water retaining container for dipping lens

[Hendrik Deschout]

Dear all,

We would like to image samples immersed in water with a water dipping  
objective on an inverted microscope. Does anyone have experience in  
fabricating a kind of water retaining container that can be fitted on  
the barrel of the objective? Or maybe something similar is  
commercially available?

Thanks in advance,

Hendrik



Hendrik Deschout
Biophotonic Imaging Group
Lab. General Biochemistry and Physical Pharmacy
Ghent University
Harelbekestraat 72
9000 Gent
Belgium
Tel: [phone]
Fax: [phone]
[hidden email]

[Guy Cox-2]

We have fabricated such chambers using silicone rubber dental modelling
compound (which is also used in our lab for SEM replica work).  It works
well, doesn't leak and can easily be removed without damage.  (It can't
be replaced, though, you have to cast a new one).  The compound is
available from microscopy suppliers such as Agar Scientific etc.

                                            Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone [phone]     Fax [phone]
             Mobile [phone]
______________________________________________
      http://www.guycox.net
 

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Hendrik Deschout
Sent: Thursday, 1 July 2010 5:39 PM
To: [hidden email]
Subject: water retaining container for dipping lens

Dear all,

We would like to image samples immersed in water with a water dipping  
objective on an inverted microscope. Does anyone have experience in  
fabricating a kind of water retaining container that can be fitted on  
the barrel of the objective? Or maybe something similar is  
commercially available?

Thanks in advance,

Hendrik



Hendrik Deschout
Biophotonic Imaging Group
Lab. General Biochemistry and Physical Pharmacy
Ghent University
Harelbekestraat 72
9000 Gent
Belgium
Tel: [phone]
Fax: [phone]
[hidden email]

No virus found in this incoming message.
Checked by AVG - www.avg.com
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04:38:00

[Steffen Dietzel]

Hendrik,

do you mean a storage tank for water or something to stop water
flowing into the revolver?
Just today, I got an advertisment from Leica on my table for some
de-luxe storage tank with automatic refueling. They call it "Water
Immersion Micro Dispenser". It's googleable.
I have no idea how well it works nor any commericial affiliation with
Leica, other than a customer.

Steffen



At 09:38 01.07.2010, you wrote:

>Dear all,
>
>We would like to image samples immersed in water with a water dipping
>objective on an inverted microscope. Does anyone have experience in
>fabricating a kind of water retaining container that can be fitted on
>the barrel of the objective? Or maybe something similar is
>commercially available?
>
>Thanks in advance,
>
>Hendrik
>
>
>
>Hendrik Deschout
>Biophotonic Imaging Group
>Lab. General Biochemistry and Physical Pharmacy
>Ghent University
>Harelbekestraat 72
>9000 Gent
>Belgium
>Tel: [phone]
>Fax: [phone]
>[hidden email]

[Sylvie Le Guyader-2]

Olympus also has the same device as Leica. To my knowledge they are the only
2 companies to offer this.

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Live Cell Imaging Unit
Dept of Biosciences and Nutrition
Karolinska Institutet
Sweden
office: [phone]
mobile: [phone]

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]] On Behalf Of Steffen
> Dietzel
> Sent: 02 July 2010 14:52
> To: [hidden email]
> Subject: Re: water retaining container for dipping lens
>
> Hendrik,
>
> do you mean a storage tank for water or something to stop water
> flowing into the revolver?
> Just today, I got an advertisment from Leica on my table for some
> de-luxe storage tank with automatic refueling. They call it "Water
> Immersion Micro Dispenser". It's googleable.
> I have no idea how well it works nor any commericial affiliation with
> Leica, other than a customer.
>
> Steffen
>
>
>
> At 09:38 01.07.2010, you wrote:
> >Dear all,
> >
> >We would like to image samples immersed in water with a water dipping
> >objective on an inverted microscope. Does anyone have experience in
> >fabricating a kind of water retaining container that can be fitted on
> >the barrel of the objective? Or maybe something similar is
> >commercially available?
> >
> >Thanks in advance,
> >
> >Hendrik
> >
> >
> >
> >Hendrik Deschout
> >Biophotonic Imaging Group
> >Lab. General Biochemistry and Physical Pharmacy
> >Ghent University
> >Harelbekestraat 72
> >9000 Gent
> >Belgium
> >Tel: [phone]
> >Fax: [phone]
> >[hidden email]

[Hendrik Deschout]

Dear Steffen,

I actually mean both. The container should store the water with the
sample immersed in it. And there should be an opening at the bottom,
where the dipping lens comes in. To prevent the water from flowing into
the revolver, this opening should somehow fit tightly around the lens
barrel.

The Leica dispenser looks kind of similar to what I mean, but I'm not
sure if it suits our application since they are only talking about
immersion (and we use Nikon lenses), but I will check it out.

Thanks for your advice,

Hendrik


Steffen Dietzel wrote:

> Hendrik,
>
> do you mean a storage tank for water or something to stop water
> flowing into the revolver?
> Just today, I got an advertisment from Leica on my table for some
> de-luxe storage tank with automatic refueling. They call it "Water
> Immersion Micro Dispenser". It's googleable.
> I have no idea how well it works nor any commericial affiliation with
> Leica, other than a customer.
>
> Steffen
>
>
>
> At 09:38 01.07.2010, you wrote:
>> Dear all,
>>
>> We would like to image samples immersed in water with a water dipping
>> objective on an inverted microscope. Does anyone have experience in
>> fabricating a kind of water retaining container that can be fitted on
>> the barrel of the objective? Or maybe something similar is
>> commercially available?
>>
>> Thanks in advance,
>>
>> Hendrik
>>
>>
>>
>> Hendrik Deschout
>> Biophotonic Imaging Group
>> Lab. General Biochemistry and Physical Pharmacy
>> Ghent University
>> Harelbekestraat 72
>> 9000 Gent
>> Belgium
>> Tel: [phone]
>> Fax: [phone]
>> [hidden email]

[Stephen Cody-2]

Dear Hendrick,

Sounds like a job for my now infamous condom bath. Search the archives for condon. And check out the paper: 

Cody and Williams Journal of Microscopy 185(1): 94-97, (1997).

Cheers 

Steve

Safe microscopy!

Stephen H. Cody

On 02/07/2010, at 11:27 PM, Hendrik Deschout <[hidden email]> wrote:

Dear Steffen,

I actually mean both. The container should store the water with the sample immersed in it. And there should be an opening at the bottom, where the dipping lens comes in. To prevent the water from flowing into the revolver, this opening should somehow fit tightly around the lens barrel.

The Leica dispenser looks kind of similar to what I mean, but I'm not sure if it suits our application since they are only talking about immersion (and we use Nikon lenses), but I will check it out.

Thanks for your advice,

Hendrik


Steffen Dietzel wrote:

Hendrik,

do you mean a storage tank for water or something to stop water flowing into the revolver?

Just today, I got an advertisment from Leica on my table for some de-luxe storage tank with automatic refueling. They call it "Water Immersion Micro Dispenser". It's googleable.

I have no idea how well it works nor any commericial affiliation with Leica, other than a customer.

Steffen

At 09:38 01.07.2010, you wrote:

Dear all,

We would like to image samples immersed in water with a water dipping

objective on an inverted microscope. Does anyone have experience in

fabricating a kind of water retaining container that can be fitted on

the barrel of the objective? Or maybe something similar is

commercially available?

Thanks in advance,

Hendrik

Hendrik Deschout

Biophotonic Imaging Group

Lab. General Biochemistry and Physical Pharmacy

Ghent University

Harelbekestraat 72

9000 Gent

Belgium

Tel: [phone]

Fax: [phone]

[hidden email]