transmitted light imaging

[Jeremy Adler-4]

A problem of using multiwell plates is that the liquid forms a meniscus at the edges of the well - which messes up transmitted light images.
Does anyone know of a coating that when applied to the sides of a plastic well would make its surface tension equal to that of water - thereby eliminating  the meniscus.
Or is it possible to change the surface tension of the liquid ?

Jeremy Adler
Uppsala U

[Christopher Yip]

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Not a user of these dishes but Stem Cell Technologies has meniscus-free plates:

http://www.stemcell.com/en/Products/Product-Type/Other-cell-culture-media-reagents-supplies/SmartDish.aspx

> On May 20, 2016, at 11:13 AM, Jeremy Adler <[hidden email]> wrote:
>
>
> A problem of using multiwell plates is that the liquid forms a meniscus at the edges of the well - which messes up transmitted light images.
> Does anyone know of a coating that when applied to the sides of a plastic well would make its surface tension equal to that of water - thereby eliminating  the meniscus.
> Or is it possible to change the surface tension of the liquid ?
>
> Jeremy Adler
> Uppsala U

[mcammer]

Maybe a wetting agent like PhotoFlo 200?  Or a layer of mineral oil over the top.

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 Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jeremy Adler
Sent: Friday, May 20, 2016 11:13 AM
To: [hidden email]
Subject: transmitted light imaging


A problem of using multiwell plates is that the liquid forms a meniscus at the edges of the well - which messes up transmitted light images.
Does anyone know of a coating that when applied to the sides of a plastic well would make its surface tension equal to that of water - thereby eliminating  the meniscus.
Or is it possible to change the surface tension of the liquid ?

Jeremy Adler
Uppsala U

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