recurring contamination of MP cooling

[Ekaterina PAPUSHEVA-2]

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Dear all,

Need your advice regarding the contamination of our MP cooling.

We were filling our ThermoTek chillers (T255P) that are connected with Cameleon II MP lasers with  distilled water, according to Coherent instructions.
Since few days, we got dramatic contamination (some algae or bacteria growing) in the water.

Even if we flash a chiller with 10 l water and refill new, we get already some visible contamination next day. One of the systems was clogged once.

Are there any tips of how to get the growth out?
We thought of adding 5% ethanol instead of water. Or building bacterial filters (like on FACS machines) in the flow circle.

Does someone have experience with these two approaches, and is there something better?

Looking forward to many helpful tips! Nice weekend to everyone, except those who have to flush their chillers :)

Ekaterina

___

Ekaterina Papusheva,
Imaging Facility Manager

Institute of Science and Technology Austria

Phone  [phone];
Mobile [phone]
Fax    [phone]

Am Campus 1

A-3400 Klosterneuburg

Visit our website: www.ist.ac.at<http://www.ist.ac.at>


This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.

[Dan Focht]

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Ekatrina


I am not sure about ThermoTek brand chillers but we use a 10% alcohol and water in our recirculating chiller and have never had anything grow in it.


Dan





On Oct 7, 2011, at 11:05 AM, Ekaterina PAPUSHEVA wrote:

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Dear all,

Need your advice regarding the contamination of our MP cooling.

We were filling our ThermoTek chillers (T255P) that are connected with Cameleon II MP lasers with  distilled water, according to Coherent instructions.
Since few days, we got dramatic contamination (some algae or bacteria growing) in the water.

Even if we flash a chiller with 10 l water and refill new, we get already some visible contamination next day. One of the systems was clogged once.

Are there any tips of how to get the growth out?
We thought of adding 5% ethanol instead of water. Or building bacterial filters (like on FACS machines) in the flow circle.

Does someone have experience with these two approaches, and is there something better?

Looking forward to many helpful tips! Nice weekend to everyone, except those who have to flush their chillers :)

Ekaterina

___

Ekaterina Papusheva,
Imaging Facility Manager

Institute of Science and Technology Austria

Phone  [phone];
Mobile [phone]
Fax    [phone]

Am Campus 1

A-3400 Klosterneuburg

Visit our website: www.ist.ac.at<http://www.ist.ac.at>


This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.

Dan Focht
Bioptechs, Inc.
3560 Beck Rd.
Butler, PA 16002
www.bioptechs.com
P: [phone]
F: [phone]
[hidden email]

[Gary G. Li-2]

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Hi Ekaterina,

You need to add 10% OptiShield Plus (Opti Temp, Inc.
www.optishield.net) in the distilled water to prevent biological
growth in the coolant line.  OptiShield Plus protects against both
galvanic corrosion and biological buildup.

OptiShield Plus, P/N 400113, 1 Pint Size; P/N 400115, 1 Gallon Size.

Flush the coolant line more frequently until it becomes normal. Then
make coolant change monthly afterwards.

Gary G Li, PhD
Microscopy Facility Manager
NIH/NIDA at Baltimore
MD 21224, USA


On Fri, Oct 7, 2011 at 12:00 PM, Dan Focht <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Ekatrina
>
>
> I am not sure about ThermoTek brand chillers but we use a 10% alcohol and water in our recirculating chiller and have never had anything grow in it.
>
>
> Dan
>
>
>
>
>
> On Oct 7, 2011, at 11:05 AM, Ekaterina PAPUSHEVA wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear all,
>
> Need your advice regarding the contamination of our MP cooling.
>
> We were filling our ThermoTek chillers (T255P) that are connected with Cameleon II MP lasers with  distilled water, according to Coherent instructions.
> Since few days, we got dramatic contamination (some algae or bacteria growing) in the water.
>
> Even if we flash a chiller with 10 l water and refill new, we get already some visible contamination next day. One of the systems was clogged once.
>
> Are there any tips of how to get the growth out?
> We thought of adding 5% ethanol instead of water. Or building bacterial filters (like on FACS machines) in the flow circle.
>
> Does someone have experience with these two approaches, and is there something better?
>
> Looking forward to many helpful tips! Nice weekend to everyone, except those who have to flush their chillers :)
>
> Ekaterina
>
> ___
>
> Ekaterina Papusheva,
> Imaging Facility Manager
>
> Institute of Science and Technology Austria
>
> Phone  [phone];
> Mobile [phone]
> Fax    [phone]
>
> Am Campus 1
>
> A-3400 Klosterneuburg
>
> Visit our website: www.ist.ac.at<http://www.ist.ac.at>
>
>
> This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.
>
> Dan Focht
> Bioptechs, Inc.
> 3560 Beck Rd.
> Butler, PA 16002
> www.bioptechs.com
> P: [phone]
> F: [phone]
> [hidden email]
>

[Oshel, Philip Eugene]

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Ekaterina,

What is the plumbing for the chiller lines? Opaque hose or
transparent? Polymer hose or metal pipes?
If transparent polymer, then maybe you do have algae or bacteria,
possibly with opaque hose. Check with the chiller manufacturer, but I
have had success with biocontamination in chiller lines by running
about 1/2 a filter-holder's worth of bleach through the lines, then
rinse with water. BUT! check first - this can be a horrible idea.

If there are metal pipes anywhere in the system, and 2 different
metals are in contact, like copper lines connecting to the coils of
the chiller, even if polymer hoses are used otherwise, then you could
be getting electrolytic corrosion. This can occur even if you don't
see any obvious metal connections (what's inside the chiller?). The
simplest thing is to put some of the green gunk under a microscope
and look. If you have access to an SEM with EDS (x-ray spectroscopy),
you can get a quick analysis of the stuff.

Also, check the distilled water and the manufacturer's requirements.
Distilled water is usually acidic (from atmospheric CO2 uptake) which
can cause problems, and is also know as "hungry water" (as is
deionized water). The lack of ions in the water, especially metals,
causes the water to "pull" metal ions out of the pipes to get some
chemical equilibrium. This isn't a problem for systems specifically
designed for distilled water, but can be for systems that aren't so
designed.

Phil

>Dear all,
>
>Need your advice regarding the contamination of our MP cooling.
>
>We were filling our ThermoTek chillers (T255P) that are connected
>with Cameleon II MP lasers with  distilled water, according to
>Coherent instructions.
>Since few days, we got dramatic contamination (some algae or
>bacteria growing) in the water.
>
>Even if we flash a chiller with 10 l water and refill new, we get
>already some visible contamination next day. One of the systems was
>clogged once.
>
>Are there any tips of how to get the growth out?
>We thought of adding 5% ethanol instead of water. Or building
>bacterial filters (like on FACS machines) in the flow circle.
>
>Does someone have experience with these two approaches, and is there
>something better?
>
>Looking forward to many helpful tips! Nice weekend to everyone,
>except those who have to flush their chillers :)
>
>Ekaterina
>
>___
>
>Ekaterina Papusheva,
>Imaging Facility Manager
>
>Institute of Science and Technology Austria

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
[phone]

[Armstrong, Brian]

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To join, leave or search the confocal microscopy listserv, go to:
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*****

Hi, I concur with Gary. We use Optishield and change the distilled water monthly. Note; this is not milliQ or DDH2O.

I wish that our first Coherent service rep would have told me this. We had similar issues with clogging etc... and had to replace the cooler (which Coherent will do free of charge via service contract).
Since I have been changing the water monthly it has not been a problem.

Cheers,

Brian Armstrong PhD
Light Microscopy Core
Beckman Research Institute
1450 East Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/SharedResources/LightMicroscopy/LightMicroHome.htm
 
 

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gary G. Li
Sent: Friday, October 07, 2011 9:03 AM
To: [hidden email]
Subject: Re: recurring contamination of MP cooling

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Ekaterina,

You need to add 10% OptiShield Plus (Opti Temp, Inc.
www.optishield.net) in the distilled water to prevent biological
growth in the coolant line.  OptiShield Plus protects against both
galvanic corrosion and biological buildup.

OptiShield Plus, P/N 400113, 1 Pint Size; P/N 400115, 1 Gallon Size.

Flush the coolant line more frequently until it becomes normal. Then
make coolant change monthly afterwards.

Gary G Li, PhD
Microscopy Facility Manager
NIH/NIDA at Baltimore
MD 21224, USA


On Fri, Oct 7, 2011 at 12:00 PM, Dan Focht <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Ekatrina
>
>
> I am not sure about ThermoTek brand chillers but we use a 10% alcohol and water in our recirculating chiller and have never had anything grow in it.
>
>
> Dan
>
>
>
>
>
> On Oct 7, 2011, at 11:05 AM, Ekaterina PAPUSHEVA wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear all,
>
> Need your advice regarding the contamination of our MP cooling.
>
> We were filling our ThermoTek chillers (T255P) that are connected with Cameleon II MP lasers with  distilled water, according to Coherent instructions.
> Since few days, we got dramatic contamination (some algae or bacteria growing) in the water.
>
> Even if we flash a chiller with 10 l water and refill new, we get already some visible contamination next day. One of the systems was clogged once.
>
> Are there any tips of how to get the growth out?
> We thought of adding 5% ethanol instead of water. Or building bacterial filters (like on FACS machines) in the flow circle.
>
> Does someone have experience with these two approaches, and is there something better?
>
> Looking forward to many helpful tips! Nice weekend to everyone, except those who have to flush their chillers :)
>
> Ekaterina
>
> ___
>
> Ekaterina Papusheva,
> Imaging Facility Manager
>
> Institute of Science and Technology Austria
>
> Phone  [phone];
> Mobile [phone]
> Fax    [phone]
>
> Am Campus 1
>
> A-3400 Klosterneuburg
>
> Visit our website: www.ist.ac.at<http://www.ist.ac.at>
>
>
> This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.
>
> Dan Focht
> Bioptechs, Inc.
> 3560 Beck Rd.
> Butler, PA 16002
> www.bioptechs.com
> P: [phone]
> F: [phone]
> [hidden email]
>

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[Patrick Van Oostveldt]

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*****

Including some part of the tubing by a copperpipe can solve a lot.
If more money is available a small silver pipe can also be used.

Patrick

Quoting "Dan Focht" <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Ekatrina
>
>
> I am not sure about ThermoTek brand chillers but we use a 10%  
> alcohol and water in our recirculating chiller and have never had  
> anything grow in it.
>
>
> Dan
>
>
>
>
>
> On Oct 7, 2011, at 11:05 AM, Ekaterina PAPUSHEVA wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear all,
>
> Need your advice regarding the contamination of our MP cooling.
>
> We were filling our ThermoTek chillers (T255P) that are connected  
> with Cameleon II MP lasers with  distilled water, according to  
> Coherent instructions.
> Since few days, we got dramatic contamination (some algae or  
> bacteria growing) in the water.
>
> Even if we flash a chiller with 10 l water and refill new, we get  
> already some visible contamination next day. One of the systems was  
> clogged once.
>
> Are there any tips of how to get the growth out?
> We thought of adding 5% ethanol instead of water. Or building  
> bacterial filters (like on FACS machines) in the flow circle.
>
> Does someone have experience with these two approaches, and is there  
> something better?
>
> Looking forward to many helpful tips! Nice weekend to everyone,  
> except those who have to flush their chillers :)
>
> Ekaterina
>
> ___
>
> Ekaterina Papusheva,
> Imaging Facility Manager
>
> Institute of Science and Technology Austria
>
> Phone  [phone];
> Mobile [phone]
> Fax    [phone]
>
> Am Campus 1
>
> A-3400 Klosterneuburg
>
> Visit our website: www.ist.ac.at<http://www.ist.ac.at>
>
>
> This message and any attachment (together "the Message") are  
> confidential and intended only for the individual named. If you have  
> received the Message in error you should not distribute or copy this  
> Message. Please notify the sender immediately by e-mail if you have  
> received this Message in error and delete the Message from your  
> system. Correspondence via e-mail is for information purposes. IST  
> Austria neither makes nor accepts legally binding statements unless  
> explicitly otherwise agreed to the contrary.
>
> Dan Focht
> Bioptechs, Inc.
> 3560 Beck Rd.
> Butler, PA 16002
> www.bioptechs.com
> P: [phone]
> F: [phone]
> [hidden email]
>

--
Dep. Moleculaire Biotechnologie
Coupure links 653
B 9000 GENT

tel [phone]
fax [phone]

[Guy Cox-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

Panacide is what I've used.  It came from BDH, and I imagine it's still
available.

                                        Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone [phone]     Fax [phone]
             Mobile [phone]
______________________________________________
      http://www.guycox.net
 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Ekaterina PAPUSHEVA
Sent: Saturday, 8 October 2011 2:06 AM
To: [hidden email]
Subject: recurring contamination of MP cooling

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear all,

Need your advice regarding the contamination of our MP cooling.

We were filling our ThermoTek chillers (T255P) that are connected with
Cameleon II MP lasers with  distilled water, according to Coherent
instructions.
Since few days, we got dramatic contamination (some algae or bacteria
growing) in the water.

Even if we flash a chiller with 10 l water and refill new, we get
already some visible contamination next day. One of the systems was
clogged once.

Are there any tips of how to get the growth out?
We thought of adding 5% ethanol instead of water. Or building bacterial
filters (like on FACS machines) in the flow circle.

Does someone have experience with these two approaches, and is there
something better?

Looking forward to many helpful tips! Nice weekend to everyone, except
those who have to flush their chillers :)

Ekaterina

___

Ekaterina Papusheva,
Imaging Facility Manager

Institute of Science and Technology Austria

Phone  [phone];
Mobile [phone]
Fax    [phone]

Am Campus 1

A-3400 Klosterneuburg

Visit our website: www.ist.ac.at<http://www.ist.ac.at>


This message and any attachment (together "the Message") are
confidential and intended only for the individual named. If you have
received the Message in error you should not distribute or copy this
Message. Please notify the sender immediately by e-mail if you have
received this Message in error and delete the Message from your system.
Correspondence via e-mail is for information purposes. IST Austria
neither makes nor accepts legally binding statements unless explicitly
otherwise agreed to the contrary.

-----
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[Mark Cannell]

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Do you have a reference for the antifungal activity of simple metallic silver? I've heard this repeated many times but never found a reputable source.

Cheers


On 7/10/2011, at 10:26 PM, Patrick Van Oostveldt wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Including some part of the tubing by a copperpipe can solve a lot.
> If more money is available a small silver pipe can also be used.
>
> Patrick
>
> Quoting "Dan Focht" <[hidden email]>:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Ekatrina
>>
>>
>> I am not sure about ThermoTek brand chillers but we use a 10% alcohol and water in our recirculating chiller and have never had anything grow in it.
>>
>>
>> Dan
>>
>>
>>
>>
>>
>> On Oct 7, 2011, at 11:05 AM, Ekaterina PAPUSHEVA wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear all,
>>
>> Need your advice regarding the contamination of our MP cooling.
>>
>> We were filling our ThermoTek chillers (T255P) that are connected with Cameleon II MP lasers with  distilled water, according to Coherent instructions.
>> Since few days, we got dramatic contamination (some algae or bacteria growing) in the water.
>>
>> Even if we flash a chiller with 10 l water and refill new, we get already some visible contamination next day. One of the systems was clogged once.
>>
>> Are there any tips of how to get the growth out?
>> We thought of adding 5% ethanol instead of water. Or building bacterial filters (like on FACS machines) in the flow circle.
>>
>> Does someone have experience with these two approaches, and is there something better?
>>
>> Looking forward to many helpful tips! Nice weekend to everyone, except those who have to flush their chillers :)
>>
>> Ekaterina
>>
>> ___
>>
>> Ekaterina Papusheva,
>> Imaging Facility Manager
>>
>> Institute of Science and Technology Austria
>>
>> Phone  [phone];
>> Mobile [phone]
>> Fax    [phone]
>>
>> Am Campus 1
>>
>> A-3400 Klosterneuburg
>>
>> Visit our website: www.ist.ac.at<http://www.ist.ac.at>
>>
>>
>> This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.
>>
>> Dan Focht
>> Bioptechs, Inc.
>> 3560 Beck Rd.
>> Butler, PA 16002
>> www.bioptechs.com
>> P: [phone]
>> F: [phone]
>> [hidden email]
>>
>
>
>
> --
> Dep. Moleculaire Biotechnologie
> Coupure links 653
> B 9000 GENT
>
> tel [phone]
> fax [phone]

[David Baddeley]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

you might want to run it past the manufacturer first, but we've used antifreeze to good effect in a closed circuit camera cooling loop. 

cheers,
David


________________________________
From: Patrick Van Oostveldt <[hidden email]>
To: [hidden email]
Sent: Saturday, 8 October 2011 10:26 AM
Subject: Re: recurring contamination of MP cooling

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Including some part of the tubing by a copperpipe can solve a lot.
If more money is available a small silver pipe can also be used.

Patrick

Quoting "Dan Focht" <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Ekatrina
>
>
> I am not sure about ThermoTek brand chillers but we use a 10% alcohol and water in our recirculating chiller and have never had anything grow in it.
>
>
> Dan
>
>
>
>
>
> On Oct 7, 2011, at 11:05 AM, Ekaterina PAPUSHEVA wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear all,
>
> Need your advice regarding the contamination of our MP cooling.
>
> We were filling our ThermoTek chillers (T255P) that are connected with Cameleon II MP lasers with  distilled water, according to Coherent instructions.
> Since few days, we got dramatic contamination (some algae or bacteria growing) in the water.
>
> Even if we flash a chiller with 10 l water and refill new, we get already some visible contamination next day. One of the systems was clogged once.
>
> Are there any tips of how to get the growth out?
> We thought of adding 5% ethanol instead of water. Or building bacterial filters (like on FACS machines) in the flow circle.
>
> Does someone have experience with these two approaches, and is there something better?
>
> Looking forward to many helpful tips! Nice weekend to everyone, except those who have to flush their chillers :)
>
> Ekaterina
>
> ___
>
> Ekaterina Papusheva,
> Imaging Facility Manager
>
> Institute of Science and Technology Austria
>
> Phone  [phone];
> Mobile [phone]
> Fax    [phone]
>
> Am Campus 1
>
> A-3400 Klosterneuburg
>
> Visit our website: www.ist.ac.at<http://www.ist.ac.at>
>
>
> This message and any attachment (together "the Message") are confidential and intended only for the individual named. If you have received the Message in error you should not distribute or copy this Message. Please notify the sender immediately by e-mail if you have received this Message in error and delete the Message from your system. Correspondence via e-mail is for information purposes. IST Austria neither makes nor accepts legally binding statements unless explicitly otherwise agreed to the contrary.
>
> Dan Focht
> Bioptechs, Inc.
> 3560 Beck Rd.
> Butler, PA 16002
> www.bioptechs.com
> P: [phone]
> F: [phone]
> [hidden email]
>

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[Patrick Van Oostveldt]

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Dear

Here some references at least for microbes. For furtehr details look  
at the website of our colleague Willy Verstraete.

If yoy like specific antifungal effects, this is more complex. But  
what is routinely used is treatment of the containers with ultrasound,  
which inhibits also development of algae.

References:

Sintubin, L., B. De Gusseme, et al. (2011). "The antibacterial  
activity of biogenic silver and its mode of action." Applied  
Microbiology and Biotechnology 91(1): 153-162.

Chopra, I (2007). "The increasing use of silver-based products as  
antimicrobial agents: a useful development or a cause for concern?".  
The Journal of antimicrobial chemotherapy 59 (4): 587?90.

Greetings,

Patrick Van Oostveldt
Quoting "Mark Cannell" <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Do you have a reference for the antifungal activity of simple  
> metallic silver? I've heard this repeated many times but never found  
> a reputable source.
>
> Cheers
>
>
> On 7/10/2011, at 10:26 PM, Patrick Van Oostveldt wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Including some part of the tubing by a copperpipe can solve a lot.
>> If more money is available a small silver pipe can also be used.
>>
>> Patrick
>>
>> Quoting "Dan Focht" <[hidden email]>:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Ekatrina
>>>
>>>
>>> I am not sure about ThermoTek brand chillers but we use a 10%  
>>> alcohol and water in our recirculating chiller and have never had  
>>> anything grow in it.
>>>
>>>
>>> Dan
>>>
>>>
>>>
>>>
>>>
>>> On Oct 7, 2011, at 11:05 AM, Ekaterina PAPUSHEVA wrote:
>>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Dear all,
>>>
>>> Need your advice regarding the contamination of our MP cooling.
>>>
>>> We were filling our ThermoTek chillers (T255P) that are connected  
>>> with Cameleon II MP lasers with  distilled water, according to  
>>> Coherent instructions.
>>> Since few days, we got dramatic contamination (some algae or  
>>> bacteria growing) in the water.
>>>
>>> Even if we flash a chiller with 10 l water and refill new, we get  
>>> already some visible contamination next day. One of the systems  
>>> was clogged once.
>>>
>>> Are there any tips of how to get the growth out?
>>> We thought of adding 5% ethanol instead of water. Or building  
>>> bacterial filters (like on FACS machines) in the flow circle.
>>>
>>> Does someone have experience with these two approaches, and is  
>>> there something better?
>>>
>>> Looking forward to many helpful tips! Nice weekend to everyone,  
>>> except those who have to flush their chillers :)
>>>
>>> Ekaterina
>>>
>>> ___
>>>
>>> Ekaterina Papusheva,
>>> Imaging Facility Manager
>>>
>>> Institute of Science and Technology Austria
>>>
>>> Phone  [phone];
>>> Mobile [phone]
>>> Fax    [phone]
>>>
>>> Am Campus 1
>>>
>>> A-3400 Klosterneuburg
>>>
>>> Visit our website: www.ist.ac.at<http://www.ist.ac.at>
>>>
>>>
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>>> Dan Focht
>>> Bioptechs, Inc.
>>> 3560 Beck Rd.
>>> Butler, PA 16002
>>> www.bioptechs.com
>>> P: [phone]
>>> F: [phone]
>>> [hidden email]
>>>
>>
>>
>>
>> --
>> Dep. Moleculaire Biotechnologie
>> Coupure links 653
>> B 9000 GENT
>>
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>

--
Dep. Moleculaire Biotechnologie
Coupure links 653
B 9000 GENT

tel [phone]
fax [phone]