question about laser
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Hi everyone,
We are looking for a coherent (or continuous wave) laser ranging from 380nm to 400nm. So far we haven't found anything impressive. Does anyone know any laser between that range? Thanks a lot! Yue |
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A CW laser is by definition narrowband; there's no way you can have one with 20nm spectral bandwidth. The only thing I can think of off hand that would give you the spectral range you are looking for is an LED. The only other alternative would be something like a supercontinuum (or so-called 'white' laser) but that emits over a huge range and would be overkill. I'd recommend trying to find a really powerful LED in that range, or even a bunch of lower-powered LEDs bundled into an array.
Craig On Wed, Mar 4, 2009 at 10:43 AM, <[hidden email]> wrote: Hi everyone, |
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Ah, just realized I misunderstood your question. You just need a laser line between 380 and 400. Your best bet there would be to get a high-powered IR diode laser and use a crystal to double it. A diode laser at 780nm doubled would give you 390nm.
Craig On Wed, Mar 4, 2009 at 11:49 AM, Craig Brideau <[hidden email]> wrote: A CW laser is by definition narrowband; there's no way you can have one with 20nm spectral bandwidth. The only thing I can think of off hand that would give you the spectral range you are looking for is an LED. The only other alternative would be something like a supercontinuum (or so-called 'white' laser) but that emits over a huge range and would be overkill. I'd recommend trying to find a really powerful LED in that range, or even a bunch of lower-powered LEDs bundled into an array. |
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Speaking of which, whatever happened to the promised solid state
laser that is basically the same as the 405 nm but tuned to emit as
short as 385 nm? I may be a bit off, but the DAPI fluorescence
emission is about 7 times higher at 385 nm (~45% max) compared to 405
nm (~6% max).
Mario
Ah, just realized I misunderstood your question. You just need a laser line between 380 and 400. Your best bet there would be to get a high-powered IR diode laser and use a crystal to double it. A diode laser at 780nm doubled would give you 390nm. On Wed, Mar 4, 2009 at 11:49 AM, Craig Brideau <[hidden email]> wrote: -- ________________________________________________________________________________
Mario M. Moronne, Ph.D. [hidden email] [hidden email] [hidden email] |
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Sorry for the confusion. I wasn't clear enough about my question. You
are right, Craig. I'm looking for a laser line that's between 380nm and 400nm. Thank you all for the good suggestions! Yue Quoting Mario <[hidden email]>: > Speaking of which, whatever happened to the promised solid state laser > that is basically the same as the 405 nm but tuned to emit as short as > 385 nm? I may be a bit off, but the DAPI fluorescence emission is about > 7 times higher at 385 nm (~45% max) compared to 405 nm (~6% max). > > Mario > >> Ah, just realized I misunderstood your question. You just need a >> laser line between 380 and 400. Your best bet there would be to >> get a high-powered IR diode laser and use a crystal to double it. >> A diode laser at 780nm doubled would give you 390nm. >> >> Craig >> >> On Wed, Mar 4, 2009 at 11:49 AM, Craig Brideau >> <<mailto:[hidden email]>[hidden email]> wrote: >> >> A CW laser is by definition narrowband; there's no way you can have >> one with 20nm spectral bandwidth. The only thing I can think of >> off hand that would give you the spectral range you are looking for >> is an LED. The only other alternative would be something like a >> supercontinuum (or so-called 'white' laser) but that emits over a >> huge range and would be overkill. I'd recommend trying to find a >> really powerful LED in that range, or even a bunch of lower-powered >> LEDs bundled into an array. >> >> Craig >> >> >> >> On Wed, Mar 4, 2009 at 10:43 AM, >> <<mailto:[hidden email]>[hidden email]> wrote: >> >> Hi everyone, >> >> We are looking for a coherent (or continuous wave) laser ranging >> from 380nm to 400nm. So far we haven't found anything impressive. >> Does anyone know any laser between that range? Thanks a lot! >> >> Yue > > > -- > ________________________________________________________________________________ > Mario M. Moronne, Ph.D. > > [hidden email] > [hidden email] > [hidden email] |
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In reply to this post by yue_li
Second Harmonic of CW Ti:Sapphire tunable between 760 and 800 nm.
Best regards, Sergey Egorov Del Mar Photonics 4119 Twilight Ridge San Diego, CA 92130 tel (858) 876-3133 fax (858) 630-2376 http://www.dmphotonics.com/ [hidden email] wrote: > Hi everyone, > > We are looking for a coherent (or continuous wave) laser ranging from > 380nm to 400nm. So far we haven't found anything impressive. Does > anyone know any laser between that range? Thanks a lot! > > Yue > |
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In reply to this post by yue_li
Picoquant supply diode lasers (pulsed or cw) from 375nm
to something like 470nm (you can get whatever you want.) I have no connection with the company (except as a satisfied customer). I'm sure there are other suppliers as well. They are quite cheap (a tiny fraction of a frequency doubled TiS). You do have to think a bit about your optics at this wavelength (and how you align a beam you can't see). Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 http://www.guycox.net ______________________________________________ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email] Sent: Thursday, 5 March 2009 10:48 AM To: [hidden email] Subject: Re: question about laser Sorry for the confusion. I wasn't clear enough about my question. You are right, Craig. I'm looking for a laser line that's between 380nm and 400nm. Thank you all for the good suggestions! Yue Quoting Mario <[hidden email]>: > Speaking of which, whatever happened to the promised solid state laser > that is basically the same as the 405 nm but tuned to emit as short as > 385 nm? I may be a bit off, but the DAPI fluorescence emission is about > 7 times higher at 385 nm (~45% max) compared to 405 nm (~6% max). > > Mario > >> Ah, just realized I misunderstood your question. You just need a >> laser line between 380 and 400. Your best bet there would be to >> get a high-powered IR diode laser and use a crystal to double it. >> A diode laser at 780nm doubled would give you 390nm. >> >> Craig >> >> On Wed, Mar 4, 2009 at 11:49 AM, Craig Brideau >> <<mailto:[hidden email]>[hidden email]> wrote: >> >> A CW laser is by definition narrowband; there's no way you can have >> one with 20nm spectral bandwidth. The only thing I can think of >> off hand that would give you the spectral range you are looking for >> is an LED. The only other alternative would be something like a >> supercontinuum (or so-called 'white' laser) but that emits over a >> huge range and would be overkill. I'd recommend trying to find a >> really powerful LED in that range, or even a bunch of lower-powered >> LEDs bundled into an array. >> >> Craig >> >> >> >> On Wed, Mar 4, 2009 at 10:43 AM, >> <<mailto:[hidden email]>[hidden email]> wrote: >> >> Hi everyone, >> >> We are looking for a coherent (or continuous wave) laser ranging >> from 380nm to 400nm. So far we haven't found anything impressive. >> Does anyone know any laser between that range? Thanks a lot! >> >> Yue > > > -- > ________ > Mario M. Moronne, Ph.D. > > [hidden email] > [hidden email] > [hidden email] No virus found in this incoming message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: 4/03/2009 7:41 AM |
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Thank you so much for the information. Cost is a big factor in our
decision. I'll check that company out. We can't align the laser in the lab, but we have someone else help us to do it. Yue Quoting Guy Cox <[hidden email]>: > Picoquant supply diode lasers (pulsed or cw) from 375nm > to something like 470nm (you can get whatever you want.) > I have no connection with the company (except as a > satisfied customer). I'm sure there are other suppliers > as well. They are quite cheap (a tiny fraction of a > frequency doubled TiS). You do have to think a bit about > your optics at this wavelength (and how you align a beam > you can't see). > > Guy > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Electron Microscope Unit, Madsen Building F09, > University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 http://www.guycox.net > ______________________________________________ > > > -----Original Message----- > From: Confocal Microscopy List [mailto:[hidden email]] > On Behalf Of [hidden email] > Sent: Thursday, 5 March 2009 10:48 AM > To: [hidden email] > Subject: Re: question about laser > > Sorry for the confusion. I wasn't clear enough about my question. You > are right, Craig. I'm looking for a laser line that's between 380nm > and 400nm. > > Thank you all for the good suggestions! > > Yue > > > Quoting Mario <[hidden email]>: > >> Speaking of which, whatever happened to the promised solid state laser >> that is basically the same as the 405 nm but tuned to emit as short as >> 385 nm? I may be a bit off, but the DAPI fluorescence emission is > about >> 7 times higher at 385 nm (~45% max) compared to 405 nm (~6% max). >> >> Mario >> >>> Ah, just realized I misunderstood your question. You just need a >>> laser line between 380 and 400. Your best bet there would be to >>> get a high-powered IR diode laser and use a crystal to double it. >>> A diode laser at 780nm doubled would give you 390nm. >>> >>> Craig >>> >>> On Wed, Mar 4, 2009 at 11:49 AM, Craig Brideau >>> <<mailto:[hidden email]>[hidden email]> wrote: >>> >>> A CW laser is by definition narrowband; there's no way you can have >>> one with 20nm spectral bandwidth. The only thing I can think of >>> off hand that would give you the spectral range you are looking for >>> is an LED. The only other alternative would be something like a >>> supercontinuum (or so-called 'white' laser) but that emits over a >>> huge range and would be overkill. I'd recommend trying to find a >>> really powerful LED in that range, or even a bunch of lower-powered >>> LEDs bundled into an array. >>> >>> Craig >>> >>> >>> >>> On Wed, Mar 4, 2009 at 10:43 AM, >>> <<mailto:[hidden email]>[hidden email]> wrote: >>> >>> Hi everyone, >>> >>> We are looking for a coherent (or continuous wave) laser ranging >>> from 380nm to 400nm. So far we haven't found anything impressive. >>> Does anyone know any laser between that range? Thanks a lot! >>> >>> Yue >> >> >> -- >> > ________________________________________________________________________ > ________ >> Mario M. Moronne, Ph.D. >> >> [hidden email] >> [hidden email] >> [hidden email] > > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: > 4/03/2009 7:41 AM > > |
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In reply to this post by femto@sciner.com
I don't quite understand. The laser beam we are looking for is between
380 and 400nm. Are you thinking of the doubler? Could you please give more detail? Thanks a lot. Yue Quoting "Femto @Sciner" <[hidden email]>: > Second Harmonic of CW Ti:Sapphire tunable between 760 and 800 nm. > > Best regards, > Sergey Egorov > Del Mar Photonics > 4119 Twilight Ridge > San Diego, CA 92130 > tel (858) 876-3133 > fax (858) 630-2376 > http://www.dmphotonics.com/ > > > [hidden email] wrote: >> Hi everyone, >> >> We are looking for a coherent (or continuous wave) laser ranging >> from 380nm to 400nm. So far we haven't found anything impressive. >> Does anyone know any laser between that range? Thanks a lot! >> >> Yue >> |
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Yes, he was referring to a doubler. The problem is people are
still not clear whether you want to tune the wavelength between 380 and 400 nm (in which case Sergey's suggestion is the most practical way to go, but is very expensive) or whether you just want one fixed wavelength within that range, which is easy and cheap to get with a diode. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email] Sent: Friday, 6 March 2009 4:18 AM To: [hidden email] Subject: Re: question about laser I don't quite understand. The laser beam we are looking for is between 380 and 400nm. Are you thinking of the doubler? Could you please give more detail? Thanks a lot. Yue Quoting "Femto @Sciner" <[hidden email]>: > Second Harmonic of CW Ti:Sapphire tunable between 760 and 800 nm. > > Best regards, > Sergey Egorov > Del Mar Photonics > 4119 Twilight Ridge > San Diego, CA 92130 > tel (858) 876-3133 > fax (858) 630-2376 > http://www.dmphotonics.com/ > > > [hidden email] wrote: >> Hi everyone, >> >> We are looking for a coherent (or continuous wave) laser ranging >> from 380nm to 400nm. So far we haven't found anything impressive. >> Does anyone know any laser between that range? Thanks a lot! >> >> Yue >> No virus found in this incoming message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: 4/03/2009 7:41 AM No virus found in this outgoing message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: 4/03/2009 7:41 AM |
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I just want one fixed wavelength within that range eventually. The
problem is that I don't know which one will work the best on my sample. I only know it should lay between 380 and 400nm from my other experiments. And I'm hoping to find an inexpensive way to find out. Yue Quoting Guy Cox <[hidden email]>: > Yes, he was referring to a doubler. The problem is people are > still not clear whether you want to tune the wavelength between > 380 and 400 nm (in which case Sergey's suggestion is the most > practical way to go, but is very expensive) or whether you just > want one fixed wavelength within that range, which is easy and > cheap to get with a diode. > > Guy > > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Electron Microscope Unit, Madsen Building F09, > University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of > [hidden email] > Sent: Friday, 6 March 2009 4:18 AM > To: [hidden email] > Subject: Re: question about laser > > I don't quite understand. The laser beam we are looking for is > between 380 and 400nm. Are you thinking of the doubler? Could you > please give more detail? Thanks a lot. > > Yue > > > Quoting "Femto @Sciner" <[hidden email]>: > >> Second Harmonic of CW Ti:Sapphire tunable between 760 and 800 nm. >> >> Best regards, >> Sergey Egorov >> Del Mar Photonics >> 4119 Twilight Ridge >> San Diego, CA 92130 >> tel (858) 876-3133 >> fax (858) 630-2376 >> http://www.dmphotonics.com/ >> >> >> [hidden email] wrote: >>> Hi everyone, >>> >>> We are looking for a coherent (or continuous wave) laser ranging >>> from 380nm to 400nm. So far we haven't found anything impressive. >>> Does anyone know any laser between that range? Thanks a lot! >>> >>> Yue >>> > > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: > 4/03/2009 7:41 AM > > > No virus found in this outgoing message. > Checked by AVG. > Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: > 4/03/2009 7:41 AM > > |
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Yue,
Please, feel free to tell us a little bit more details than you currently are providing. There is a wealth of information on this list, and with more information from you comes more knowledge from the group. What is your application? It is potentially a cheaper and more practical solution to buy the best laser you can afford that fits the needs of your fluorophore, and then purchase custom dichroic mirrors and bandpass filters if necessary. These can be more affordable to buy than new lasers, and can be custom manufactured to meet your experimental needs from a few companies. Chroma provides this service in a timely fashion. (no commercial interest) -- Samuel A. Connell Director of Light Microscopy Cell & Tissue Imaging Center St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105-3678 Office (901) 495-2536 Cell (901) 603-3162 [hidden email] On 3/5/09 9:47 PM, "[hidden email]" <[hidden email]> wrote: I just want one fixed wavelength within that range eventually. The problem is that I don't know which one will work the best on my sample. I only know it should lay between 380 and 400nm from my other experiments. And I'm hoping to find an inexpensive way to find out. Yue Quoting Guy Cox <[hidden email]>: > Yes, he was referring to a doubler. The problem is people are > still not clear whether you want to tune the wavelength between > 380 and 400 nm (in which case Sergey's suggestion is the most > practical way to go, but is very expensive) or whether you just > want one fixed wavelength within that range, which is easy and > cheap to get with a diode. > > Guy > > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Electron Microscope Unit, Madsen Building F09, > University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of > [hidden email] > Sent: Friday, 6 March 2009 4:18 AM > To: [hidden email] > Subject: Re: question about laser > > I don't quite understand. The laser beam we are looking for is > between 380 and 400nm. Are you thinking of the doubler? Could you > please give more detail? Thanks a lot. > > Yue > > > Quoting "Femto @Sciner" <[hidden email]>: > >> Second Harmonic of CW Ti:Sapphire tunable between 760 and 800 nm. >> >> Best regards, >> Sergey Egorov >> Del Mar Photonics >> 4119 Twilight Ridge >> San Diego, CA 92130 >> tel (858) 876-3133 >> fax (858) 630-2376 >> http://www.dmphotonics.com/ >> >> >> [hidden email] wrote: >>> Hi everyone, >>> >>> We are looking for a coherent (or continuous wave) laser ranging >>> from 380nm to 400nm. So far we haven't found anything impressive. >>> Does anyone know any laser between that range? Thanks a lot! >>> >>> Yue >>> > > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: > 4/03/2009 7:41 AM > > > No virus found in this outgoing message. > Checked by AVG. > Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: > 4/03/2009 7:41 AM > > ________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer |
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In reply to this post by yue_li
Can't you just check your fluorochrome on a spectrofluorimeter?
If for some reason you actually need to form images, why not use a tunable filter with a mercury or metal halide lamp? (Or even just buy a few different excitation filters if a tunable filter is too dear.) Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of [hidden email] Sent: Friday, 6 March 2009 2:48 PM To: [hidden email] Subject: Re: question about laser I just want one fixed wavelength within that range eventually. The problem is that I don't know which one will work the best on my sample. I only know it should lay between 380 and 400nm from my other experiments. And I'm hoping to find an inexpensive way to find out. Yue Quoting Guy Cox <[hidden email]>: > Yes, he was referring to a doubler. The problem is people are still > not clear whether you want to tune the wavelength between 380 and 400 > nm (in which case Sergey's suggestion is the most practical way to > go, but is very expensive) or whether you just want one fixed > wavelength within that range, which is easy and cheap to get with a > diode. > > Guy > > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, > Madsen Building F09, University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of > [hidden email] > Sent: Friday, 6 March 2009 4:18 AM > To: [hidden email] > Subject: Re: question about laser > > I don't quite understand. The laser beam we are looking for is > between 380 and 400nm. Are you thinking of the doubler? Could you > please give more detail? Thanks a lot. > > Yue > > > Quoting "Femto @Sciner" <[hidden email]>: > >> Second Harmonic of CW Ti:Sapphire tunable between 760 and 800 nm. >> >> Best regards, >> Sergey Egorov >> Del Mar Photonics >> 4119 Twilight Ridge >> San Diego, CA 92130 >> tel (858) 876-3133 >> fax (858) 630-2376 >> http://www.dmphotonics.com/ >> >> >> [hidden email] wrote: >>> Hi everyone, >>> >>> We are looking for a coherent (or continuous wave) laser ranging >>> from 380nm to 400nm. So far we haven't found anything impressive. >>> Does anyone know any laser between that range? Thanks a lot! >>> >>> Yue >>> > > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: > 4/03/2009 7:41 AM > > > No virus found in this outgoing message. > Checked by AVG. > Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: > 4/03/2009 7:41 AM > > No virus found in this incoming message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: 4/03/2009 7:41 AM No virus found in this outgoing message. Checked by AVG. Version: 7.5.557 / Virus Database: 270.11.7/1983 - Release Date: 4/03/2009 7:41 AM |
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