quantitative phase contrast

[Alberto Diaspro]

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Dear
I am intersted in finding papers, including reviews, about the use of (quantitative) phase contrast for chromatin imaging.
Can you help?
Best
Alby

mix.iit.it

[mmodel]

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Quantitative phase (not phase contrast) is a very big field. For example, there is a book

Popescu G. Quantitative Phase Imaging of Cells and Tissues. New York: McGraw-
Hill; 2011. 385 p.

If you specifically need quantitative phase contrast, I only know of one publication:  

Farinas J, Kneen M, Moore M, Verkman AS. Plasma membrane water permeability
of cultured cells and epithelia measured by light microscopy with spatial filtering.
J Gen Physiol 1997;110:283-296.

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alberto Diaspro
Sent: Tuesday, November 28, 2017 9:47 AM
To: [hidden email]
Subject: quantitative phase contrast

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Dear
I am intersted in finding papers, including reviews, about the use of (quantitative) phase contrast for chromatin imaging.
Can you help?
Best
Alby

mix.iit.it

[Peter O'Toole-2]

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Hi Alby

https://www.nature.com/articles/srep02369 may be of interest for QPI in
this area.

Happy to talk more about the potential and having our first own system
installed in 2 weeks time!  It is great for prolonged time-lapse and large
or multiple fields of view, but if you are after fast dynamic studies and
concentrating on a small field of view, there are other options that may be
more suitable (e.g. Tomocube, Phasics etc)

Using the Phasefocus beta-test system, we have gone on to use it for other
applications e.g.  https://www.nature.com/articles/srep22032. The new
system is a step-improvement over the system we used for this.

Best

Pete



On 28 November 2017 at 18:47, MODEL, MICHAEL <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Quantitative phase (not phase contrast) is a very big field. For example,
> there is a book
>
> Popescu G. Quantitative Phase Imaging of Cells and Tissues. New York:
> McGraw-
> Hill; 2011. 385 p.
>
> If you specifically need quantitative phase contrast, I only know of one
> publication:
>
> Farinas J, Kneen M, Moore M, Verkman AS. Plasma membrane water permeability
> of cultured cells and epithelia measured by light microscopy with spatial
> filtering.
> J Gen Physiol 1997;110:283-296.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Alberto Diaspro
> Sent: Tuesday, November 28, 2017 9:47 AM
> To: [hidden email]
> Subject: quantitative phase contrast
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%
> 3Dconfocalmicroscopy&data=01%7C01%7Cmmodel%40KENT.EDU%
> 7Cddce497762ef4f9d6ade08d5368eb0d6%7Ce5a06f4a1ec44d018f73e7dd15f2
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> http%3A%2F%2Fwww.imgur.com&data=01%7C01%7Cmmodel%40KENT.EDU%
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> gg%3D&reserved=0 and include the link in your posting.
> *****
>
> Dear
> I am intersted in finding papers, including reviews, about the use of
> (quantitative) phase contrast for chromatin imaging.
> Can you help?
> Best
> Alby
>
> mix.iit.it
>

--
Latest Publication: Correlative super-resolution fluorescence and electron
microscopy using conventional fluorescent proteins in vacuo.
<http://www.sciencedirect.com/science/article/pii/S104784771730093X>


<http://www.linkedin.com/pub/peter-o-toole/1b/b79/444>
Dr Peter O'Toole
Director of the Bioscience Technology Facility
Head of Imaging and Cytometry
Bioscience Technology Facility
Department of Biology (Area 15)
University of York
YORK
YO10 5DD

Tel : [phone]
email : [hidden email]
www.york.ac.uk/biology/tf

EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email.htm

[mmodel]

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Quantitative phase information can also be obtained from a pair of mutually defocused bright-field images (Calibrated brightfield-based imaging for measuring intracellular protein concentration. Cytometry DOI: 10.1002/cyto.a.23145)

Mike

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Peter O'Toole
Sent: Wednesday, November 29, 2017 8:06 AM
To: [hidden email]
Subject: Re: quantitative phase contrast

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Hi Alby

https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep02369&data=01%7C01%7Cmmodel%40KENT.EDU%7Cbc6191e7236c439ed45608d5372a0882%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1&sdata=WMmA9cG37o6xPUQlFaTGmDOabm%2FmT2tHk56Q1V6mck0%3D&reserved=0 may be of interest for QPI in this area.

Happy to talk more about the potential and having our first own system installed in 2 weeks time!  It is great for prolonged time-lapse and large or multiple fields of view, but if you are after fast dynamic studies and concentrating on a small field of view, there are other options that may be more suitable (e.g. Tomocube, Phasics etc)

Using the Phasefocus beta-test system, we have gone on to use it for other applications e.g.  https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep22032&data=01%7C01%7Cmmodel%40KENT.EDU%7Cbc6191e7236c439ed45608d5372a0882%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1&sdata=4t%2BH9VJEBLghz13hrrKubgqBQyOU1gn6XyHJMYqq4%2BE%3D&reserved=0. The new system is a step-improvement over the system we used for this.

Best

Pete



On 28 November 2017 at 18:47, MODEL, MICHAEL <[hidden email]> wrote:

> *****
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> 3D&reserved=0 Post images on
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> *****
>
> Quantitative phase (not phase contrast) is a very big field. For
> example, there is a book
>
> Popescu G. Quantitative Phase Imaging of Cells and Tissues. New York:
> McGraw-
> Hill; 2011. 385 p.
>
> If you specifically need quantitative phase contrast, I only know of
> one
> publication:
>
> Farinas J, Kneen M, Moore M, Verkman AS. Plasma membrane water
> permeability of cultured cells and epithelia measured by light
> microscopy with spatial filtering.
> J Gen Physiol 1997;110:283-296.
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]]
> On Behalf Of Alberto Diaspro
> Sent: Tuesday, November 28, 2017 9:47 AM
> To: [hidden email]
> Subject: quantitative phase contrast
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://na01.safelinks.protection.outlook.com/?url=
> http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%
> 3Dconfocalmicroscopy&data=01%7C01%7Cmmodel%40KENT.EDU%
> 7Cddce497762ef4f9d6ade08d5368eb0d6%7Ce5a06f4a1ec44d018f73e7dd15f2
> 6134%7C1&sdata=dVBH9Yjq3yEf7hss9L1AUIZn7eJA5YdqlWVzpgZuNKY%3D&reserved
> =0 Post images on https://na01.safelinks.protection.outlook.com/?url=
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> gg%3D&reserved=0 and include the link in your posting.
> *****
>
> Dear
> I am intersted in finding papers, including reviews, about the use of
> (quantitative) phase contrast for chromatin imaging.
> Can you help?
> Best
> Alby
>
> mix.iit.it
>

--
Latest Publication: Correlative super-resolution fluorescence and electron microscopy using conventional fluorescent proteins in vacuo.
<https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Farticle%2Fpii%2FS104784771730093X&data=01%7C01%7Cmmodel%40KENT.EDU%7Cbc6191e7236c439ed45608d5372a0882%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1&sdata=u6Q9vcww%2B7HFuvg9ADP18rjAf92Fi7AM3rgPqH%2BiMkg%3D&reserved=0>


<https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.linkedin.com%2Fpub%2Fpeter-o-toole%2F1b%2Fb79%2F444&data=01%7C01%7Cmmodel%40KENT.EDU%7Cbc6191e7236c439ed45608d5372a0882%7Ce5a06f4a1ec44d018f73e7dd15f26134%7C1&sdata=r4KGfLwoWddp1TUr6NDCEN%2FICtbCcivsvKHtxgJdfLg%3D&reserved=0>
Dr Peter O'Toole
Director of the Bioscience Technology Facility
Head of Imaging and Cytometry
Bioscience Technology Facility
Department of Biology (Area 15)
University of York
YORK
YO10 5DD

Tel : [phone]
email : [hidden email]
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[Sachiko.Sato]

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It is possible to use DIC to perform series of quantitative analysis of cell behaviours, including cell division etc.

https://www.nature.com/articles/srep23328 <https://www.nature.com/articles/srep23328>
https://www.biorxiv.org/content/early/2017/11/07/215764


We have also developed software that performs this analysis in a semi-automatic manner.
(We will report this software system early next year.)

Sachiko

*****************************************************************
Sachiko Sato, Ph. D.
Professor
  Glycobiology and Bioimaging laboratory,
  Research Centre for Infectious Diseases,
  Faculty of Medicine, Laval University, Quebec
Director of  Bioimaging platform
  RC-709 CHUL-CHU de Québec Research Centre,
  2705 boul, Laurier, Quebec,
  QC, Canada G1V 4G2
  Tel:[phone] ext 48647
  Fax:[phone]
[hidden email]
*****************************************************************

> On Nov 29, 2017, at 8:06 AM, Peter O'Toole <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Alby
>
> https://www.nature.com/articles/srep02369 may be of interest for QPI in
> this area.
>
> Happy to talk more about the potential and having our first own system
> installed in 2 weeks time!  It is great for prolonged time-lapse and large
> or multiple fields of view, but if you are after fast dynamic studies and
> concentrating on a small field of view, there are other options that may be
> more suitable (e.g. Tomocube, Phasics etc)
>
> Using the Phasefocus beta-test system, we have gone on to use it for other
> applications e.g.  https://www.nature.com/articles/srep22032. The new
> system is a step-improvement over the system we used for this.
>
> Best
>
> Pete
>
>
>
> On 28 November 2017 at 18:47, MODEL, MICHAEL <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Quantitative phase (not phase contrast) is a very big field. For example,
>> there is a book
>>
>> Popescu G. Quantitative Phase Imaging of Cells and Tissues. New York:
>> McGraw-
>> Hill; 2011. 385 p.
>>
>> If you specifically need quantitative phase contrast, I only know of one
>> publication:
>>
>> Farinas J, Kneen M, Moore M, Verkman AS. Plasma membrane water permeability
>> of cultured cells and epithelia measured by light microscopy with spatial
>> filtering.
>> J Gen Physiol 1997;110:283-296.
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[hidden email]]
>> On Behalf Of Alberto Diaspro
>> Sent: Tuesday, November 28, 2017 9:47 AM
>> To: [hidden email]
>> Subject: quantitative phase contrast
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> https://na01.safelinks.protection.outlook.com/?url=
>> http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%
>> 3Dconfocalmicroscopy&data=01%7C01%7Cmmodel%40KENT.EDU%
>> 7Cddce497762ef4f9d6ade08d5368eb0d6%7Ce5a06f4a1ec44d018f73e7dd15f2
>> 6134%7C1&sdata=dVBH9Yjq3yEf7hss9L1AUIZn7eJA5YdqlWVzpgZuNKY%3D&reserved=0
>> Post images on https://na01.safelinks.protection.outlook.com/?url=
>> http%3A%2F%2Fwww.imgur.com&data=01%7C01%7Cmmodel%40KENT.EDU%
>> 7Cddce497762ef4f9d6ade08d5368eb0d6%7Ce5a06f4a1ec44d018f73e7dd15f2
>> 6134%7C1&sdata=cY%2FDl4RYpOIx%2Fh5HEZfmPP5stMf5toIKOmV9PGJut
>> gg%3D&reserved=0 and include the link in your posting.
>> *****
>>
>> Dear
>> I am intersted in finding papers, including reviews, about the use of
>> (quantitative) phase contrast for chromatin imaging.
>> Can you help?
>> Best
>> Alby
>>
>> mix.iit.it
>>
>
>
>
> --
> Latest Publication: Correlative super-resolution fluorescence and electron
> microscopy using conventional fluorescent proteins in vacuo.
> <http://www.sciencedirect.com/science/article/pii/S104784771730093X>
>
>
> <http://www.linkedin.com/pub/peter-o-toole/1b/b79/444>
> Dr Peter O'Toole
> Director of the Bioscience Technology Facility
> Head of Imaging and Cytometry
> Bioscience Technology Facility
> Department of Biology (Area 15)
> University of York
> YORK
> YO10 5DD
>
> Tel : [phone]
> email : [hidden email]
> www.york.ac.uk/biology/tf
>
> EMAIL DISCLAIMER http://www.york.ac.uk/docs/disclaimer/email.htm

[Tim Feinstein]

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Hi Alby,

I don’t know what type of quantitation you have in mind, but Conly Reider at SUNY Albany has an extensive body of work on phase and DIC analysis of chromatin.  For example Mol. Biol. Cell 12:4054, but the full list is pretty long.  

Best,


Tim

Timothy Feinstein, Ph.D.



On 11/28/17, 1:34 PM, "Confocal Microscopy List on behalf of Alberto Diaspro" <[hidden email] on behalf of [hidden email]> wrote:

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    *****
   
    Dear
    I am intersted in finding papers, including reviews, about the use of (quantitative) phase contrast for chromatin imaging.
    Can you help?
    Best
    Alby
   
    mix.iit.it
   

[Catalin Chiritescu]

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Hi Alberto,

I will supplement the book info from Mike Model with three papers that illustrate the quantitative phase imaging field:
DOI: 10.1063/PT.3.3553 (general principles)
DOI: 10.1038/NPHOTON.2013.35 (3D structure of live cells)
DOI: 10.1021/acsnano.6b06945 (microtubules)

We measured chromatin condensation in sperm cells as a function of labile Zn content for BIF Northwestern University (O'Halloran group). We used a Spatial Light Interference Microscopy (SLIM) module connected to a regular white light inverted optical microscope. Feel free to contact off-line to see a summary of the results. SLIM measures protein content as dry mass and can also do refractometry - with prolonged timelapse and large fields of view.

A new system is going live at Politecnico di Torino next year for automated multichannel 4D imaging of live cells and tissues. We can connect you to the PI so you can collaborate if you are interested.

And to keep things fair, here is the tag for my reply.

******COMMERCIAL********

[Stephen Cody-2]

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G'day Alby,

You may be interested in this paper:

CODY, S. H., XIANG, S. D., LAYTON, M. J., HANDMAN, E., LAM, M. H. C.,
LAYTON, J. E., NICE, E. C. and HEATH, J. K. (2005), A simple method
allowing DIC imaging in conjunction with confocal microscopy. Journal of
Microscopy, 217: 265–274. doi:10.1111/j.1365-2818.2005.01452.x

All the best,
Steve

On 29 November 2017 at 01:47, Alberto Diaspro <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear
> I am intersted in finding papers, including reviews, about the use of
> (quantitative) phase contrast for chromatin imaging.
> Can you help?
> Best
> Alby
>
> mix.iit.it
>

--
Stephen H. Cody