Frame grabber for Hamamatsu CCD ORCA-ER
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Lingqing Zhang |
Frame grabber for Hamamatsu CCD ORCA-ER
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Dear Lister,
Could somebody recommend a frame grabber to work with Hamamatsu digital camera ORCA-ER? Also a data acquisition software (free ones will be better) if frame grabber does not provide one. Thank you very much for your help. Lingqing *********************************************** Lingqing Zhang, PhD Nonlinear Optical Imaging Lab Sensory Neuroscience Research Center West Virginia University 1 Medical Center Drive Morgantown, WV 26506 Phone: 304-293-5253 Email: [hidden email] *********************************************** |
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Hi Lingqing,
We simply use our ORCA-ER with a firewire card via the 1394 port; we use MicroManager for data acquisition, which is free and simple to use.
Zoltan
On Fri, Jun 26, 2009 at 3:55 PM, Lingqing Zhang <[hidden email]> wrote: Dear Lister, -- Zoltan Cseresnyes Facility manager, Imaging Suite |
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Theresa Swayne |
Re: Frame grabber for Hamamatsu CCD ORCA-ER
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In reply to this post by Lingqing Zhang
Hope this helps. Theresa
On Jun 26, 2009, at 10:55 AM, Lingqing Zhang wrote: Could somebody recommend a frame grabber to work with Hamamatsu digital camera ORCA-ER? Also a data acquisition software (free ones will be better) if frame grabber does not provide one. ------------------------------------ Theresa C. Swayne, Ph.D. Manager, Confocal and Specialized Microscopy Shared Resource Herbert Irving Comprehensive Cancer Center, Columbia University 1130 Saint Nicholas Ave, 222A New York, NY 10032 212-851-4613 |
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Haberman, Ann |
thoughts on dispersion compensation?
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In reply to this post by Zoltan
Hello List!
I am considering upgrading my Ti:Sapphire laser to one that will provide dispersion compensation of the beam before it hits the microscope. I am hoping that someone who has purchased such a pre-compensation module for their Coherent or Spectra Physics laser might comment on their experience. My current microscope configuration uses a fixed prism to provide negative group velocity dispersion. If I understand correctly, this will only tighten the pulse for a narrow range of laser wavelengths. The pre-compensation units that are supplied by the laser manufacturers are reputed to adjust the amount of correction according to laser wavelength. Is this true or is the same amount of compensation applied to all wavelengths? Has anyone been in a position to compare a fixed prism arrangement to one that is integrated with the laser system? Do you feel that it has improved your ability to excite fluorophores at wavelengths that were less efficient previously with a fixed prism, or did the improvement seem negligible? Thanks in advance for sharing your experience, Ann Haberman -- Ann Haberman, PhD In Vivo Imaging Facility, Director Department of Laboratory Medicine Yale University School of Medicine 300 Cedar Street TAC S541 New Haven, CT 06510 203-785-7349 203-785-5415 (fax) [hidden email] |
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Jerry Sedgewick-2 |
Re: thoughts on dispersion compensation?
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I have added a FEMTO pre-compensation unit to the laser path (no
commercial interest). This unit moves prisms via motors to eliminate issues with the laser moving as wavelengths are changed. A big problem that occurs when prisms don't move is that of the laser walking when wavelengths are changed. I did have a fixed set of prisms optimized at 820nm, and then purchased a commercial unit (the FEMTO). Certainly, it is not as convenient as having the pre-compensation apparatus combined with the Ti:Sapphire (as in the DeepSee) because you have to remember to move the prisms using the control box, and one more thing to remember is easy to forget. But, I was able to use the existing Ti:Sapphire and provide pre-compensation. I know that more than one company makes the motorized pre-compensation units: I went with the advice of the faculty member who funded the unit. This particular unit allows one to choose the amount of compensation at each wavelength. I am not familiar with how Coherent or Spectraphysics lasers provide correction, and whether or not there is means for overriding settings, if necessary, or selecting from among a series of choices for the level of correction. As far as the ability to excite fluorophores, this doesn't change: it's the ability to achieve greater depth in the sample that changes. For my custom multiphoton, the pre-compensation didn't make a huge difference (about 50 - 75 extra microns in lymph nodes). Perhaps that is because I don't use silver mirrors; instead I use dichroics for guiding the laser line, and so the dispersion is minimized (though most of this occurs at lenses). Also, one can create a spot that is smaller than the back aperture of the objective to gain power and depth while sacrificing resolution by adding a planoconvex lens at 500 to 1000mm focal lengths. Depending on what is imaged, a sacrifice of resolution might be a reasonable trade off. Jerry Ann Haberman wrote: > Hello List! > > I am considering upgrading my Ti:Sapphire laser to one that will > provide dispersion compensation of the beam before it hits the > microscope. I am hoping that someone who has purchased such a > pre-compensation module for their Coherent or Spectra Physics laser > might comment on their experience. > > My current microscope configuration uses a fixed prism to provide > negative group velocity dispersion. If I understand correctly, this > will only tighten the pulse for a narrow range of laser wavelengths. > > The pre-compensation units that are supplied by the laser > manufacturers are reputed to adjust the amount of correction according > to laser wavelength. Is this true or is the same amount of > compensation applied to all wavelengths? > > Has anyone been in a position to compare a fixed prism arrangement to > one that is integrated with the laser system? Do you feel that it has > improved your ability to excite fluorophores at wavelengths that were > less efficient previously with a fixed prism, or did the improvement > seem negligible? > > Thanks in advance for sharing your experience, > Ann Haberman > > -- Jerry Sedgewick Sedgewick Initiatives 965 Cromwell Avenue Saint Paul, MN 55114 651-788-2261 [hidden email] http://www.rawlight.com http://www.jerrysedgewick.com --- Get FREE High Speed Internet from USFamily.Net! -- http://www.usfamily.net/mkt-freepromo.html --- |
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Andreas Bruckbauer |
Beam pointing stability MaiTai Deepsee on Zeiss LSM 710, pre-chirp
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Thanks to the combined efforts of the local Spectraphysics and Zeiss engineers the illumination profile is now centered over the whole wavelength range (700 - 1040nm) and does not wander any more as i previously reported, it is worth checkking this with a fluorescent slide.
To the pre-chirp with the spectraphysics deepsee: Yes it is possible and highly recommended to create your own table(s) for the prism position with wavelength, they have to be inside some "soft limits" though. Then everything works automatically unless the cable at the deepsee unit is unplugged (in this case you need to restart the laser). We were able to upgrade an existing MaiTai with a deepsee unit. The pre-chirp allows to use lower average laser power to generate the same 2-photon signal, I think it depends on the individual conditions if this really translates into more penetration depth. best wishes Andreas Click here to get the very best of AOL, including news, sport, gossip, lifestyles updates and email. |
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Lingqing Zhang |
Re: Frame grabber for Hamamatsu CCD ORCA-ER
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In reply to this post by Theresa Swayne
Theresa,
Sorry for so late response. Just back from Vacation.
Thank you very much for your help. I think I am going to try firewire + MicroManager combination first as Zoltan suggested.
Lingqing
On Fri, Jun 26, 2009 at 12:19 PM, Theresa Swayne <[hidden email]> wrote:
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Lingqing Zhang |
Re: Frame grabber for Hamamatsu CCD ORCA-ER
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In reply to this post by Zoltan
Zoltan,,
Sorry for so late response. Just back from Vacation.
Great suggestion! Thank you very much for your help.
Lingqing
On Fri, Jun 26, 2009 at 11:08 AM, Zoltan Cseresnyes <[hidden email]> wrote:
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Nico Stuurman |
Re: Frame grabber for Hamamatsu CCD ORCA-ER
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In reply to this post by Lingqing Zhang
Hi Lingqing,
Please note that you do not really have a choice whether to use firewire or a framegrabber card. It depends on the type of ORCA ER you have. Hamamatsu sells cameras with firewire interface and cameras with cameralink interface (that need the Snapper or Phoenix cards). Micro-Manager will work with both (as it uses the Hamamatsu DCAM interface that works with both). Best, Nico
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Knecht, David |
Re: Frame grabber for Hamamatsu CCD ORCA-ER
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Nico- I have the Orca with a Snapper card and an earlier post indicated that this would not work with micro-manager. I haven't tried it myself. Do you know if it is true? Dave
On Jul 6, 2009, at 2:43 PM, Nico Stuurman wrote:
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax) |
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Nico Stuurman |
Re: Frame grabber for Hamamatsu CCD ORCA-ER
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The list of hardware supported by DCAM can be found here: http://sales.hamamatsu.com/index.php?id=13188404 It indeed looks like the Snapper card is not on that list (although not sure, best to check the exact make and model of the card you have). Sorry for the confusion. Best, Nico
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