special slide glass?

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Oky Oky
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special slide glass?

Search the CONFOCAL archive at
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Hello,

I am looking for a slide glass that will be used for an inverted
microscope. My issue is that my sample cannot be mounted between a
slide glass and cover slip. I should apply a treatment to the sample
while I observe the sample. (My sample is wood cell wall.)

Does anyone have a good idea? Thank you very much.

--
Ohkyung Kwon, Ph D

http://www.linkedin.com/in/ohkyung
http://www.wpskorea.org
Benoit Labarthe Benoit Labarthe
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Re: special slide glass?

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi,

I'm using glass bottom Petri Dishes from Mattek with good results, other compagnies are offering such petri dishes. (No commercial interest)

cheers

benoit



On Tue, Mar 18, 2008 at 4:39 PM, Oky <[hidden email]> wrote:
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello,

I am looking for a slide glass that will be used for an inverted
microscope. My issue is that my sample cannot be mounted between a
slide glass and cover slip. I should apply a treatment to the sample
while I observe the sample. (My sample is wood cell wall.)

Does anyone have a good idea? Thank you very much.

--
Ohkyung Kwon, Ph D

http://www.linkedin.com/in/ohkyung
http://www.wpskorea.org



--
Benoît Labarthe, PharmD
Institut de Cardiologie de Montréal
Montreal Heart Institute
Centre de Recherche, Laboratoire du Dr.Théroux
5000 Bélanger Est
Montréal Québec Canada H1T 1C8
Tel (514)-376 3330 ext 3017
Fax (514)- 376 1076
Bill Miller-3 Bill Miller-3
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Re: special slide glass?

In reply to this post by Oky
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

cut a hole in a regular slide and epoxy a cover slip over the hole..


At 05:39 PM 3/18/2008 -0400, you wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello,
>
>I am looking for a slide glass that will be used for an inverted
>microscope. My issue is that my sample cannot be mounted between a
>slide glass and cover slip. I should apply a treatment to the sample
>while I observe the sample. (My sample is wood cell wall.)
>
>Does anyone have a good idea? Thank you very much.
>
>--
>Ohkyung Kwon, Ph D
>
>http://www.linkedin.com/in/ohkyung
>http://www.wpskorea.org
Louis Kerr Louis Kerr
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Re: special slide glass?

In reply to this post by Oky
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Two options that come to mind are the MatTek type petri dishes
http://www.glass-bottom-dishes.com/free-sample.html or have your machine
shop make slide shaped blanks out of 1mm thick aluminum or plastic. Then
you can cut or drill out any shape. We routinely cut a 15x15mm square
notch at the middle of the slide opening onto one side of the slide. Now
you can tape (or glue) a 22x22mm cover slip over the open area and use
it on an inverted microscope with the cover slip below the sample and
open above. You can tape another cover slip on the other side of the
slide to have a coverslip-sample-coverslip sandwich with 1mm between
coverslips. All this is predicated on the assumption that the slide does
not need to be glass.

Louie

Oky wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello,
>
> I am looking for a slide glass that will be used for an inverted
> microscope. My issue is that my sample cannot be mounted between a
> slide glass and cover slip. I should apply a treatment to the sample
> while I observe the sample. (My sample is wood cell wall.)
>
> Does anyone have a good idea? Thank you very much.
>

--
Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA  02543
508-289-7273
508-540-6902 (FAX)
508-292-0289 (Cell phone)

VISIT OUR WEB SITES:
http://www.mbl.edu/
http://www.courses.mbl.edu/
Stanislav Vitha Stanislav Vitha
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Re: special slide glass?

In reply to this post by Oky
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

besides the Mattek dishes mentioned before, you can make your own (see
http://www.science.mcmaster.ca/biochem/faculty/truant/protocols.htm).

I had good luck with using a 1/4" or 1/2" thick piece of plexiglass, with a 20mm
hole drilled in the center, with coverslip glued over the hole using GE Silicone
II sealant (most hardware stores carry that). The sealant cures in about a
day, but remains flexible, so when the coverslip breaks, it is easy to scrape it
off witjh a razor blade and glue a new one on.

Stan Vitha

On Tue, 18 Mar 2008 17:39:30 -0400, Oky <[hidden email]>
wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello,
>
>I am looking for a slide glass that will be used for an inverted
>microscope. My issue is that my sample cannot be mounted between a
>slide glass and cover slip. I should apply a treatment to the sample
>while I observe the sample. (My sample is wood cell wall.)
>
>Does anyone have a good idea? Thank you very much.
>
>--
>Ohkyung Kwon, Ph D
>
>http://www.linkedin.com/in/ohkyung
>http://www.wpskorea.org
Oky Oky
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Re: special slide glass?

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Thank you for your suggestions. Have a nice weekend!

--
Ohkyung Kwon, Ph D

http://www.linkedin.com/in/ohkyung
http://www.wpskorea.org