room for two-photon microscopy

[Jurkevic, Aleksandr]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello All,

We are about to renovate the room for two-photon microscopy and I wonder what suggestions you might have about reducing light pollution that affects NDDs. As one of the measures, we are planning to paint walls and ceiling in black. What about "small" things like aluminum electrical channels, wall shelves, air vents? Have they also to be painted in dark colors or that would be an overkill? I would appreciate if you share your solutions.

Alexander


Alexander Jurkevic, PhD
Associate Director
Molecular Cytology Core
University of Missouri
120 Life Sciences Center
1201 E. Rollins St.
Columbia, MO 65211-7310

Phone:    [phone]
Fax:           [phone]

     

[Craig Brideau]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

My own experience with this very problem is that in terms of the room, it
is mainly the walls that matter.  I have shiny aluminum shelves over the
`scope, and light grey vents and the like in the ceiling, and it doesn't
seem to make much difference.  One key detail is that you want the walls
painted in matte black, not glossy.  The contractors might mix this up so
be very specific.  Now our biggest issue is the clothing worn by the
researchers; they were wearing white lab coats which was scattering light
from the computer monitors back towards the instruments.  I have dark navy
blue lab coats on hand that solve that problem, or the researchers turn the
monitor off/away for long runs.  If what you are doing is very picky (low
signal, lots of gain) a little bit of blackout cloth suspended in front of
the stage can help as well.  We've (carefully) draped a bit over the
eyepieces on occasion, letting it hang down in front of the stage as a
quick but effective light shield.

Craig



On Mon, Dec 9, 2013 at 5:40 PM, Jurkevic, Aleksandr
<[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
>
> We are about to renovate the room for two-photon microscopy and I wonder
> what suggestions you might have about reducing light pollution that affects
> NDDs. As one of the measures, we are planning to paint walls and ceiling in
> black. What about "small" things like aluminum electrical channels, wall
> shelves, air vents? Have they also to be painted in dark colors or that
> would be an overkill? I would appreciate if you share your solutions.
>
> Alexander
>
>
> Alexander Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone:    [phone]
> Fax:           [phone]
>
>
>

[Mark Cannell-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

There is no need to go so far as to paint walls black if you eliminate light sources. A solid Faraday cage around the scope with a black curtain and interior  is just as good and fixes the monitor light pollution problem. The room lighting should be easily dimmable from the scope/workstation.

HTH

Mark

On 10/12/2013, at 12:40 am, Jurkevic, Aleksandr <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
>
> We are about to renovate the room for two-photon microscopy and I wonder what suggestions you might have about reducing light pollution that affects NDDs. As one of the measures, we are planning to paint walls and ceiling in black. What about "small" things like aluminum electrical channels, wall shelves, air vents? Have they also to be painted in dark colors or that would be an overkill? I would appreciate if you share your solutions.
>
> Alexander
>
>
> Alexander Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone:    [phone]
> Fax:           [phone]
>
>

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]

[Pascal Weber]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Alexander,

I install many two microscopes. Infact i works on plants and mice.I built a blac
box around my upright microscope.It's very important to eliminate any light
pollution.
So i have many pictures of my rooms. My mail
[hidden email]

[Craig Brideau]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I agree its best if you can just box in the microscope itself: Then you
don't have to worry about the room so much.  In our case, however, we work
with a lot of live samples that require a lot of checking on, and plumbing
and heaters that don't work well with an enclosure, which is why we
darkened the entire room.  That way you have relatively free access to poke
and prod your sample.  If you don't need easy access, or don't mind
constantly opening the enclosure to check on your sample, the box method
works very well and is certainly simpler.  If you are building a room from
scratch though and have the option, I still recommend painting the walls a
dark colour as the cost difference will be small compared to the
traditional white or beige paint and will always give an advantage.

Craig


On Mon, Dec 9, 2013 at 6:44 PM, Mark Cannell <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> There is no need to go so far as to paint walls black if you eliminate
> light sources. A solid Faraday cage around the scope with a black curtain
> and interior  is just as good and fixes the monitor light pollution
> problem. The room lighting should be easily dimmable from the
> scope/workstation.
>
> HTH
>
> Mark
>
> On 10/12/2013, at 12:40 am, Jurkevic, Aleksandr <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hello All,
> >
> > We are about to renovate the room for two-photon microscopy and I wonder
> what suggestions you might have about reducing light pollution that affects
> NDDs. As one of the measures, we are planning to paint walls and ceiling in
> black. What about "small" things like aluminum electrical channels, wall
> shelves, air vents? Have they also to be painted in dark colors or that
> would be an overkill? I would appreciate if you share your solutions.
> >
> > Alexander
> >
> >
> > Alexander Jurkevic, PhD
> > Associate Director
> > Molecular Cytology Core
> > University of Missouri
> > 120 Life Sciences Center
> > 1201 E. Rollins St.
> > Columbia, MO 65211-7310
> >
> > Phone:    [phone]
> > Fax:           [phone]
> >
> >
>
> Mark  B. Cannell Ph.D. FRSNZ
> Professor of Cardiac Cell Biology
> School of Physiology &  Pharmacology
> Medical Sciences Building
> University of Bristol
> Bristol
> BS8 1TD UK
>
> [hidden email]
>

[Mark Cannell-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Ok, but thinking outside the box, the key is to eliminate/control sources. As long as the NDD can’t see the sources you don’t need a black room. I would prefer a big box around the scope -it could be big enough to let you fiddle but still block the monitor which is a major source of light ion the room. Even a single shield between the monitor and scope will work and then use mat dark green/blue walls and use a red filter over the monitor giving a much more pleasant work environment.

HTH

Cheers Mark





On 10/12/2013, at 2:01 am, Craig Brideau <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I agree its best if you can just box in the microscope itself: Then you
> don't have to worry about the room so much.  In our case, however, we work
> with a lot of live samples that require a lot of checking on, and plumbing
> and heaters that don't work well with an enclosure, which is why we
> darkened the entire room.  That way you have relatively free access to poke
> and prod your sample.  If you don't need easy access, or don't mind
> constantly opening the enclosure to check on your sample, the box method
> works very well and is certainly simpler.  If you are building a room from
> scratch though and have the option, I still recommend painting the walls a
> dark colour as the cost difference will be small compared to the
> traditional white or beige paint and will always give an advantage.
>
> Craig
>
>
> On Mon, Dec 9, 2013 at 6:44 PM, Mark Cannell <[hidden email]>wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> There is no need to go so far as to paint walls black if you eliminate
>> light sources. A solid Faraday cage around the scope with a black curtain
>> and interior  is just as good and fixes the monitor light pollution
>> problem. The room lighting should be easily dimmable from the
>> scope/workstation.
>>
>> HTH
>>
>> Mark
>>
>> On 10/12/2013, at 12:40 am, Jurkevic, Aleksandr <[hidden email]>
>> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Hello All,
>>>
>>> We are about to renovate the room for two-photon microscopy and I wonder
>> what suggestions you might have about reducing light pollution that affects
>> NDDs. As one of the measures, we are planning to paint walls and ceiling in
>> black. What about "small" things like aluminum electrical channels, wall
>> shelves, air vents? Have they also to be painted in dark colors or that
>> would be an overkill? I would appreciate if you share your solutions.
>>>
>>> Alexander
>>>
>>>
>>> Alexander Jurkevic, PhD
>>> Associate Director
>>> Molecular Cytology Core
>>> University of Missouri
>>> 120 Life Sciences Center
>>> 1201 E. Rollins St.
>>> Columbia, MO 65211-7310
>>>
>>> Phone:    [phone]
>>> Fax:           [phone]
>>>
>>>
>>
>> Mark  B. Cannell Ph.D. FRSNZ
>> Professor of Cardiac Cell Biology
>> School of Physiology &  Pharmacology
>> Medical Sciences Building
>> University of Bristol
>> Bristol
>> BS8 1TD UK
>>
>> [hidden email]
>>

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]

[Pascal Weber]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I work primarily on the mouse. I installed my microscope with a dark part and a
bright part (natural) to my surgeries. I had to create a black heated around my
upright microscope box. I have all the references to build oneself. I also modify
the plate to accept the sample. The walls were painted dark blue mat. But
anyway your laser should never leave the safety casing. Know that dark walls
absolutely no protection from parasites and dangerous reflections pulsed
infrared lasers. My Systems: Zeiss Maitai.
I'm building  a 2P + OPO. Coherent.

[Wilson, Sean (LLU)]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

We used the K.I.S.S. principle on our ziess 710 NLO. Based on empirical evidence, we ended up draping blackout cloth across the top of the scope to keep out ambient light from the monitor, ect. & turned off external light sources (i.e. EXFO).

_____________________________________________
Sean M. Wilson, Ph.D.
Assistant Professor, Center for Perinatal Biology
Technical Director, Advanced Imaging and Microscopy Core Facility
Loma Linda University School of Medicine Loma Linda, CA 92350
[hidden email]
909-558-4325 (Main Office)
x45209 (Note new campus extension)
x42183 (RW 1561 laboratory)
909-558-4029 (Fax)
909-647-7021 (Cell)

http://microscopy.llu.edu
Check out our video on the homepage!
http://www.llu.edu/medicine/basic-sciences/faculty/pharmacology/wilson-sean.page?

CONFIDENTIALITY NOTICE: This e-mail and any attachments are confidential and may also be privileged. If you are not the named recipient, please notify the sender immediately and delete the contents of this message without disclosing the contents to anyone, using them for any purpose, or storing or copying the information on any medium.

________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Pascal Weber [[hidden email]]
Sent: Monday, December 09, 2013 6:27 PM
To: [hidden email]
Subject: Re: room for two-photon microscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I work primarily on the mouse. I installed my microscope with a dark part and a
bright part (natural) to my surgeries. I had to create a black heated around my
upright microscope box. I have all the references to build oneself. I also modify
the plate to accept the sample. The walls were painted dark blue mat. But
anyway your laser should never leave the safety casing. Know that dark walls
absolutely no protection from parasites and dangerous reflections pulsed
infrared lasers. My Systems: Zeiss Maitai.
I'm building  a 2P + OPO. Coherent.

[Sylvie Le Guyader]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi there

We have built low table-type things to raise the equipment that is normally on the floor like the 2P laser cooler and power supply. This way the fans do not suck the dust in and it is easier to keep the room clean.

We have also built large cheap rectangular boxes that are placed just above the equipment that produces heat (2P cooler and power supply, argon laser power supply). These boxes are about 30 cm above the equipment and are connected to the normal ventilation system of the building. They channel the heat out very efficiently as there is a weakish but constant air suction. You can avoid costly ventilation work that way. On top of that we have an air con unit set to 23deg Celsius so it is quite comfortable to work in the room.

The best way is of course to have a heat and noise producing equipment in an adjacent room and a hole in the wall for the cables.

We have a full size incubator on which we can add black panels when we use the NDDs or the GaAsP.

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Live Cell Imaging Unit
Dept of Biosciences and Nutrition
Karolinska Institutet
Hälsovägen 7
Novum, G lift, floor 6
14157 Huddinge
Sweden
office: [phone]
LCI room 1: [phone]
LCI room 2: [phone]
mobile: [phone]

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of Jurkevic,
> Aleksandr
> Sent: 10 December 2013 01:40
> To: [hidden email]
> Subject: room for two-photon microscopy
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
>
> We are about to renovate the room for two-photon microscopy and I wonder what
> suggestions you might have about reducing light pollution that affects NDDs. As
> one of the measures, we are planning to paint walls and ceiling in black. What
> about "small" things like aluminum electrical channels, wall shelves, air vents? Have
> they also to be painted in dark colors or that would be an overkill? I would
> appreciate if you share your solutions.
>
> Alexander
>
>
> Alexander Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone:    [phone]
> Fax:           [phone]
>
>

[Watkins, Simon C]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Apart from the endless idiot lights that need to be taped over the monitor
is the biggest issue.  The best solution in our hands has been to use
security film (3M) which makes the monitor a little dimmer straight on but
effectively removes all light at an angle and removes the need for draping
curtains etc.  On our second system we have built the proverbial black
plexiglass box, which provides heat and allows room lightŠ it is certainly
the best solution all round, I can send pics and design details if anyone
wants them (actually you can see it if you look under Nikon multi photon
in the AAA section of our resource page at www.cbi.pitt.edu).
S.

Simon Watkins Ph.D

Professor and Vice Chair Cell Biology
Professor Immunology
Director Center for Biologic Imaging
University of Pittsburgh
Bsts 225 3550 terrace st
Pittsburgh PA 15261
Www.cbi.pitt.edu <http://Www.cbi.pitt.edu/>
412-352-2277






On 12/9/13, 7:40 PM, "Jurkevic, Aleksandr" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Hello All,
>
>We are about to renovate the room for two-photon microscopy and I wonder
>what suggestions you might have about reducing light pollution that
>affects NDDs. As one of the measures, we are planning to paint walls and
>ceiling in black. What about "small" things like aluminum electrical
>channels, wall shelves, air vents? Have they also to be painted in dark
>colors or that would be an overkill? I would appreciate if you share your
>solutions.
>
>Alexander
>
>
>Alexander Jurkevic, PhD
>Associate Director
>Molecular Cytology Core
>University of Missouri
>120 Life Sciences Center
>1201 E. Rollins St.
>Columbia, MO 65211-7310
>
>Phone:    [phone]
>Fax:           [phone]
>
>    

[Tobias Rose]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

We usually use a dark cage as well. However, since we use monitors for visual stimulation of living animals we also synchronize the LED backlight of the screens to the turnaround phase of our resonant scanners. This way, the screens (both for stimulation and in some case also all remaining screens in the room) are only bright during the ~30% of scanning time where we reject the acquired data anyways. Works like a charm.

Tobias
(Leinweber et al. 2013, JOVE, in press)

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jurkevic, Aleksandr
Sent: Tuesday, December 10, 2013 01:40
To: [hidden email]
Subject: room for two-photon microscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello All,

We are about to renovate the room for two-photon microscopy and I wonder what suggestions you might have about reducing light pollution that affects NDDs. As one of the measures, we are planning to paint walls and ceiling in black. What about "small" things like aluminum electrical channels, wall shelves, air vents? Have they also to be painted in dark colors or that would be an overkill? I would appreciate if you share your solutions.

Alexander


Alexander Jurkevic, PhD
Associate Director
Molecular Cytology Core
University of Missouri
120 Life Sciences Center
1201 E. Rollins St.
Columbia, MO 65211-7310

Phone:    [phone]
Fax:           [phone]

     

[Arvydas Matiukas]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello List,
 
We are going to move to new  research building with all
current microscopy equipment. Few new microscopes (
confocal, 2-photon and STED-superresolution)
will be installed as well.  I ordered all the walls painted
matte black. I hope it makes a lot of difference . You
already can notice that when making a photo.
We installed red overhead light as an selectable option.
 
Taking the occasion of the thread I would like to ask
about other important issues to follow up before we
move. Manufacturers of microscopes suggest to check
the room for vibration, conditioning airflow, dust-free,
clean power. Any feedback/advise is highly appreciated.
 
Best regards,
Arvydas
 
 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
Department of Neurosci& Physiology
SUNY Upstate Medical University
766 Irving Ave., WH 3167
Syracuse, NY 13210
tel.: [phone]
fax: [phone]
email: [hidden email]
>>> "Jurkevic, Aleksandr" <[hidden email]> 12/9/2013 7:40 PM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello All,

We are about to renovate the room for two-photon microscopy and I wonder what suggestions you might have about reducing light pollution that affects NDDs. As one of the measures, we are planning to paint walls and ceiling in black. What about "small" things like aluminum electrical channels, wall shelves, air vents? Have they also to be painted in dark colors or that would be an overkill? I would appreciate if you share your solutions.

Alexander


Alexander Jurkevic, PhD
Associate Director
Molecular Cytology Core
University of Missouri
120 Life Sciences Center
1201 E. Rollins St.
Columbia, MO 65211-7310

Phone:    [phone]
Fax:           [phone]

     

[Craig Brideau]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

You will need cooling to handle the heat from the lamps and lasers, as well
as the people occupying the room.  You will want filtered air to keep dust
to a minimum.  Temperature should be stable to +/-1 degree C, which is
often one of the hardest criteria to meet.  Humidity should be kept
reasonable, and provision should be made for dehumidification if it is a
damp environment.  Regarding vibration, is the room on an upper floor?
 Basement is ideal, but if you are on an upper floor you will want good
passively-damped tables that are fairly thick.  Also make sure you can get
the optical tables into place without having to knock a hole in the wall,
so check door widths, elevator capacities, etc.  Make sure light switches
for the room are near where the operator(s) will sit.  Make sure sample
prep areas are nearby and appropriately equipped (sinks, incubators,
whatever you need).  Make sure you have mounts for any gas bottles and that
they meet the safety standards required in your area.

That's what I've got off the top of my head, I'm sure others will have much
more to add!

Craig


On Wed, Dec 11, 2013 at 3:02 PM, Arvydas Matiukas <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello List,
>
> We are going to move to new  research building with all
> current microscopy equipment. Few new microscopes (
> confocal, 2-photon and STED-superresolution)
> will be installed as well.  I ordered all the walls painted
> matte black. I hope it makes a lot of difference . You
> already can notice that when making a photo.
> We installed red overhead light as an selectable option.
>
> Taking the occasion of the thread I would like to ask
> about other important issues to follow up before we
> move. Manufacturers of microscopes suggest to check
> the room for vibration, conditioning airflow, dust-free,
> clean power. Any feedback/advise is highly appreciated.
>
> Best regards,
> Arvydas
>
>
>
> Arvydas Matiukas, Ph.D.
> Director of Confocal&Two-Photon Core
> Department of Neurosci& Physiology
> SUNY Upstate Medical University
> 766 Irving Ave., WH 3167
> Syracuse, NY 13210
> tel.: [phone]
> fax: [phone]
> email: [hidden email]
> >>> "Jurkevic, Aleksandr" <[hidden email]> 12/9/2013 7:40 PM >>>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
>
> We are about to renovate the room for two-photon microscopy and I wonder
> what suggestions you might have about reducing light pollution that affects
> NDDs. As one of the measures, we are planning to paint walls and ceiling in
> black. What about "small" things like aluminum electrical channels, wall
> shelves, air vents? Have they also to be painted in dark colors or that
> would be an overkill? I would appreciate if you share your solutions.
>
> Alexander
>
>
> Alexander Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone:    [phone]
> Fax:           [phone]
>
>
>

[mcammer]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

If you are using Isoflurane or other gas based anesthesia, make sure the room is well ventilated even when you have a charcoal filter in the microscope's environmental chamber.

===========================================================================
Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center
Cell:  914-309-3270   Lab: 212-263-3208
http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Arvydas Matiukas
Sent: Wednesday, December 11, 2013 5:03 PM
To: [hidden email]
Subject: Re: room for two-photon microscopy: checking environment

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello List,
 
We are going to move to new  research building with all current microscopy equipment. Few new microscopes ( confocal, 2-photon and STED-superresolution) will be installed as well.  I ordered all the walls painted matte black. I hope it makes a lot of difference . You already can notice that when making a photo.
We installed red overhead light as an selectable option.
 
Taking the occasion of the thread I would like to ask about other important issues to follow up before we move. Manufacturers of microscopes suggest to check the room for vibration, conditioning airflow, dust-free, clean power. Any feedback/advise is highly appreciated.
 
Best regards,
Arvydas
 
 
 
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
Department of Neurosci& Physiology
SUNY Upstate Medical University
766 Irving Ave., WH 3167
Syracuse, NY 13210
tel.: [phone]
fax: [phone]
email: [hidden email]
>>> "Jurkevic, Aleksandr" <[hidden email]> 12/9/2013 7:40 PM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hello All,

We are about to renovate the room for two-photon microscopy and I wonder what suggestions you might have about reducing light pollution that affects NDDs. As one of the measures, we are planning to paint walls and ceiling in black. What about "small" things like aluminum electrical channels, wall shelves, air vents? Have they also to be painted in dark colors or that would be an overkill? I would appreciate if you share your solutions.

Alexander


Alexander Jurkevic, PhD
Associate Director
Molecular Cytology Core
University of Missouri
120 Life Sciences Center
1201 E. Rollins St.
Columbia, MO 65211-7310

Phone:    [phone]
Fax:           [phone]

     

------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
=================================

[Craig Brideau]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

To add to the ventilation comment, we had a flexible tube installed near
the microscope connected to a filtered exhaust.  When we are using
isoflurane we can place the open end of the tube near the stage.  It has a
mild draw on it, not strong enough to disrupt the environment, but strong
enough to pull the gas away from the user.

Craig Brideau


On Thu, Dec 19, 2013 at 10:48 AM, Cammer, Michael <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> If you are using Isoflurane or other gas based anesthesia, make sure the
> room is well ventilated even when you have a charcoal filter in the
> microscope's environmental chamber.
>
> ===========================================================================
> Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU
> Langone Medical Center
> Cell:  914-309-3270   Lab: 212-263-3208
> http://ocs.med.nyu.edu/microscopy &
> http://www.med.nyu.edu/skirball-lab/dustinlab/
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Arvydas Matiukas
> Sent: Wednesday, December 11, 2013 5:03 PM
> To: [hidden email]
> Subject: Re: room for two-photon microscopy: checking environment
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello List,
>
> We are going to move to new  research building with all current microscopy
> equipment. Few new microscopes ( confocal, 2-photon and
> STED-superresolution) will be installed as well.  I ordered all the walls
> painted matte black. I hope it makes a lot of difference . You already can
> notice that when making a photo.
> We installed red overhead light as an selectable option.
>
> Taking the occasion of the thread I would like to ask about other
> important issues to follow up before we move. Manufacturers of microscopes
> suggest to check the room for vibration, conditioning airflow, dust-free,
> clean power. Any feedback/advise is highly appreciated.
>
> Best regards,
> Arvydas
>
>
>
> Arvydas Matiukas, Ph.D.
> Director of Confocal&Two-Photon Core
> Department of Neurosci& Physiology
> SUNY Upstate Medical University
> 766 Irving Ave., WH 3167
> Syracuse, NY 13210
> tel.: [phone]
> fax: [phone]
> email: [hidden email]
> >>> "Jurkevic, Aleksandr" <[hidden email]> 12/9/2013 7:40 PM >>>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello All,
>
> We are about to renovate the room for two-photon microscopy and I wonder
> what suggestions you might have about reducing light pollution that affects
> NDDs. As one of the measures, we are planning to paint walls and ceiling in
> black. What about "small" things like aluminum electrical channels, wall
> shelves, air vents? Have they also to be painted in dark colors or that
> would be an overkill? I would appreciate if you share your solutions.
>
> Alexander
>
>
> Alexander Jurkevic, PhD
> Associate Director
> Molecular Cytology Core
> University of Missouri
> 120 Life Sciences Center
> 1201 E. Rollins St.
> Columbia, MO 65211-7310
>
> Phone:    [phone]
> Fax:           [phone]
>
>
>
> ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain information that is proprietary,
> confidential, and exempt from disclosure under applicable law. Any
> unauthorized review, use, disclosure, or distribution is prohibited. If you
> have received this email in error please notify the sender by return email
> and delete the original message. Please note, the recipient should check
> this email and any attachments for the presence of viruses. The
> organization accepts no liability for any damage caused by any virus
> transmitted by this email.
> =================================
>