stagetop incubators
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Greetings, I am looking in to purchasing a stage top incubator for our spinning disk system so we can do live cell imaging. I've looked a lot at the Tokai Hit setup but have also heard good things about stuff from OKO lab. Do you microscopy experts have any suggestions/advice, or opinions about what system we should use? thanks! -Jeff |
 
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Prakash, Y. S. (Y S), M.D., Ph.D. |
Re: stagetop incubators
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Jeff We have had the OKO Lab system with the capability for continuous as well as intermittent changes in both O2 and CO2. The system sits on a Nikon inverted scope and is controlled through Nikon Elements which makes it really nice to integrate with the imaging (XYZ stage controller). Love it. Haven't found too many things to hate yet. On 5/14/14 8:15 PM, "Jeff Spector" <[hidden email]> wrote: >***** >To join, leave or search the confocal microscopy listserv, go to: >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >Post images on http://www.imgur.com and include the link in your posting. >***** > >Greetings, > I am looking in to purchasing a stage top incubator for our spinning >disk system so we can do live cell imaging. I've looked a lot at the Tokai >Hit setup but have also heard good things about stuff from OKO lab. Do >you >microscopy experts have any suggestions/advice, or opinions about what >system we should use? > > thanks! > -Jeff |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Jeff, I'm sure the OKO lab system is good, but you might want to check out Kairos Instruments, LLC systems before you decide. www.kairosinstruments.com We're located in Pittsburgh, PA. Al Bahnson, Partner Kairos Instruments, LLC 412-735-9983 On Wed, May 14, 2014 at 9:25 PM, Prakash, Y. S. (Y S), M.D., Ph.D. < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Jeff > We have had the OKO Lab system with the capability for continuous as well > as intermittent changes in both O2 and CO2. The system sits on a Nikon > inverted scope and is controlled through Nikon Elements which makes it > really nice to integrate with the imaging (XYZ stage controller). Love it. > Haven't found too many things to hate yet. > > > On 5/14/14 8:15 PM, "Jeff Spector" <[hidden email]> wrote: > > >***** > >To join, leave or search the confocal microscopy listserv, go to: > >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >Post images on http://www.imgur.com and include the link in your posting. > >***** > > > >Greetings, > > I am looking in to purchasing a stage top incubator for our spinning > >disk system so we can do live cell imaging. I've looked a lot at the Tokai > >Hit setup but have also heard good things about stuff from OKO lab. Do > >you > >microscopy experts have any suggestions/advice, or opinions about what > >system we should use? > > > > thanks! > > -Jeff > |
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In reply to this post by Jeff Spector
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Jeff Setting up a live-cell micro-environment for time lapse imaging requires attention to many technical details. If you go to the Bioptechs site www.bioptechs.com you will find a link on the home page for a profile questionnaire. If you fill this out and return it to us so that we understand your entire system and specimens requirements, we can recommend a comprehensive system. We can also send you a demo system to try for a week so you will be sure you like it. You will find from the questionnaire that we take a very detailed approach to meeting your needs in a cost effective way using the latest technologies. You will also find a link for published references as well as the numerous approaches to live cell imaging for upright, inverted and stereo microscopes compatible with all the major manufacturers. Dan On 5/14/2014 9:15 PM, Jeff Spector wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Greetings, > I am looking in to purchasing a stage top incubator for our spinning > disk system so we can do live cell imaging. I've looked a lot at the Tokai > Hit setup but have also heard good things about stuff from OKO lab. Do you > microscopy experts have any suggestions/advice, or opinions about what > system we should use? > > thanks! > -Jeff > > -- Dan Focht Bioptechs Inc. V: (724)282-7145 www.bioptechs.com |
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In reply to this post by Jeff Spector
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Jeff, We looked at several options including the ones you mentioned, but decided on In Vivo Scientific. They are significantly cheaper than Tokai and Oko Labs and are very user friendly. In addition, for our specific needs, they even did the modification without any costs. I would strongly recommend them. Contact me offline for any other questions. Regards, -Prabhakar On 5/14/2014 8:15 PM, Jeff Spector wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Greetings, > I am looking in to purchasing a stage top incubator for our spinning > disk system so we can do live cell imaging. I've looked a lot at the Tokai > Hit setup but have also heard good things about stuff from OKO lab. Do you > microscopy experts have any suggestions/advice, or opinions about what > system we should use? > > thanks! > -Jeff > -- B. Prabhakar Pandian, PhD Manager, Cellular and Biomolecular Engineering Director, Bioengineering Lab Core CFD Research Corporation 701 McMillan Way, Suite D Huntsville, AL 35806 Web:www.cfdrc.com ------------------------------------------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE The information contained in this communication and its attachments is intended only for the use of the individual to whom it is addressed and may contain information that is legally privileged, confidential, or exempt from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, or reliance on this communication is strictly prohibited. If you have received this communication in error, please notify [hidden email] (256-726-4800) and delete the communication without retaining any copies. |
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In reply to this post by Jeff Spector
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Another worth looking at is Bioptechs.com. Kind Regards, Another Jeff From: Jeff Spector Sent: Wednesday, May 14, 2014 9:15 PM To: [hidden email] Subject: stagetop incubators ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Greetings, I am looking in to purchasing a stage top incubator for our spinning disk system so we can do live cell imaging. I've looked a lot at the Tokai Hit setup but have also heard good things about stuff from OKO lab. Do you microscopy experts have any suggestions/advice, or opinions about what system we should use? thanks! -Jeff |
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In reply to this post by Jeff Spector
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Jeff, We have several types of incubators in our facility: PeCon (comes with Zeiss systems), LIS (comes with Leica systems, at least in CH), OkoLab. All of these systems are cage incubators and offer stable and reliable temp. control. PeCon and OkoLab cages are more closely fitted “around the stage and the core optical components” whereas the LIS ones are more bulky and put more or less a big incubator box around the entire stand. Since a few years LIS offers cages where 1/3 or 2/3 of the cage (you can choose) can be taken off easily. Especially with the 2/3 solution it is pretty easy to service most parts of the system without deinstalling the entire incubator. This is a big advantage. The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample. Concerning CO2, we use additional stage top incubation inserts on all the systems in order to provide very precise and fast onset CO2 levels in a rather small volume that is easier to control. All brands control the gas flow and mixing very nicely. However, the humidifiers turn out to be the most critical point in all gas mixers. We prefer the ones that are using more or less normal red or blue cap bottles (OkoLab PeCon) as they can be much easier refilled, especially by users. Other brands like LIS offer slim, long humidifier bottles that are positioned inside the incubator and thus have the same temp. which is very good for temp. stability. However, refilling the water can be tedious and you should be prepared that you or at least your users break at least on bottle… Since 2013 IBIDI also offers a nice gas mixer that can be added to any other incubation chamber. Concerning prices you will have to ask for offers. Prices change continuously and it is hard to tell who is cheapest or most expensive at a time… I hope that helps! Cheers, Oliver Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel Phone: +41 61 267 20 73 | Email: [hidden email] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch On 15 May 2014, at 03:15, Jeff Spector <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Greetings, > I am looking in to purchasing a stage top incubator for our spinning > disk system so we can do live cell imaging. I've looked a lot at the Tokai > Hit setup but have also heard good things about stuff from OKO lab. Do you > microscopy experts have any suggestions/advice, or opinions about what > system we should use? > > thanks! > -Jeff |
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In reply to this post by Jeff Reece
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Yes, Bioptechs has very good support ________________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Jeff Reece <[hidden email]> Sent: Thursday, May 15, 2014 12:10 AM To: [hidden email] Subject: Re: stagetop incubators ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Another worth looking at is Bioptechs.com. Kind Regards, Another Jeff From: Jeff Spector Sent: Wednesday, May 14, 2014 9:15 PM To: [hidden email] Subject: stagetop incubators ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Greetings, I am looking in to purchasing a stage top incubator for our spinning disk system so we can do live cell imaging. I've looked a lot at the Tokai Hit setup but have also heard good things about stuff from OKO lab. Do you microscopy experts have any suggestions/advice, or opinions about what system we should use? thanks! -Jeff |
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In reply to this post by Jeff Spector
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We have two Pathology Devices incubators that we are very happy with. Dave http://www.pathologydevices.com On May 14, 2014, at 9:15 PM, Jeff Spector <[hidden email]> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Greetings, I am looking in to purchasing a stage top incubator for our spinning disk system so we can do live cell imaging. I've looked a lot at the Tokai Hit setup but have also heard good things about stuff from OKO lab. Do you microscopy experts have any suggestions/advice, or opinions about what system we should use? thanks! -Jeff David Knecht, Ph.D. Professor and Head of Core Microscopy Facility Department of Molecular and Cell Biology U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax) |
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In reply to this post by Jeff Spector
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi, we are running an Ibidi system now for about 10 months and are quite pleased with it. Support (at least here) is also very quick and uncomplicated. Best, Christian Am 15.05.2014 03:15, schrieb Jeff Spector: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Greetings, > I am looking in to purchasing a stage top incubator for our spinning > disk system so we can do live cell imaging. I've looked a lot at the Tokai > Hit setup but have also heard good things about stuff from OKO lab. Do you > microscopy experts have any suggestions/advice, or opinions about what > system we should use? > > thanks! > -Jeff -- Christian Liebig, PhD Light Microscopy Facility Max-Planck-Institute for Developmental Biology Spemannstrasse 35 D-72076 Tuebingen Germany Phone: +49 7071 601 443 e-mail: [hidden email] e-mail2: [hidden email] |
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In reply to this post by Oliver Biehlmaier-2
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Oliver, Could you elaborate on the statement: “The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample.” This is counterintuitive to me, as I would think one would monitor the sample if one is trying to control its temp. Cheers, Jeff From: Oliver Biehlmaier Sent: Thursday, May 15, 2014 3:12 AM To: [hidden email] Subject: Re: stagetop incubators ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Jeff, We have several types of incubators in our facility: PeCon (comes with Zeiss systems), LIS (comes with Leica systems, at least in CH), OkoLab. All of these systems are cage incubators and offer stable and reliable temp. control. PeCon and OkoLab cages are more closely fitted “around the stage and the core optical components” whereas the LIS ones are more bulky and put more or less a big incubator box around the entire stand. Since a few years LIS offers cages where 1/3 or 2/3 of the cage (you can choose) can be taken off easily. Especially with the 2/3 solution it is pretty easy to service most parts of the system without deinstalling the entire incubator. This is a big advantage. The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample. Concerning CO2, we use additional stage top incubation inserts on all the systems in order to provide very precise and fast onset CO2 levels in a rather small volume that is easier to control. All brands control the gas flow and mixing very nicely. However, the humidifiers turn out to be the most critical point in all gas mixers. We prefer the ones that are using more or less normal red or blue cap bottles (OkoLab PeCon) as they can be much easier refilled, especially by users. Other brands like LIS offer slim, long humidifier bottles that are positioned inside the incubator and thus have the same temp. which is very good for temp. stability. However, refilling the water can be tedious and you should be prepared that you or at least your users break at least on bottle… Since 2013 IBIDI also offers a nice gas mixer that can be added to any other incubation chamber. Concerning prices you will have to ask for offers. Prices change continuously and it is hard to tell who is cheapest or most expensive at a time… I hope that helps! Cheers, Oliver Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel Phone: +41 61 267 20 73 | Email: [hidden email] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch On 15 May 2014, at 03:15, Jeff Spector <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Greetings, > I am looking in to purchasing a stage top incubator for our spinning > disk system so we can do live cell imaging. I've looked a lot at the Tokai > Hit setup but have also heard good things about stuff from OKO lab. Do you > microscopy experts have any suggestions/advice, or opinions about what > system we should use? > > thanks! > -Jeff |
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In reply to this post by Jeff Spector
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** It's not exactly along the same lines, but we've had fairly good success using the Physitemp peltier stage for live imaging. There are a couple advantages to having a Peltier stage incubator: 1) You can incubate at any temperature from -20oC to +60oC. 2) Due to the ability to both heat and cool, the stage stabilizes at any desired temperature in about 30 seconds. Disadvantage: 1) You need a nearby water supply to cool/warm the backside of the Peltier device. For CO2, we either use HEPES for up to 8 hours of live imaging, or pass a 5% CO2 blend through a bubbler for longer culture times. -Ben Smith ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of Jeff Spector [[hidden email]] Sent: Wednesday, May 14, 2014 8:15 PM To: [hidden email] Subject: stagetop incubators ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Greetings, I am looking in to purchasing a stage top incubator for our spinning disk system so we can do live cell imaging. I've looked a lot at the Tokai Hit setup but have also heard good things about stuff from OKO lab. Do you microscopy experts have any suggestions/advice, or opinions about what system we should use? thanks! -Jeff |
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In reply to this post by Jeff Reece
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Jeff, You are right. With the Okolab setup you can measure directly in your sample or “slightly indirectly” bei measuring in a reference dish in the stage top-CO2-incubator. All other incubators in our facility feature the other setup with the sensor in the cage (but not in the stage top incubator) somewhere close to the sample Cheers, Oliver Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel Phone: +41 61 267 20 73 | Email: [hidden email] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch On 15 May 2014, at 14:26, Jeff Reece <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Oliver, > > Could you elaborate on the statement: > “The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample.” > This is counterintuitive to me, as I would think one would monitor the sample if one is trying to control its temp. > > Cheers, > Jeff > > From: Oliver Biehlmaier > Sent: Thursday, May 15, 2014 3:12 AM > To: [hidden email] > Subject: Re: stagetop incubators > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Jeff, > > We have several types of incubators in our facility: PeCon (comes with Zeiss systems), LIS (comes with Leica systems, at least in CH), OkoLab. All of these systems are cage incubators and offer stable and reliable temp. control. PeCon and OkoLab cages are more closely fitted “around the stage and the core optical components” whereas the LIS ones are more bulky and put more or less a big incubator box around the entire stand. Since a few years LIS offers cages where 1/3 or 2/3 of the cage (you can choose) can be taken off easily. Especially with the 2/3 solution it is pretty easy to service most parts of the system without deinstalling the entire incubator. This is a big advantage. > The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample. > > Concerning CO2, we use additional stage top incubation inserts on all the systems in order to provide very precise and fast onset CO2 levels in a rather small volume that is easier to control. All brands control the gas flow and mixing very nicely. However, the humidifiers turn out to be the most critical point in all gas mixers. We prefer the ones that are using more or less normal red or blue cap bottles (OkoLab PeCon) as they can be much easier refilled, especially by users. Other brands like LIS offer slim, long humidifier bottles that are positioned inside the incubator and thus have the same temp. which is very good for temp. stability. However, refilling the water can be tedious and you should be prepared that you or at least your users break at least on bottle… > Since 2013 IBIDI also offers a nice gas mixer that can be added to any other incubation chamber. > > Concerning prices you will have to ask for offers. Prices change continuously and it is hard to tell who is cheapest or most expensive at a time… > > I hope that helps! > Cheers, > Oliver > > > > > Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel > Phone: +41 61 267 20 73 | Email: [hidden email] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch > > > > > On 15 May 2014, at 03:15, Jeff Spector <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Greetings, >> I am looking in to purchasing a stage top incubator for our spinning >> disk system so we can do live cell imaging. I've looked a lot at the Tokai >> Hit setup but have also heard good things about stuff from OKO lab. Do you >> microscopy experts have any suggestions/advice, or opinions about what >> system we should use? >> >> thanks! >> -Jeff |
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In reply to this post by Jeff Spector
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** The Invivo scientific we have also has the sensor at the sample. Thanks, Prabhakar -------------------------------- From: Oliver Biehlmaier <mailto:[hidden email]> Sent: 5/16/2014 9:03 AM To: [hidden email] <mailto:[hidden email]> Subject: Re: stagetop incubators ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Jeff, You are right. With the Okolab setup you can measure directly in your sample or “slightly indirectly” bei measuring in a reference dish in the stage top-CO2-incubator. All other incubators in our facility feature the other setup with the sensor in the cage (but not in the stage top incubator) somewhere close to the sample Cheers, Oliver Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel Phone: +41 61 267 20 73 | Email: [hidden email] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch On 15 May 2014, at 14:26, Jeff Reece <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Oliver, > > Could you elaborate on the statement: > “The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample.” > This is counterintuitive to me, as I would think one would monitor the sample if one is trying to control its temp. > > Cheers, > Jeff > > From: Oliver Biehlmaier > Sent: Thursday, May 15, 2014 3:12 AM > To: [hidden email] > Subject: Re: stagetop incubators > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Jeff, > > We have several types of incubators in our facility: PeCon (comes with Zeiss systems), LIS (comes with Leica systems, at least in CH), OkoLab. All of these systems are cage incubators and offer stable and reliable temp. control. PeCon and OkoLab cages are more closely fitted “around the stage and the core optical components” whereas the LIS ones are more bulky and put more or less a big incubator box around the entire stand. Since a few years LIS offers cages where 1/3 or 2/3 of the cage (you can choose) can be taken off easily. Especially with the 2/3 solution it is pretty easy to service most parts of the system without deinstalling the entire incubator. This is a big advantage. > The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample. > > Concerning CO2, we use additional stage top incubation inserts on all the systems in order to provide very precise and fast onset CO2 levels in a rather small volume that is easier to control. All brands control the gas flow and mixing very nicely. However, the humidifiers turn out to be the most critical point in all gas mixers. We prefer the ones that are using more or less normal red or blue cap bottles (OkoLab PeCon) as they can be much easier refilled, especially by users. Other brands like LIS offer slim, long humidifier bottles that are positioned inside the incubator and thus have the same temp. which is very good for temp. stability. However, refilling the water can be tedious and you should be prepared that you or at least your users break at least on bottle… > Since 2013 IBIDI also offers a nice gas mixer that can be added to any other incubation chamber. > > Concerning prices you will have to ask for offers. Prices change continuously and it is hard to tell who is cheapest or most expensive at a time… > > I hope that helps! > Cheers, > Oliver > > > > > Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel > Phone: +41 61 267 20 73 | Email: [hidden email] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch > > > > > On 15 May 2014, at 03:15, Jeff Spector <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Greetings, >> I am looking in to purchasing a stage top incubator for our spinning >> disk system so we can do live cell imaging. I've looked a lot at the Tokai >> Hit setup but have also heard good things about stuff from OKO lab. Do you >> microscopy experts have any suggestions/advice, or opinions about what >> system we should use? >> >> thanks! >> -Jeff |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Temperature can vary greatly over small distances. Therefore, temperature measured peripherally is generally not a good indicator of specimen temperature. We study heat propagation very closely. Peripheral heat transfer turns out to be the worst for warmup uniformity, speed, and regulation due to overshoot error. Once fully equilibrated box systems do work, they are also cumbersome, use CO2 quicker and slow to equilibrate new samples. Therefore, often times a combination of an environmental box and a stage-top specimen containment device provide the best solution. When it comes to temperature sensing and feedback, Bioptechs systems sense the temperature of the specimen adjacent to the specimen and incorporate the value of a sensor temporarily placed within the specimen plane used to determine the proper offset for regulation. Heat is applied in the most efficient manner (usually by electro-resistive coatings or other direct to specimen techniques) to ensure quick accurate control, no overshoot and fast recovery in the case that the specimen temperature is forced out of tolerance by external forces such as, un-equilibrated perfusion, surface evaporation, entropy, etc. This technique has enabled Bioptechs products to provide fast accurate temperature control, and high numeric aperture compatibility for all of our products. If you want to try another approach to micro-environmental control, please fill out our user profile questionnaire and return it to us and we will let you know if we have a solution. http://www.bioptechs.com/ProfileQuestions.pdf We can then arrange for you to have a demo unit. Dan On May 16, 2014, at 10:08 AM, Prabhakar Pandian wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** The Invivo scientific we have also has the sensor at the sample. Thanks, Prabhakar -------------------------------- From: Oliver Biehlmaier <mailto:[hidden email]> Sent: 5/16/2014 9:03 AM To: [hidden email] <mailto:[hidden email]> Subject: Re: stagetop incubators ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Jeff, You are right. With the Okolab setup you can measure directly in your sample or “slightly indirectly” bei measuring in a reference dish in the stage top-CO2-incubator. All other incubators in our facility feature the other setup with the sensor in the cage (but not in the stage top incubator) somewhere close to the sample Cheers, Oliver Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel Phone: +41 61 267 20 73 | Email: [hidden email] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch On 15 May 2014, at 14:26, Jeff Reece <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Oliver, > > Could you elaborate on the statement: > “The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample.” > This is counterintuitive to me, as I would think one would monitor the sample if one is trying to control its temp. > > Cheers, > Jeff > > From: Oliver Biehlmaier > Sent: Thursday, May 15, 2014 3:12 AM > To: [hidden email] > Subject: Re: stagetop incubators > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Jeff, > > We have several types of incubators in our facility: PeCon (comes with Zeiss systems), LIS (comes with Leica systems, at least in CH), OkoLab. All of these systems are cage incubators and offer stable and reliable temp. control. PeCon and OkoLab cages are more closely fitted “around the stage and the core optical components” whereas the LIS ones are more bulky and put more or less a big incubator box around the entire stand. Since a few years LIS offers cages where 1/3 or 2/3 of the cage (you can choose) can be taken off easily. Especially with the 2/3 solution it is pretty easy to service most parts of the system without deinstalling the entire incubator. This is a big advantage. > The most precise temperature control is provided by OkoLab as they measure the temp. in a liquid filled dish in the stagetop incubation chamber and not like all the others with a sensor somewhere close to the sample. > > Concerning CO2, we use additional stage top incubation inserts on all the systems in order to provide very precise and fast onset CO2 levels in a rather small volume that is easier to control. All brands control the gas flow and mixing very nicely. However, the humidifiers turn out to be the most critical point in all gas mixers. We prefer the ones that are using more or less normal red or blue cap bottles (OkoLab PeCon) as they can be much easier refilled, especially by users. Other brands like LIS offer slim, long humidifier bottles that are positioned inside the incubator and thus have the same temp. which is very good for temp. stability. However, refilling the water can be tedious and you should be prepared that you or at least your users break at least on bottle… > Since 2013 IBIDI also offers a nice gas mixer that can be added to any other incubation chamber. > > Concerning prices you will have to ask for offers. Prices change continuously and it is hard to tell who is cheapest or most expensive at a time… > > I hope that helps! > Cheers, > Oliver > > > > > Oliver Biehlmaier, PhD | Head of Imaging Core Facility | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel > Phone: +41 61 267 20 73 | Email: [hidden email] | www.biozentrum.unibas.ch | www.microscopynetwork.unibas.ch > > > > > On 15 May 2014, at 03:15, Jeff Spector <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Greetings, >> I am looking in to purchasing a stage top incubator for our spinning >> disk system so we can do live cell imaging. I've looked a lot at the Tokai >> Hit setup but have also heard good things about stuff from OKO lab. Do you >> microscopy experts have any suggestions/advice, or opinions about what >> system we should use? >> >> thanks! >> -Jeff |
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