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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I am interested in setting up pre-chirping in the laser path going to a custom built multiphoton. I have been searching in vain for exact information on the components. I bought the prisms, but I am uncertain about the first mirror that directs the laser to the prisms (and then allows the returning, reflected laser to pass through), and the second mirror. My guess is that the first mirror must be a linear polarizer, or a polarizer that separates the s and p polarization. The second mirror must be a grating of some sort that re-polarizes the laser so that it passes through the first mirror. Rather than guessing, it would make my day if I could get specifics on the mirrors. Thanks! Jerry Sedgewick |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Please check an application note (AppsNote29.pdf) at Newport website: http://www.newport.com/store/genproduct.aspx?id=168496&lang=1033&Section=Dow nloads Thanks Newport TAC. Tong Ye, Ph.D. Assistant Research Professor Department of Chemistry Duke University, Box 90346 Durham, NC 27708-0346 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jerry Sedgewick Sent: Wednesday, August 29, 2007 4:01 PM To: [hidden email] Subject: pre-chirping Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I am interested in setting up pre-chirping in the laser path going to a custom built multiphoton. I have been searching in vain for exact information on the components. I bought the prisms, but I am uncertain about the first mirror that directs the laser to the prisms (and then allows the returning, reflected laser to pass through), and the second mirror. My guess is that the first mirror must be a linear polarizer, or a polarizer that separates the s and p polarization. The second mirror must be a grating of some sort that re-polarizes the laser so that it passes through the first mirror. Rather than guessing, it would make my day if I could get specifics on the mirrors. Thanks! Jerry Sedgewick |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Gerry The typical path using 2 prisms is via a small mirror into the first prism (we set this at minimum at minimum deviation but this can be changed to alter chirp). Then to the second prism (at minimum devation to produce a collimated horizontally spread spectrum), and then two mirrors to reflect the light back and displace the beam vertically which then passes back through the second and first prisms respectively. After the beam has left the first prism, note the beam has been displaced vertically is and thereby separated from the input beam. BUT are you sure you need to do this? If the input pulse is ~120 fs the pulse broadening is quite minor. We used a pair of gold mirrors to to return and displace the beam but if you have 1" fs mirrors they would work as well. For typical prism spacing see: Soeller, C. & Cannell, M.B. (1996) Construction of a two-photon microscope and optimisation of illumination pulse width. _Pflugers Archiv_ 432: 555-561. Cheers Mark Jerry Sedgewick wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > I am interested in setting up pre-chirping in the laser path going to > a custom built multiphoton. I have been searching in vain for exact > information on the components. I bought the prisms, but I am uncertain > about the first mirror that directs the laser to the prisms (and then > allows the returning, reflected laser to pass through), and the second > mirror. My guess is that the first mirror must be a linear polarizer, > or a polarizer that separates the s and p polarization. The second > mirror must be a grating of some sort that re-polarizes the laser so > that it passes through the first mirror. > > Rather than guessing, it would make my day if I could get specifics on > the mirrors. > > Thanks! > > Jerry Sedgewick |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Typically the chirp from an average microscopy setup is not enough to greatly diminish image quality. If you are working with weaker samples or otherwise have borderline performance then you may want to try pre-compensation. Otherwise, the complexity and table space involved in setting up a compressor is probably not worth it. |
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