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ring tirf systems

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Jeff Spector

ring tirf systems

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Greeting,
We're thinking about upgrading an old nikon scope and we are interested in
getting
a ring tirf system. Does anyone have any experience with these? Any advice
or suggestions?
All we can seem to find is the Roper iLAS2, are there other commercial
systems, or are we better off building our own?
thanks,
-Jeff

Jens

Re: ring tirf systems

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Hi Jeff,

The Ilas is nice but pretty expensive and not supported by any major microscopy software. Some vendors even want to sell you a new laser launch with it since "it only works with that launch".
Building your own is not too complicated. Just get the large galvos from thorlabs and put them into your path. I did this a while ago to rotate an oblique beam/sheet:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620878/
Another option is ASI. They make a Scanner that is not officially for ring TIRF but should work: http://www.asiimaging.com/index.php/products/light-sheet-microscopy/fiber-coupled-laser-scanner/
It can be controlled through well documented serial controls through a serial monitor. Nikon NIS has such a monitor built into its macro plugin!
We recently got one of these scanner but I hadn't have time to integrate it into our Nikon rig yet.

Best,

Jens

> Am 03.08.2016 um 18:12 schrieb Jeff Spector <[hidden email]>:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Greeting,
> We're thinking about upgrading an old nikon scope and we are interested in
> getting
> a ring tirf system. Does anyone have any experience with these? Any advice
> or suggestions?
> All we can seem to find is the Roper iLAS2, are there other commercial
> systems, or are we better off building our own?
> thanks,
> -Jeff

Edna

Re: ring tirf systems

In reply to this post by Jeff Spector

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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi, Jeff

We've been using the Roper iLas2 for the last five years.
It was mounted on a olympus IX81 body and run by metamorph. Quite stable
and easy to use. Good for experiments that need fast switching between
multiple lasers and multiple TIRF angle. Less fringe problems compare to
point TIRF systems.

(here's the publication:
http://pubs.acs.org/doi/abs/10.1021/acs.nanolett.6b00650

and here's the system specs:
http://microscopy.mechanobio.info/Home/olympustirf)

hope that helps.

Thanks.

Edna

On Thu, Aug 4, 2016 at 12:12 AM, Jeff Spector <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Greeting,
> We're thinking about upgrading an old nikon scope and we are interested in
> getting
> a ring tirf system. Does anyone have any experience with these? Any advice
> or suggestions?
> All we can seem to find is the Roper iLAS2, are there other commercial
> systems, or are we better off building our own?
> thanks,
> -Jeff
>

Deepak nair

Re: ring tirf systems

We have been using Roper iLas for last one year in the current lab with Olympus Ix 83. Before that another 4 years in my previous lab with Nikon eclipse (Daniel Choquet and Jean Baptistery Sibarita, Bordeaux). In all the cases we used it with Metamorph.

I also agree with Edna's observations. Quite stable and easy to use. We have also used it for multicolour TIRF and storm experiments.

I have no commercial interests.

Best

Deepak

On Thursday 4 August 2016, Edna <[hidden email]> wrote:

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi, Jeff

We've been using the Roper iLas2 for the last five years.
It was mounted on a olympus IX81 body and run by metamorph. Quite stable
and easy to use. Good for experiments that need fast switching between
multiple lasers and multiple TIRF angle. Less fringe problems compare to
point TIRF systems.

(here's the publication:
http://pubs.acs.org/doi/abs/10.1021/acs.nanolett.6b00650

and here's the system specs:
http://microscopy.mechanobio.info/Home/olympustirf)

hope that helps.

Thanks.

Edna

On Thu, Aug 4, 2016 at 12:12 AM, Jeff Spector <<a href="javascript:;" onclick="_e(event, 'cvml', 'jospector@gmail.com')">jospector@...> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Greeting,
> We're thinking about upgrading an old nikon scope and we are interested in
> getting
> a ring tirf system. Does anyone have any experience with these? Any advice
> or suggestions?
> All we can seem to find is the Roper iLAS2, are there other commercial
> systems, or are we better off building our own?
> thanks,
> -Jeff
>

Deepak Nair

Assistant Professor

Centre for Neuroscience

Indian Institute of Science

Bangalore 560012

Deepak nair

Re: ring tirf systems

Best

Deepak
On Tuesday 18 October 2016, Deepak Nair <[hidden email]> wrote:

Dear Jeff

We have been using Roper iLas for last one year in the current lab with Olympus Ix 83. Before that another 4 years in my previous lab with Nikon eclipse (Daniel Choquet and Jean Baptistery Sibarita, Bordeaux). In all the cases we used it with Metamorph.

I also agree with Edna's observations. Quite stable and easy to use. We have also used it for multicolour TIRF and storm experiments.

I have no commercial interests.

Best
Deepak

On Thursday 4 August 2016, Edna <<a href="javascript:_e(%7B%7D,'cvml','huxian@gmail.com');" target="_blank">huxian@...> wrote:
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi, Jeff

We've been using the Roper iLas2 for the last five years.
It was mounted on a olympus IX81 body and run by metamorph. Quite stable
and easy to use. Good for experiments that need fast switching between
multiple lasers and multiple TIRF angle. Less fringe problems compare to
point TIRF systems.

(here's the publication:
http://pubs.acs.org/doi/abs/10.1021/acs.nanolett.6b00650

and here's the system specs:
http://microscopy.mechanobio.info/Home/olympustirf)

hope that helps.

Thanks.

Edna

On Thu, Aug 4, 2016 at 12:12 AM, Jeff Spector <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Greeting,
> We're thinking about upgrading an old nikon scope and we are interested in
> getting
> a ring tirf system. Does anyone have any experience with these? Any advice
> or suggestions?
> All we can seem to find is the Roper iLAS2, are there other commercial
> systems, or are we better off building our own?
> thanks,
> -Jeff
>

--
Deepak Nair
Assistant Professor
Centre for Neuroscience
Indian Institute of Science
Bangalore 560012

Deepak Nair

Assistant Professor

Centre for Neuroscience

Indian Institute of Science

Bangalore 560012