tiny filters

[Martin Köhler]

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Dear confocalist,

I am looking for a suitable source of tiny bandpass filters. Small diameter and small thickness. Diameter down to 1 mm or maybe even less, and thickness below 1 mm. Probably these items have to be custom made. But there are many manufacturers for optical filters on the market, and therefore I wanted to hear if you can give any recommendations to me about suitable companies for this.

Thank you,
Martin

------------------------------------------------------------
 Martin Köhler, PhD
 The Rolf Luft Research Center for Diabetes and Endocrinology
 Karolinska Institutet
 Karolinska University Hospital Solna L1
 SE-171 76  Stockholm, Sweden

 E-mail:  [hidden email]
 Phone:   [phone]
 Cell:    [phone]
 ------------------------------------------------------------

[Jens]

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Hi Martin,

Depending on the wavelength you need, old DVD or blueray drives might be a solution.

Jens

Sent from my iPhone

> On 15 Mar 2017, at 15:21, Martin Köhler <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Dear confocalist,
>
> I am looking for a suitable source of tiny bandpass filters. Small diameter and small thickness. Diameter down to 1 mm or maybe even less, and thickness below 1 mm. Probably these items have to be custom made. But there are many manufacturers for optical filters on the market, and therefore I wanted to hear if you can give any recommendations to me about suitable companies for this.
>
> Thank you,
> Martin
>
> ------------------------------------------------------------
>  Martin Köhler, PhD
>  The Rolf Luft Research Center for Diabetes and Endocrinology
>  Karolinska Institutet
>  Karolinska University Hospital Solna L1
>  SE-171 76  Stockholm, Sweden
>
>  E-mail:  [hidden email]
>  Phone:   [phone]
>  Cell:    [phone]
>  ------------------------------------------------------------
>

[Martin Wessendorf-2]

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Dear Dr. Köhler--

Would any of the colored glasses from Schott work for you?  The
disadvantage is that optical density will be a function of thickness but
the advantage would be the ability to fabricate the glass into any shape
that you might want.

http://www.us.schott.com/advanced_optics/english/filter/us/index.html

Good luck!

Martin Wessendorf




On 3/15/2017 9:21 AM, Martin Köhler wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Dear confocalist,
>
> I am looking for a suitable source of tiny bandpass filters. Small diameter and small thickness. Diameter down to 1 mm or maybe even less, and thickness below 1 mm. Probably these items have to be custom made. But there are many manufacturers for optical filters on the market, and therefore I wanted to hear if you can give any recommendations to me about suitable companies for this.
>
> Thank you,
> Martin
>
> ------------------------------------------------------------
>   Martin Köhler, PhD
>   The Rolf Luft Research Center for Diabetes and Endocrinology
>   Karolinska Institutet
>   Karolinska University Hospital Solna L1
>   SE-171 76  Stockholm, Sweden
>
>   E-mail:  [hidden email]
>   Phone:   [phone]
>   Cell:    [phone]
>   ------------------------------------------------------------
>

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[George McNamara]

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Hi Martin,

Please email Chroma and Semrock. I believe the tele-communications
industry used small diameter filters.

Web links to these two, and lots of other filter companies, in the first
section of

http://www.geomcnamara.com/light-microscopy-websites


Sincerely,

George



On 3/15/2017 9:21 AM, Martin Köhler wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Dear confocalist,
>
> I am looking for a suitable source of tiny bandpass filters. Small diameter and small thickness. Diameter down to 1 mm or maybe even less, and thickness below 1 mm. Probably these items have to be custom made. But there are many manufacturers for optical filters on the market, and therefore I wanted to hear if you can give any recommendations to me about suitable companies for this.
>
> Thank you,
> Martin
>
> ------------------------------------------------------------
>   Martin Köhler, PhD
>   The Rolf Luft Research Center for Diabetes and Endocrinology
>   Karolinska Institutet
>   Karolinska University Hospital Solna L1
>   SE-171 76  Stockholm, Sweden
>
>   E-mail:  [hidden email]
>   Phone:   [phone]
>   Cell:    [phone]
>   ------------------------------------------------------------
>

--




George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75/
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650

[George McNamara]

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Dear confocal listserv, Want money? Work on cells?

Pilot Projects for a Human Cell Atlas ... request for applications
posted by Chan Zuckerberg Initiative

https://chanzuckerberg.com/initiatives/rfa

REQUEST FOR APPLICATIONS

Pilot Projects for a Human Cell Atlas

OPPORTUNITY

The goal of the International Human Cell Atlas project is to create a
reference atlas of all cells in the healthy human body as a resource for
studies of health and disease. As part of this effort, the Chan
Zuckerberg Initiative seeks applications to support the Human Cell Atlas
community’s convergence around standards, protocols, and best practices
for the creation of a freely and openly available reference database of
healthy human cells and tissues.

Project Specifications

We expect the reference Cell Atlas to ultimately include a variety of
data types, including single-cell RNA sequencing, in situ hybridization
imaging, proteomics, metabolomics, epigenetic information, low- and
high-resolution images of biological samples, and more. For the initial
phases of the project, we are especially interested in groups that can
help evaluate protocols and best practices for the collection of
single-cell molecular data, ideally by directly comparing multiple
approaches to tissue preparation, data generation, or data analysis.

Example pilot project goals might include, but are not limited to:

Systematic comparisons of tissue preparation and preservation, sample
handling, and cell dissociation approaches for a variety of human
tissues and organs.
Systematic comparisons of sequencing technologies, protocols, and
resulting datasets.
Evaluation of experimental replicability, including comparing different
experimental procedures within the same lab, and similar procedures
across labs.
Comparison of image-based approaches, such as single-molecule
fluorescent in situ hybridization imaging (smFISH), with
sequencing-based approaches.
Comparison of different multiplexing strategies for image-based approaches.
Evaluating suitability of experimental strategies to different
computational pipelines.
Evaluation of biological replicability depending on donor age, sex, or
tissue type.
Evaluation of multiple tissues from one individual, compared to
different individuals.
Generation of new benchmark or validation datasets.
We are primarily interested in assembling a broad and biologically
compelling set of human tissues for the reference atlas, but in this
pilot phase, we will also consider proposals for obtaining data from
cell lines, organoids, or non-human animals.


//

If you lack imagination, you can check out my post HCA-Stanford meeting
post for ideas for you to spin to your needs.

https://www.linkedin.com/pulse/comprehensive-binders-proposal-more-george-mcnamara



Sincerely,
George


George McNamara, PhD
Houston, TX 77054
[hidden email]
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75/
http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650

[Jeffrey Carmichael]

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*COMMERCIAL RESPONSE*

Hello Martin,

We can do this with certain constraints regarding filter shape and the size
of the required clear aperture.

Please feel free to contact me.

Best,
Jeff



*Jeff Carmichael*

*Technical and Product Marketing Manager*

*[hidden email] <[hidden email]>*Chroma Technology Corp.

*an employee owned company*
*10 Imtec Lane*
*Bellows Falls, VT  05301*
*802-428-2528 Office*
*802-428-2528 Fax**800-824-7662 Toll Free*

On Wed, Mar 15, 2017 at 10:21 AM, Martin Köhler <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Dear confocalist,
>
> I am looking for a suitable source of tiny bandpass filters. Small
> diameter and small thickness. Diameter down to 1 mm or maybe even less, and
> thickness below 1 mm. Probably these items have to be custom made. But
> there are many manufacturers for optical filters on the market, and
> therefore I wanted to hear if you can give any recommendations to me about
> suitable companies for this.
>
> Thank you,
> Martin
>
> ------------------------------------------------------------
>  Martin Köhler, PhD
>  The Rolf Luft Research Center for Diabetes and Endocrinology
>  Karolinska Institutet
>  Karolinska University Hospital Solna L1
>  SE-171 76  Stockholm, Sweden
>
>  E-mail:  [hidden email]
>  Phone:   [phone]
>  Cell:    [phone]
>  ------------------------------------------------------------
>
>

[Christian Feldhaus]

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*****

Hi,

we had filters cut to custom size by AHF (www.ahf.de). They've been very
helpful and we were happy with the result.

Best,

Christian



On 15.03.2017 15:21, Martin Köhler wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Dear confocalist,
>
> I am looking for a suitable source of tiny bandpass filters. Small diameter and small thickness. Diameter down to 1 mm or maybe even less, and thickness below 1 mm. Probably these items have to be custom made. But there are many manufacturers for optical filters on the market, and therefore I wanted to hear if you can give any recommendations to me about suitable companies for this.
>
> Thank you,
> Martin
>
> ------------------------------------------------------------
>   Martin Köhler, PhD
>   The Rolf Luft Research Center for Diabetes and Endocrinology
>   Karolinska Institutet
>   Karolinska University Hospital Solna L1
>   SE-171 76  Stockholm, Sweden
>
>   E-mail:  [hidden email]
>   Phone:   [phone]
>   Cell:    [phone]
>   ------------------------------------------------------------
>

--
========================================

Christian Liebig, PhD
Light Microscopy Facility
Max Planck Institute for Developmental Biology
Spemannstraße 35
72076 Tübingen
Germany
Tel: [phone]
Fax: [phone]
[hidden email]

[Ralf Palmisano]

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*****

Dear all,

we want to upgrade our Abberior STED on an Olympus stand with the
Olympus IX3-ZDC-12 z-drift compensation unit. The system is managed with
the Imspector software.
There is some sort of prize gap between Olympus and Abberior, in a
rather unreasonable manner.
We would like to get the unit built into our microscope stand directly
from Olympus. Does anyone have experience on the neccessary improvments,
which then will have to be done within the Imspector software, as
Olympus does not deliver that.

Many thanks for everey valuable input .

Cheers, Ralf

--
Ralf Palmisano
Head - Optical Imaging Centre Erlangen

Hartmannstr. 14
91052 Erlangen, Germany
[phone] (Office)
[phone] (Secretary)
[phone] (Fax)

www.oice.uni-erlangen.de

[Magdalena Jaklewicz]

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*****

Hello,

The power supply box for the Argon laser on our old Olympus Fluoview 300
system exploded.
In order to check the laser condition I need this box but I was told by
Olympus it is not available from the vendor : Melles Griot.

Could anybody lend me a unit to test the laser? Or sell/donate one ( I
will cover the shipping charges of course).

cheers and have a lovely day

Magdalena

ps - please contact me at [hidden email]


Magdalena Jaklewicz, Ph.D.
Microscopy Technologist

YORK UNIVERSITY
Faculty of Science
Life Sciences Building ? Room 429c ? 4700 Keele Street
Toronto ON ? Canada M3J 1P3
Cell: [phone]  (voice text)
F: [phone]
E: [hidden email]




From:   Ralf Palmisano OICE <[hidden email]>
To:     [hidden email]
Date:   03/20/2017 05:23 AM
Subject:        Z-drift compensation Olympus & Imspector
Sent by:        Confocal Microscopy List
<[hidden email]>



*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear all,

we want to upgrade our Abberior STED on an Olympus stand with the
Olympus IX3-ZDC-12 z-drift compensation unit. The system is managed with
the Imspector software.
There is some sort of prize gap between Olympus and Abberior, in a
rather unreasonable manner.
We would like to get the unit built into our microscope stand directly
from Olympus. Does anyone have experience on the neccessary improvments,
which then will have to be done within the Imspector software, as
Olympus does not deliver that.

Many thanks for everey valuable input .

Cheers, Ralf

--
Ralf Palmisano
Head - Optical Imaging Centre Erlangen

Hartmannstr. 14
91052 Erlangen, Germany
[phone] (Office)
[phone] (Secretary)
[phone] (Fax)

www.oice.uni-erlangen.de