Indo with multiphoton question
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dear multiphotoners, I'm planning to try using Indo on the multiphoton microscope next week and was wondering whether anybody has tips on best wavelengths to use for excitation (e.g. 720 or 920 nm?) or anything else of potential use. Thank you! Sincerely, Michael ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270 </PRE> <html> <body> ------------------------------------------------------------<br /> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> ================================= </body> </html> <PRE> |
 
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Paul Herzmark |
Re: Indo with multiphoton question
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Michael, We do it here with the laser tuned to 720. We separate the two Indo peaks into two different PMTs by using a 440 dichroic. The emission light going to the short wave PMT also goes through a 400/45 bandpass filter. The light to the long wave PMT goes through a 480/50 bandpass filter. Hope that helps! Paul Paul Herzmark Specialist [hidden email] Department of Molecular and Cell Biology 479 Life Science Addition University of California, Berkeley Berkeley, CA 94720-3200 (510) 643-9603 (510) 643-9500 fax On Fri, Jan 7, 2011 at 9:12 AM, Cammer, Michael <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear multiphotoners, > > I'm planning to try using Indo on the multiphoton microscope next week and > was wondering whether anybody has tips on best wavelengths to use for > excitation (e.g. 720 or 920 nm?) or anything else of potential use. > > Thank you! > > Sincerely, > > Michael > > ________________________________________________________ > Michael Cammer, Assistant Research Scientist > Skirball Institute of Biomolecular Medicine > Lab: (212) 263-3208 Cell: (914) 309-3270 > > </PRE> > <html> > <body> > ------------------------------------------------------------<br /> > This email message, including any attachments, is for the sole use of the > intended recipient(s) and may contain information that is proprietary, > confidential, and exempt from disclosure under applicable law. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > have received this email in error please notify the sender by return email > and delete the original message. Please note, the recipient should check > this email and any attachments for the presence of viruses. The organization > accepts no liability for any damage caused by any virus transmitted by this > email.<br /> > ================================= > </body> > </html> > <PRE> > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Thank you for the Indo responses! They are extremely helpful I think we're set to go. Sincerely, Michael C. ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= |
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"José A. Feijó" |
Re: Indo with multiphoton question
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In reply to this post by Paul Herzmark
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** we did it for proof of principle, fig. 28 in Feijó & Moreno, 2004, Imaging plant cells by two-photon excitation,Protoplasma, 223:1-32 (also attached) basically Paul is on spot (as usual) Em 07-01-2011 20:17, Paul Herzmark escreveu: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Michael, > > We do it here with the laser tuned to 720. We separate the two Indo peaks > into two different PMTs by using a 440 dichroic. The emission light going to > the short wave PMT also goes through a 400/45 bandpass filter. The light to > the long wave PMT goes through a 480/50 bandpass filter. > > Hope that helps! > > Paul > > > Paul Herzmark > Specialist > [hidden email] > > Department of Molecular and Cell Biology > 479 Life Science Addition > University of California, Berkeley > Berkeley, CA 94720-3200 > (510) 643-9603 > (510) 643-9500 fax > > > On Fri, Jan 7, 2011 at 9:12 AM, Cammer, Michael > <[hidden email]>wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Dear multiphotoners, >> >> I'm planning to try using Indo on the multiphoton microscope next week and >> was wondering whether anybody has tips on best wavelengths to use for >> excitation (e.g. 720 or 920 nm?) or anything else of potential use. >> >> Thank you! >> >> Sincerely, >> >> Michael >> >> ________________________________________________________ >> Michael Cammer, Assistant Research Scientist >> Skirball Institute of Biomolecular Medicine >> Lab: (212) 263-3208 Cell: (914) 309-3270 >> >> </PRE> >> <html> >> <body> >> ------------------------------------------------------------<br /> >> This email message, including any attachments, is for the sole use of the >> intended recipient(s) and may contain information that is proprietary, >> confidential, and exempt from disclosure under applicable law. Any >> unauthorized review, use, disclosure, or distribution is prohibited. If you >> have received this email in error please notify the sender by return email >> and delete the original message. Please note, the recipient should check >> this email and any attachments for the presence of viruses. The organization >> accepts no liability for any damage caused by any virus transmitted by this >> email.<br /> >> ================================= >> </body> >> </html> >> <PRE> >> > . > -- ********************************************************** Jose' A. Feijo', Prof. ---------------------------------------------------------- Dep. Biologia Vegetal, Fac.Ciencias, Universidade Lisboa PT-1749-016 Lisboa, PORTUGAL tel. +351.21.750.00.47/00/24, fax +351.21.750.00.48 and/ e Inst.Gulbenkian Ciencia, PT-2780-156 Oeiras, PORTUGAL tel. +351.21.440.79.41/00/19, fax +351.21.440.79.70 __________________________________________________________ e.mail: [hidden email] or [hidden email] URL: http://www.igc.gulbenkian.pt/research/unit/38 or http://dbv.fc.ul.pt/users/jafeijo ********************************************************** |
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Mark Cannell |
Re: Indo with multiphoton question
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In reply to this post by mcammer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We used indo-1 some time ago with some success. The details are here: Jones, K.T., Soeller, C. & Cannell, M.B. (1998) The passage of Ca2+ and fluorescent markers between the sperm and egg after fusion in the mouse. Development 125, 4627-4635. We used 690 nm but I think a bit longer would work OK too. We also used 850 nm for Ca-green. Hope this helps, Mark On 8/01/2011, at 6:12 AM, Cammer, Michael wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear multiphotoners, > > I'm planning to try using Indo on the multiphoton microscope next > week and was wondering whether anybody has tips on best wavelengths > to use for excitation (e.g. 720 or 920 nm?) or anything else of > potential use. > > Thank you! > > Sincerely, > > Michael > > ________________________________________________________ > Michael Cammer, Assistant Research Scientist > Skirball Institute of Biomolecular Medicine > Lab: (212) 263-3208 Cell: (914) 309-3270 > > </PRE> > <html> > <body> > ------------------------------------------------------------<br /> > This email message, including any attachments, is for the sole use > of the intended recipient(s) and may contain information that is > proprietary, confidential, and exempt from disclosure under > applicable law. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you have received this email in error > please notify the sender by return email and delete the original > message. Please note, the recipient should check this email and any > attachments for the presence of viruses. The organization accepts no > liability for any damage caused by any virus transmitted by this > email.<br /> > ================================= > </body> > </html> > <PRE> |
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Alberto Diaspro |
Re: Indo with multiphoton question
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** we used 720 Alby Il giorno 30/mar/2011, alle ore 21.56, Mark Cannell ha scritto: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > We used indo-1 some time ago with some success. The details are here: > > Jones, K.T., Soeller, C. & Cannell, M.B. (1998) The passage of Ca2+ and fluorescent markers between the sperm and egg after fusion in the mouse. Development 125, 4627-4635. > > > > We used 690 nm but I think a bit longer would work OK too. We also used 850 nm for Ca-green. > > > > Hope this helps, > > > > Mark > > On 8/01/2011, at 6:12 AM, Cammer, Michael wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Dear multiphotoners, >> >> I'm planning to try using Indo on the multiphoton microscope next week and was wondering whether anybody has tips on best wavelengths to use for excitation (e.g. 720 or 920 nm?) or anything else of potential use. >> >> Thank you! >> >> Sincerely, >> >> Michael >> >> ________________________________________________________ >> Michael Cammer, Assistant Research Scientist >> Skirball Institute of Biomolecular Medicine >> Lab: (212) 263-3208 Cell: (914) 309-3270 >> >> </PRE> >> <html> >> <body> >> ------------------------------------------------------------<br /> >> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> >> ================================= >> </body> >> </html> >> <PRE> ISTITUTO ITALIANO DI TECNOLOGIA Prof. Alberto Diaspro Scientific Head Nanophysics Via Morego, 30 16163 Genova Tel: +39-010.71.781.503 Fax +39-010-72.03.21 Mobile +39-3666719968 www.iit.it [hidden email] |
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Roland Nitschke |
Re: Indo with multiphoton question
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In reply to this post by mcammer
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hallo Michael, long time ago I used Indo as well as Fura-2 (right side of isobestic point excitation) with 780 nm with quite good response to Ca. Best regards Roland ___________________________ Nitschke, Roland Dr. Life Imaging Center Centre of Systems Biology Albert-Ludwigs-University Freiburg Habsburgerstr.49 D-79104 Freiburg Germany ___________________________ E-mail: [hidden email] phone: 49-761-2032934 or 2902 fax: 49-761-2032941 http://www.imaging.uni-freiburg.de/ Am 07.01.2011 18:12, schrieb Cammer, Michael: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dear multiphotoners, > > I'm planning to try using Indo on the multiphoton microscope next week and was wondering whether anybody has tips on best wavelengths to use for excitation (e.g. 720 or 920 nm?) or anything else of potential use. > > Thank you! > > Sincerely, > > Michael > > ________________________________________________________ > Michael Cammer, Assistant Research Scientist > Skirball Institute of Biomolecular Medicine > Lab: (212) 263-3208 Cell: (914) 309-3270 > > </PRE> > <html> > <body> > ------------------------------------------------------------<br /> > This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> > ================================= > </body> > </html> > <PRE> > |
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Mark Cannell-2 |
Re: Indo with multiphoton question
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** We went to 690nm to get a good info-1 ratio signal (Jones KT, Soeller C, Cannell MB. The passage of Ca2+ and fluorescent markers between the sperm and egg after fusion in the mouse. Development. 1998;125(23):4627–35. ) but tuning there may be a problem. I'd say err on the short side… Cheers Mark On 3/12/2012, at 3:05 PM, Roland Nitschke <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hallo Michael, > > long time ago I used Indo as well as Fura-2 (right side of isobestic point excitation) with 780 nm with quite good response to Ca. > > Best regards > > Roland > > ___________________________ > Nitschke, Roland Dr. > Life Imaging Center > Centre of Systems Biology > Albert-Ludwigs-University Freiburg > Habsburgerstr.49 > D-79104 Freiburg > Germany > ___________________________ > E-mail: [hidden email] > phone: 49-761-2032934 or 2902 > fax: 49-761-2032941 > http://www.imaging.uni-freiburg.de/ > > Am 07.01.2011 18:12, schrieb Cammer, Michael: >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Dear multiphotoners, >> >> I'm planning to try using Indo on the multiphoton microscope next week and was wondering whether anybody has tips on best wavelengths to use for excitation (e.g. 720 or 920 nm?) or anything else of potential use. >> >> Thank you! >> >> Sincerely, >> >> Michael >> >> ________________________________________________________ >> Michael Cammer, Assistant Research Scientist >> Skirball Institute of Biomolecular Medicine >> Lab: (212) 263-3208 Cell: (914) 309-3270 >> >> </PRE> >> <html> >> <body> >> ------------------------------------------------------------<br /> >> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.<br /> >> ================================= >> </body> >> </html> >> <PRE> >> Mark B. Cannell Ph.D. FRSNZ Professor of Cardiac Cell Biology School of Physiology& Pharmacology Medical Sciences Building University of Bristol Bristol BS8 1TD UK [hidden email] |
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