red DNA dyes for zebrafish

[Tim Feinstein]

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Hello all,

Does anyone have a favorite 561 nm-ex DNA dye for labeling tissue such as zebrafish embryos?  I love To-Pro-3 for confocal DNA analysis; it works more reliably than exciting DAPI with a 405 nm laser and it scatters so much less in tissue.  However this time I need to use a 561 nm laser.  Many thanks,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology

[Craig Brideau]

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Well, I just saw these 'rainbow' Zebrafish on my news feed...

http://gizmodo.com/this-technicolor-mutant-zebrafish-is-synthetic-biologys-1766125296

It is actually a very interesting model for visualizing skin changes and
regeneration.

Craig

On Mon, Mar 21, 2016 at 9:25 AM, Feinstein, Timothy N <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello all,
>
> Does anyone have a favorite 561 nm-ex DNA dye for labeling tissue such as
> zebrafish embryos?  I love To-Pro-3 for confocal DNA analysis; it works
> more reliably than exciting DAPI with a 405 nm laser and it scatters so
> much less in tissue.  However this time I need to use a 561 nm laser.  Many
> thanks,
>
>
> Tim
>
> Timothy Feinstein, Ph.D.
> Research Scientist
> University of Pittsburgh Department of Developmental Biology
>

[Glen MacDonald-2]

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Hi Timothy,
Lo-Pro-3 at 2-6 uM for 20 min labels live fish (labeling neuromasts), although its  broad spectra bled into the green channel a bit.  
Never used the red Syto dyes on fixed fish.  Only in live fish, where they seemed to wander through the organellar membranes on their way to the nucleus.  LDS-751 also worked.  
Yo-Pro-1 is very bright if you can use the 488 nm line.

Regards,
Glen MacDonald
Digital Microscopy Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]

> On Mar 21, 2016, at 8:25 AM, Feinstein, Timothy N <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello all,
>
> Does anyone have a favorite 561 nm-ex DNA dye for labeling tissue such as zebrafish embryos?  I love To-Pro-3 for confocal DNA analysis; it works more reliably than exciting DAPI with a 405 nm laser and it scatters so much less in tissue.  However this time I need to use a 561 nm laser.  Many thanks,
>
>
> Tim
>
> Timothy Feinstein, Ph.D.
> Research Scientist
> University of Pittsburgh Department of Developmental Biology