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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear friends I'd need performing optical imsging of tea leaves... any help? Ciao Alby do not miss www.maf13.org |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Alby, I have a lot of experience with imaging of tea leaves, explain to me what you would like to do and I can give you advice. Regards, Shane On 09 Feb 2013, at 19:12, Alberto Diaspro <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear friends > I'd need performing optical imsging of tea leaves... any help? > Ciao > Alby > > do not miss www.maf13.org |
In reply to this post by Alberto Diaspro
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** I have never imaged tea leaves, but I work on Arabidopsis leaves, so perhaps I can help; what would you like to know? Enrico On Sat, Feb 9, 2013 at 10:12 AM, Alberto Diaspro <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear friends > I'd need performing optical imsging of tea leaves... any help? > Ciao > Alby > > do not miss www.maf13.org > > -- Enrico Scarpella Department of Biological Sciences University of Alberta CW-405 Biological Sciences Building Edmonton AB Canada T6G 2E9 Email: [hidden email] Tel: +1-780-4922869 Fax: +1-780-4929234 |
In reply to this post by Alberto Diaspro
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear Alby, I hope you're well. I'd be happy to help offline. All the best, George ****************************** Dr. George Littlejohn Halpin Laboratory, School of Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, EX4 4QD, UK Telephone: 01392 725544 E-mail: [hidden email] http://projects.exeter.ac.uk/ein/ https://twitter.com/geolittlejohn ________________________________________ From: Confocal Microscopy List [[hidden email]] on behalf of Alberto Diaspro [[hidden email]] Sent: Saturday, February 09, 2013 5:12 PM To: [hidden email] Subject: tea leaves ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear friends I'd need performing optical imsging of tea leaves... any help? Ciao Alby do not miss www.maf13.org |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** A good old scanner can be the answer here, I have used that previously to create color images with great detail, and from that calculate foliar surface. I hope this helps. Gabriel OH > Date: Mon, 11 Feb 2013 12:55:08 +0000 > From: [hidden email] > Subject: Re: tea leaves > To: [hidden email] > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear Alby, > > I hope you're well. > I'd be happy to help offline. > > All the best, > George > > ****************************** > Dr. George Littlejohn > Halpin Laboratory, > School of Biosciences, > University of Exeter, > Geoffrey Pope Building, > Stocker Road, > EX4 4QD, UK > > Telephone: 01392 725544 > E-mail: [hidden email] > > http://projects.exeter.ac.uk/ein/ > https://twitter.com/geolittlejohn > > ________________________________________ > From: Confocal Microscopy List [[hidden email]] on behalf of Alberto Diaspro [[hidden email]] > Sent: Saturday, February 09, 2013 5:12 PM > To: [hidden email] > Subject: tea leaves > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear friends > I'd need performing optical imsging of tea leaves... any help? > Ciao > Alby > > do not miss www.maf13.org |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** If you want to see an example of a scanner's performance, I just tried a cheap scanner https://www.google.com/shopping/product/653414317389359974?q=canoscan+lide+700f on an insect wing. I had all the auto adjust features turned off. I also scanned a micrometer resolution slide to get an idea how much detail it really records. Depending how clean you need the images it may require flatfield correction or fft filtering, but it's probably no worse than one of the new sCMOS cameras. :) pics here: http://www.flickr.com/photos/mcammer/8468513938/sizes/k/ or http://www.flickr.com/photos/mcammer/8468513938/ and http://www.flickr.com/photos/mcammer/8468924638/sizes/l/ or http://www.flickr.com/photos/mcammer/8468924638/ ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Zac Arrac Atelaz Sent: Monday, February 11, 2013 1:00 PM To: [hidden email] Subject: Re: tea leaves ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** A good old scanner can be the answer here, I have used that previously to create color images with great detail, and from that calculate foliar surface. I hope this helps. Gabriel OH > Date: Mon, 11 Feb 2013 12:55:08 +0000 > From: [hidden email] > Subject: Re: tea leaves > To: [hidden email] > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear Alby, > > I hope you're well. > I'd be happy to help offline. > > All the best, > George > > ****************************** > Dr. George Littlejohn > Halpin Laboratory, > School of Biosciences, > University of Exeter, > Geoffrey Pope Building, > Stocker Road, > EX4 4QD, UK > > Telephone: 01392 725544 > E-mail: [hidden email] > > http://projects.exeter.ac.uk/ein/ > https://twitter.com/geolittlejohn > > ________________________________________ > From: Confocal Microscopy List [[hidden email]] on > behalf of Alberto Diaspro [[hidden email]] > Sent: Saturday, February 09, 2013 5:12 PM > To: [hidden email] > Subject: tea leaves > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear friends > I'd need performing optical imsging of tea leaves... any help? > Ciao > Alby > > do not miss www.maf13.org |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello Michael, Can you tell me where you purchased the sizing grids you placed on the scanner? Thank you, Nancy -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cammer, Michael Sent: Tuesday, February 12, 2013 1:37 PM To: [hidden email] Subject: Re: tea leaves ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** If you want to see an example of a scanner's performance, I just tried a cheap scanner https://www.google.com/shopping/product/653414317389359974?q=canoscan+lide+700f on an insect wing. I had all the auto adjust features turned off. I also scanned a micrometer resolution slide to get an idea how much detail it really records. Depending how clean you need the images it may require flatfield correction or fft filtering, but it's probably no worse than one of the new sCMOS cameras. :) pics here: http://www.flickr.com/photos/mcammer/8468513938/sizes/k/ or http://www.flickr.com/photos/mcammer/8468513938/ and http://www.flickr.com/photos/mcammer/8468924638/sizes/l/ or http://www.flickr.com/photos/mcammer/8468924638/ ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Zac Arrac Atelaz Sent: Monday, February 11, 2013 1:00 PM To: [hidden email] Subject: Re: tea leaves ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** A good old scanner can be the answer here, I have used that previously to create color images with great detail, and from that calculate foliar surface. I hope this helps. Gabriel OH > Date: Mon, 11 Feb 2013 12:55:08 +0000 > From: [hidden email] > Subject: Re: tea leaves > To: [hidden email] > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear Alby, > > I hope you're well. > I'd be happy to help offline. > > All the best, > George > > ****************************** > Dr. George Littlejohn > Halpin Laboratory, > School of Biosciences, > University of Exeter, > Geoffrey Pope Building, > Stocker Road, > EX4 4QD, UK > > Telephone: 01392 725544 > E-mail: [hidden email] > > http://projects.exeter.ac.uk/ein/ > https://twitter.com/geolittlejohn > > ________________________________________ > From: Confocal Microscopy List [[hidden email]] on > behalf of Alberto Diaspro [[hidden email]] > Sent: Saturday, February 09, 2013 5:12 PM > To: [hidden email] > Subject: tea leaves > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear friends > I'd need performing optical imsging of tea leaves... any help? > Ciao > Alby > > do not miss www.maf13.org |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** http://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=4338 Be very careful when you put glass on the scanner not to scratch the scanner glass. Regards, Michael -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Hirdt, Nancy {PCNA} Sent: Tuesday, February 12, 2013 4:38 PM To: [hidden email] Subject: Re: tea leaves ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hello Michael, Can you tell me where you purchased the sizing grids you placed on the scanner? Thank you, Nancy -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Cammer, Michael Sent: Tuesday, February 12, 2013 1:37 PM To: [hidden email] Subject: Re: tea leaves ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** If you want to see an example of a scanner's performance, I just tried a cheap scanner https://www.google.com/shopping/product/653414317389359974?q=canoscan+lide+700f on an insect wing. I had all the auto adjust features turned off. I also scanned a micrometer resolution slide to get an idea how much detail it really records. Depending how clean you need the images it may require flatfield correction or fft filtering, but it's probably no worse than one of the new sCMOS cameras. :) pics here: http://www.flickr.com/photos/mcammer/8468513938/sizes/k/ or http://www.flickr.com/photos/mcammer/8468513938/ and http://www.flickr.com/photos/mcammer/8468924638/sizes/l/ or http://www.flickr.com/photos/mcammer/8468924638/ ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Zac Arrac Atelaz Sent: Monday, February 11, 2013 1:00 PM To: [hidden email] Subject: Re: tea leaves ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** A good old scanner can be the answer here, I have used that previously to create color images with great detail, and from that calculate foliar surface. I hope this helps. Gabriel OH > Date: Mon, 11 Feb 2013 12:55:08 +0000 > From: [hidden email] > Subject: Re: tea leaves > To: [hidden email] > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear Alby, > > I hope you're well. > I'd be happy to help offline. > > All the best, > George > > ****************************** > Dr. George Littlejohn > Halpin Laboratory, > School of Biosciences, > University of Exeter, > Geoffrey Pope Building, > Stocker Road, > EX4 4QD, UK > > Telephone: 01392 725544 > E-mail: [hidden email] > > http://projects.exeter.ac.uk/ein/ > https://twitter.com/geolittlejohn > > ________________________________________ > From: Confocal Microscopy List [[hidden email]] on > behalf of Alberto Diaspro [[hidden email]] > Sent: Saturday, February 09, 2013 5:12 PM > To: [hidden email] > Subject: tea leaves > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear friends > I'd need performing optical imsging of tea leaves... any help? > Ciao > Alby > > do not miss www.maf13.org |
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