution, but the toxin that I'm using for my experiments attach everywhere, because of the charge.

I would be very grateful If anyone knows about positively charged blocker that is not going to block voltage dependent calcium channels.


Thank you in advance,


Kate



Katarzyna Cialowicz

PhD student
IB3 PG Student Representative

Institute of Biological Chemistry, Biophysics and Bioengineering

Heriot Watt University

Edinburgh EH14 4AS



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[Tony Kowal]

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Is there any chance you could try using poly-L-lysine, or one of the other poly amino acids?  I'm not sure if they would have any effect on your calcium channels.

[Ewa_B]

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Hi Kate,

BSA has some hydrophilic groups on the surface. This may be the source of
your problem.
What about milk? Did you try it?

Eva


--
Eva Biela, Ph.D.
Postdoctoral Research Associate
Basic Medical Sciences
Lyn Hall, 625 Harrison Street
West Lafayette, IN 47907 - 2058
Ph [phone]
www.cyto.purdue.edu


On 15 August 2016 at 14:21, Tony Kowal <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Is there any chance you could try using poly-L-lysine, or one of the other
> poly amino acids?  I'm not sure if they would have any effect on your
> calcium channels.
>

[Stanislav Vitha-2]

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We sometimes use cold water fish skin gelatin (available form Sigma) for blocking in immunocytochemistry.
I am not sure what charge it has.

Stan Vitha
Microscopy and Imaging Center
Texas A&M University