screening 96well plates with Zeiss Zen2011

[Martin Schattat]

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Hi List,

at first thanks for the suggestions I got from my last questions. It is amazing how lively this forum is. I have now a different question. I am going to do a screen of mutant plants, of which I am going to pick leafs and place them in 96 well plates. These I plant to image with an inverted microscope. I had a Zeiss sales man here and he told me that this is no problem. I have not done work like this with Zen and would like to know if some one has experience with this. I am planing on having a wide field system for this purpose. I would be interested if the Software is hard to teach, where to look and if it is able to handle the amount of data produced?

Thanks in advance

Martin


-------------------------------------------------------------------
Dr. Martin Schattat
Martin-Luther-Universität Halle-Wittenberg
Institutsbereich Pflanzenphysiologie
Weinbergweg 10, 06120 Halle (Saale), Germany

Tel.:     [phone]
Email: [hidden email]

[Mei, Erwen (GE Healthcare)]

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Hi, Martin,
 
Since You plan to do the screening, I would like to recommend you to look at some fully automated microscopic platform, such as IN Cell Analyzer 2200 by GE Healthcare for your experiment. The platform comes with SW for analyzing your data. This kind of platform is ideal for high throughput screening.


Erwen Mei


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Schattat
Sent: Friday, January 18, 2013 7:42 AM
To: [hidden email]
Subject: screening 96well plates with Zeiss Zen2011

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To join, leave or search the confocal microscopy listserv, go to:
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Hi List,

at first thanks for the suggestions I got from my last questions. It is amazing how lively this forum is. I have now a different question. I am going to do a screen of mutant plants, of which I am going to pick leafs and place them in 96 well plates. These I plant to image with an inverted microscope. I had a Zeiss sales man here and he told me that this is no problem. I have not done work like this with Zen and would like to know if some one has experience with this. I am planing on having a wide field system for this purpose. I would be interested if the Software is hard to teach, where to look and if it is able to handle the amount of data produced?

Thanks in advance

Martin


-------------------------------------------------------------------
Dr. Martin Schattat
Martin-Luther-Universität Halle-Wittenberg Institutsbereich Pflanzenphysiologie Weinbergweg 10, 06120 Halle (Saale), Germany

Tel.:     [phone]
Email: [hidden email]

[Justin Price]

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Dear Martin,

Zen 2011 is able to perform the 96-well screening by using the positions
module in the software.  You can either mark off individual locations
manually and save them into a list or you could see if there is a sample
carrier option that will allow you to make a template for scanning.

The analysis software that can be programmed/taught to process images based
on your specifications but a bit of background knowledge in basic image
processing will help when designing your algorithms.  These algorithms can
be saved and used by various users with minimal amount of training.

The overall layout of Zen is very friendly and I have found that training
users that have a little bit of microscopy experience is easy because the
GUI seems to follow a natural left to right, top to bottom sequence of
setting up experiments.  Users that don't have any microscopy experience
can be introduced to saved experiments that will handle a vast majority of
the acquisition parameters so it could be as easy as point and click to
acquire data.

I believe the newest version was only written for 64-bit systems so the
amount of data that the software can handle is limited by the amount of RAM
and/or total storage space that you machine has access to.


Hope this helps,
Justin Price


On Fri, Jan 18, 2013 at 7:41 AM, Martin Schattat <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi List,
>
> at first thanks for the suggestions I got from my last questions. It is
> amazing how lively this forum is. I have now a different question. I am
> going to do a screen of mutant plants, of which I am going to pick leafs
> and place them in 96 well plates. These I plant to image with an inverted
> microscope. I had a Zeiss sales man here and he told me that this is no
> problem. I have not done work like this with Zen and would like to know if
> some one has experience with this. I am planing on having a wide field
> system for this purpose. I would be interested if the Software is hard to
> teach, where to look and if it is able to handle the amount of data
> produced?
>
> Thanks in advance
>
> Martin
>
>
> -------------------------------------------------------------------
> Dr. Martin Schattat
> Martin-Luther-Universität Halle-Wittenberg
> Institutsbereich Pflanzenphysiologie
> Weinbergweg 10, 06120 Halle (Saale), Germany
>
> Tel.:     [phone]
> Email: [hidden email]
>

--
Justin P.