Upgrading to Win 10

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ramachan ramachan
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Upgrading to Win 10

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Dear All,

I'm sure more of you have faced the same situation and some have solved it already - the upgrade to Win 10 at microscope computers. Our University IT policy will not allow any machines running Win 7 connect to the University network after the end of Win 7 support in January 2020, which makes it a pretty urgent issue to tackle.

We have confocals from Zeiss and Leica. I've asked the vendors about the upgrade and they claim that upgrading the current workstations will result in instability. Their standard procedure would be to sell us new workstations, for which we have no budget. So the options left to us are:

EITHER upgrade the current workstations to Win 10 ourselves - this makes sense only if the instability the vendors talk about means that the configuration hasn't been verified to be stable (but might as well work perfectly fine). Any experience with this strategy?

OR to find a way how to disconnect the workstations from the University network without losing the possibility to transfer data via the network (hide them behind a NAS?) and keep the Win 7. Anyone can suggest a good solution of this type?

I hope some of you can share your experience with tackling the same issue and help us figure out what strategy will work best.

Thanks!
Radek

Radek MACHAN, Ph.D. (Senior Research Fellow)
SCELSE Advanced Biofilm Imaging Facility<http://www.scelse.sg/Page/imaging-facility> Manager
Nanyang Technological University
#B1, 60 Nanyang Drive, SBS-01N-27
Singapore 637551
Cammer, Michael Cammer, Michael
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Re: Upgrading to Win 10

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When this happened with XP to Win7, one of my colleagues installed Win7 on a computer with two Internet ports so that users could dump their confocal files to it and then transfer the files on to the school's system.  Perhaps more savvy computer people could manage this as a pass though directly, but our users had to save directly to the intermediate computer or transfer files twice.


Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016

[hidden email]<mailto:[hidden email]>  http://nyulmc.org/micros  http://microscopynotes.com/

Voice direct only, no text or messages:  1-914-309-3270 and 1-646-501-0567



________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Radek Machan (Dr) <[hidden email]>
Sent: Wednesday, November 20, 2019 8:55:47 PM
To: [hidden email]
Subject: Upgrading to Win 10

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Dear All,

I'm sure more of you have faced the same situation and some have solved it already - the upgrade to Win 10 at microscope computers. Our University IT policy will not allow any machines running Win 7 connect to the University network after the end of Win 7 support in January 2020, which makes it a pretty urgent issue to tackle.

We have confocals from Zeiss and Leica. I've asked the vendors about the upgrade and they claim that upgrading the current workstations will result in instability. Their standard procedure would be to sell us new workstations, for which we have no budget. So the options left to us are:

EITHER upgrade the current workstations to Win 10 ourselves - this makes sense only if the instability the vendors talk about means that the configuration hasn't been verified to be stable (but might as well work perfectly fine). Any experience with this strategy?

OR to find a way how to disconnect the workstations from the University network without losing the possibility to transfer data via the network (hide them behind a NAS?) and keep the Win 7. Anyone can suggest a good solution of this type?

I hope some of you can share your experience with tackling the same issue and help us figure out what strategy will work best.

Thanks!
Radek

Radek MACHAN, Ph.D. (Senior Research Fellow)
SCELSE Advanced Biofilm Imaging Facility<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.scelse.sg_Page_imaging-2Dfacility&d=DwIFAw&c=j5oPpO0eBH1iio48DtsedeElZfc04rx3ExJHeIIZuCs&r=E0xNnPAQpUbDiPlC50tp7rW2nBkvV7fujQf0RknE5bU&m=DmHBZCWHeA8r4dkXzgDeni24cldiJGyIUNEJI6evM8U&s=OCjrUbQvS-SBca_tos4b1jYXtU_sBrNTeCC7Wj0C54A&e= > Manager
Nanyang Technological University
#B1, 60 Nanyang Drive, SBS-01N-27
Singapore 637551
Rosemary White Rosemary White
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Re: Upgrading to Win 10

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You may find that your software will run on Win 10 on the updated workstations. This was certainly the case with our light microscope software from both Leica and Zeiss. We tested an install on a Win 10 workstation, hooked everything up and so far, no problems.
Confocal software may be a different story...

good luck!
cheers,
Rosemary

Dr Rosemary White
CSIRO Black Mountain
GPO Box 1700
ACT 2601, Australia

M: 61-0468966713
E: [hidden email]
________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Radek Machan (Dr) [[hidden email]]
Sent: Thursday, 21 November 2019 12:55 PM
To: [hidden email]
Subject: Upgrading to Win 10

*****
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Post images on http://www.imgur.com and include the link in your posting.
*****

Dear All,

I'm sure more of you have faced the same situation and some have solved it already - the upgrade to Win 10 at microscope computers. Our University IT policy will not allow any machines running Win 7 connect to the University network after the end of Win 7 support in January 2020, which makes it a pretty urgent issue to tackle.

We have confocals from Zeiss and Leica. I've asked the vendors about the upgrade and they claim that upgrading the current workstations will result in instability. Their standard procedure would be to sell us new workstations, for which we have no budget. So the options left to us are:

EITHER upgrade the current workstations to Win 10 ourselves - this makes sense only if the instability the vendors talk about means that the configuration hasn't been verified to be stable (but might as well work perfectly fine). Any experience with this strategy?

OR to find a way how to disconnect the workstations from the University network without losing the possibility to transfer data via the network (hide them behind a NAS?) and keep the Win 7. Anyone can suggest a good solution of this type?

I hope some of you can share your experience with tackling the same issue and help us figure out what strategy will work best.

Thanks!
Radek

Radek MACHAN, Ph.D. (Senior Research Fellow)
SCELSE Advanced Biofilm Imaging Facility<http://www.scelse.sg/Page/imaging-facility> Manager
Nanyang Technological University
#B1, 60 Nanyang Drive, SBS-01N-27
Singapore 637551
Piter_ Piter_
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Re: Upgrading to Win 10

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Hi.
If you really want to update then get a spare HDD, and install the new
system on it,
If you setup a dual boot system then you can run old system and have some
time to test the new one without disturbing normal work.
Best.
Petro.

On Thu, Nov 21, 2019 at 3:07 AM Radek Machan (Dr) <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> I'm sure more of you have faced the same situation and some have solved it
> already - the upgrade to Win 10 at microscope computers. Our University IT
> policy will not allow any machines running Win 7 connect to the University
> network after the end of Win 7 support in January 2020, which makes it a
> pretty urgent issue to tackle.
>
> We have confocals from Zeiss and Leica. I've asked the vendors about the
> upgrade and they claim that upgrading the current workstations will result
> in instability. Their standard procedure would be to sell us new
> workstations, for which we have no budget. So the options left to us are:
>
> EITHER upgrade the current workstations to Win 10 ourselves - this makes
> sense only if the instability the vendors talk about means that the
> configuration hasn't been verified to be stable (but might as well work
> perfectly fine). Any experience with this strategy?
>
> OR to find a way how to disconnect the workstations from the University
> network without losing the possibility to transfer data via the network
> (hide them behind a NAS?) and keep the Win 7. Anyone can suggest a good
> solution of this type?
>
> I hope some of you can share your experience with tackling the same issue
> and help us figure out what strategy will work best.
>
> Thanks!
> Radek
>
> Radek MACHAN, Ph.D. (Senior Research Fellow)
> SCELSE Advanced Biofilm Imaging Facility<
> http://www.scelse.sg/Page/imaging-facility> Manager
> Nanyang Technological University
> #B1, 60 Nanyang Drive, SBS-01N-27
> Singapore 637551
>
daj1u06 daj1u06
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Re: Upgrading to Win 10

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Whilst future proofing is definitely the ideal, long term solution, till you can raise the funds what you could possibly do is have the PC on the netork but off the domain, so that institution IT can't fiddle with it, then can log into domain to access filestores etc (+/- a VPN connection as required). We keep most of our microscope PC's off domain, so users log into PC with a generic, local machine login and then login to the domain to transfer files at the end of a session. Third party windows anti virus software will likely be supported for much, much longer - for example AVG still supplies winXP virus definitions! So you can reasonably self-secure an off domain PC.

In worst case scenario, take data off on memory sticks that have to be prescanned on a win 10 machine before each use.

You can also get network switches with built in firewalls to help secure you from the wider network.

The issue with trying existing software on win10 on older PC hardware could be both driver compatibility and manufacturer support.

There is an arguement that if it ain't broke, don't fix it (one of our TEM's is still running win 2000 as upgrade to win7 would have been about £20,000!) so guess it can also depend on how long you see your facility running that scope before replacing it.

Regards,

Dave Johnston,
Biomedcal Imaging Unit, University of Southampton.
Mark Cannell-2 Mark Cannell-2
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Re: Upgrading to Win 10

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How about running a windows 7 virtual machine inside windows 10?

Mark B. Cannell. Ph.D. FRSNZ FISHR
Department of Physiology, Pharmacology & Neuroscience
School of Medical Sciences
University Walk
Bristol BS8 1TD
 
[hidden email]
 
 

On 21/11/19, 8:21 AM, "Confocal Microscopy List on behalf of Dave Johnston" <[hidden email] on behalf of [hidden email]> wrote:

    *****
    To join, leave or search the confocal microscopy listserv, go to:
    http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
    Post images on http://www.imgur.com and include the link in your posting.
    *****
   
    Whilst future proofing is definitely the ideal, long term solution, till you can raise the funds what you could possibly do is have the PC on the netork but off the domain, so that institution IT can't fiddle with it, then can log into domain to access filestores etc (+/- a VPN connection as required). We keep most of our microscope PC's off domain, so users log into PC with a generic, local machine login and then login to the domain to transfer files at the end of a session. Third party windows anti virus software will likely be supported for much, much longer - for example AVG still supplies winXP virus definitions! So you can reasonably self-secure an off domain PC.
   
    In worst case scenario, take data off on memory sticks that have to be prescanned on a win 10 machine before each use.
   
    You can also get network switches with built in firewalls to help secure you from the wider network.
   
    The issue with trying existing software on win10 on older PC hardware could be both driver compatibility and manufacturer support.
   
    There is an arguement that if it ain't broke, don't fix it (one of our TEM's is still running win 2000 as upgrade to win7 would have been about £20,000!) so guess it can also depend on how long you see your facility running that scope before replacing it.
   
    Regards,
   
    Dave Johnston,
    Biomedcal Imaging Unit, University of Southampton.
   

Steve Ruzin-2 Steve Ruzin-2
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Re: Upgrading to Win 10

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*****
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*****

I've done this on two of my ZEN systems and have a couple of comments:

1) CZ will definitely sell you the upgrade, but you'll get a $2500PC
running a 2016 version of Win10 and they'll charge you at least $7500.
In the end it will work because a CZ technician will come to your lab
and do the install. Be aware that there will be NO new monitor with this
upgrade, and at least for me the video card (P400) that was included in
the new PC did not drive my 2013 HP monitor. I had to buy a new HD
monitor and video card (about $700). This is a point of contention for me.

2) If you are running ZEN 2012 or newer, and you have the ZEN install
DVD you certainly should try yourself. I did this on a second PC and
after fighting Windows for a few days it came out just fine. (I had to
REFRESH Win10)

3) If you decide to do the upgrade yourself you will need to save the
license file (in hidden Program Data:CarlZeiss:License) as well as your
particular instrument database file. Both of these very important files
will be on the CZ Flash Drive you got with your instrument (look inside
the PC side case).

4) Sometimes Windows fails cryptically during the upgrade (big
surprise). In this case you'll have to do either a "REFRESH" or Clean
Install. In both cases all your non-Windows apps will be deleted
(including ZEN). If this happens to you I would further the deletion by
UNINSTALLING ZEN once your Win10 comes back. Of course, in this case you
will have to reinstall ZEN from your ZEN >2012 DVD.

5) Finally, just make sure the PC you are upgrading will be OK with
WIn10. But my guess is that if it's newer than 6 years old it'll be OK.

Steve

Radek Machan (Dr) wrote on 11/20/19 5:55 PM:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> I'm sure more of you have faced the same situation and some have solved it already - the upgrade to Win 10 at microscope computers. Our University IT policy will not allow any machines running Win 7 connect to the University network after the end of Win 7 support in January 2020, which makes it a pretty urgent issue to tackle.
>
>
> We have confocals from Zeiss and Leica. I've asked the vendors about the upgrade and they claim that upgrading the current workstations will result in instability. Their standard procedure would be to sell us new workstations, for which we have no budget. So the options left to us are:
>
>
> EITHER upgrade the current workstations to Win 10 ourselves - this makes sense only if the instability the vendors talk about means that the configuration hasn't been verified to be stable (but might as well work perfectly fine). Any experience with this strategy?
>
>
> OR to find a way how to disconnect the workstations from the University network without losing the possibility to transfer data via the network (hide them behind a NAS?) and keep the Win 7. Anyone can suggest a good solution of this type?
>
>
> I hope some of you can share your experience with tackling the same issue and help us figure out what strategy will work best.
>
>
> Thanks!
> Radek
>
> Radek MACHAN, Ph.D. (Senior Research Fellow)
> SCELSE Advanced Biofilm Imaging Facility<http://www.scelse.sg/Page/imaging-facility> Manager
>
> Nanyang Technological University
> #B1, 60 Nanyang Drive, SBS-01N-27
> Singapore 637551

--
Steven E Ruzin, PhD
Director, Biological Imaging Facility
Curator of the Golub Collection
381 Koshland Hall
University of California
Berkeley, CA 94720-3102
http://microscopy.berkeley.edu
http://golubcollection.berkeley.edu
510-642-6602
Symeonides, Menelaos Symeonides, Menelaos
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Re: Upgrading to Win 10

In reply to this post by ramachan
*****
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*****

Want to add to this strategy something that should always be done whenever you are trying out any major software changes to a critical computer, which is to image the whole drive:

1. Create a bootable Ubuntu Live USB (version 18.04.3 LTS is good, they have instructions for how to make the USB), boot into it, select "Try Ubuntu"

2. Connect an external USB drive that is bigger than the capacity of your OS drive (the file size of the image will be the capacity of the drive, not how much data is actually on it)

3. Click "Show Applications" on the bottom left and select "Disks"

4. Select your OS drive in the list on the left

5. Click on each partition and make sure it is unmounted, if it got automatically mounted then click the "stop" icon to unmount it

6. Click the menu icon (three horizontal lines) on the top right of the window and select "Create Disk Image"

7. Set your external USB drive next to "Save in Folder" and click "Start Creating"

This will create a 100% accurate sector-by-sector copy of the drive, including all partitions (hidden or not, recovery partitions, boot sector, everything), that can later be restored in case things go wrong during your upgrade and cannot be salvaged. Just repeat the above process and instead select "Restore Disk Image", and point to the image file you created on your external USB drive. This will completely erase everything currently on the drive and restore the exact state at which the OS drive was previously imaged. Note that if you make any hardware changes and then restore a drive image that was created before the hardware changes, this may confuse Windows somewhat depending on how extensive the changes were.

I also agree that if the computer is currently running Windows 7 and is relatively recently built, a Windows 10 upgrade is definitely worth trying. Some of the nicer companies out there may even help you tweak things to full functionality in case the connected instrument doesn't work perfectly right off the bat (which might be e.g. just a new driver or a setting change).

Good luck!
Mel

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Steven Ruzin
Sent: Thursday, November 21, 2019 10:27 AM
To: [hidden email]
Subject: Re: Upgrading to Win 10

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I've done this on two of my ZEN systems and have a couple of comments:

1) CZ will definitely sell you the upgrade, but you'll get a $2500PC running a 2016 version of Win10 and they'll charge you at least $7500.
In the end it will work because a CZ technician will come to your lab and do the install. Be aware that there will be NO new monitor with this upgrade, and at least for me the video card (P400) that was included in the new PC did not drive my 2013 HP monitor. I had to buy a new HD monitor and video card (about $700). This is a point of contention for me.

2) If you are running ZEN 2012 or newer, and you have the ZEN install DVD you certainly should try yourself. I did this on a second PC and after fighting Windows for a few days it came out just fine. (I had to REFRESH Win10)

3) If you decide to do the upgrade yourself you will need to save the license file (in hidden Program Data:CarlZeiss:License) as well as your particular instrument database file. Both of these very important files will be on the CZ Flash Drive you got with your instrument (look inside the PC side case).

4) Sometimes Windows fails cryptically during the upgrade (big surprise). In this case you'll have to do either a "REFRESH" or Clean Install. In both cases all your non-Windows apps will be deleted (including ZEN). If this happens to you I would further the deletion by UNINSTALLING ZEN once your Win10 comes back. Of course, in this case you will have to reinstall ZEN from your ZEN >2012 DVD.

5) Finally, just make sure the PC you are upgrading will be OK with WIn10. But my guess is that if it's newer than 6 years old it'll be OK.

Steve

Radek Machan (Dr) wrote on 11/20/19 5:55 PM:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> I'm sure more of you have faced the same situation and some have solved it already - the upgrade to Win 10 at microscope computers. Our University IT policy will not allow any machines running Win 7 connect to the University network after the end of Win 7 support in January 2020, which makes it a pretty urgent issue to tackle.
>
>
> We have confocals from Zeiss and Leica. I've asked the vendors about the upgrade and they claim that upgrading the current workstations will result in instability. Their standard procedure would be to sell us new workstations, for which we have no budget. So the options left to us are:
>
>
> EITHER upgrade the current workstations to Win 10 ourselves - this makes sense only if the instability the vendors talk about means that the configuration hasn't been verified to be stable (but might as well work perfectly fine). Any experience with this strategy?
>
>
> OR to find a way how to disconnect the workstations from the University network without losing the possibility to transfer data via the network (hide them behind a NAS?) and keep the Win 7. Anyone can suggest a good solution of this type?
>
>
> I hope some of you can share your experience with tackling the same issue and help us figure out what strategy will work best.
>
>
> Thanks!
> Radek
>
> Radek MACHAN, Ph.D. (Senior Research Fellow) SCELSE Advanced Biofilm 
> Imaging Facility<http://www.scelse.sg/Page/imaging-facility> Manager
>
> Nanyang Technological University
> #B1, 60 Nanyang Drive, SBS-01N-27
> Singapore 637551

--
Steven E Ruzin, PhD
Director, Biological Imaging Facility
Curator of the Golub Collection
381 Koshland Hall
University of California
Berkeley, CA 94720-3102
http://microscopy.berkeley.edu
http://golubcollection.berkeley.edu
510-642-6602
Stanislav Vitha-2 Stanislav Vitha-2
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Re: Upgrading to Win 10

In reply to this post by ramachan
*****
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Post images on http://www.imgur.com and include the link in your posting.
*****

We use the same approach as Michael described, on our EM and LM instruments with old systems (some still running XP after being upgraded from Win 2000). The microscope PC is offline, connected with an Etherent cable to a support PC running the latest system. The support PC has a second network card which is connected to the internet and has automatic system and antivirus updates.
Users should be prevented from plugging their portable storage directly into the microscope PC.

Users acquire data on the microscope PC, then copy their files to the support PC that shows up as a networked drive on the desktop.   Users then can log into the support PC using the standard university-wide authentication, and copy their files to a portable drive.

This has the advantage that 1) we do not have to manage the support PC (the IT department does) and 2) the support PC does not have to be in the same room as the microscope. For instance, one of our confocals is in a BL-2 lab, and the support PC that we are about to install will be in a common computer room in the facility. Since the standard login procedure into a network-connected computer  requires a cell phone confirmation,  it is better to do this outside a BL-2 lab, rather than having to surface-sterilize your phone when it gets touched with gloved hands.    


Stan Vitha
Microscopy and Imaging Center
Texas A&M University
http://microscopy.tamu.edu


On Thu, 21 Nov 2019 02:46:23 +0000, Cammer, Michael <[hidden email]> wrote:
>When this happened with XP to Win7, one of my colleagues installed Win7 on a computer with two Internet ports so that users could dump their confocal files to it and then transfer the files on to the school's system.  Perhaps more savvy computer people could manage this as a pass though directly, but our users had to save directly to the intermediate computer or transfer files twice.
>
>
>Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>
Craig Brideau Craig Brideau
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Re: Upgrading to Win 10

In reply to this post by Symeonides, Menelaos
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Our IT department created a restricted subnet for all our 'legacy' systems.
Basically they closed off all the ports and access to these machines except
the bare minimum we need to operate them. Not ideal, but it works. In terms
of trying to upgrade yourself, follow the advise of the listers that
suggest creating a disk image of the original state of your drive before
tinkering with upgrading. This will give you a safe way to revert if all
goes wrong. Make sure whatever device you use to store the image is highly
reliable!

Craig

On Thu, Nov 21, 2019 at 9:48 AM Symeonides, Menelaos <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Want to add to this strategy something that should always be done whenever
> you are trying out any major software changes to a critical computer, which
> is to image the whole drive:
>
> 1. Create a bootable Ubuntu Live USB (version 18.04.3 LTS is good, they
> have instructions for how to make the USB), boot into it, select "Try
> Ubuntu"
>
> 2. Connect an external USB drive that is bigger than the capacity of your
> OS drive (the file size of the image will be the capacity of the drive, not
> how much data is actually on it)
>
> 3. Click "Show Applications" on the bottom left and select "Disks"
>
> 4. Select your OS drive in the list on the left
>
> 5. Click on each partition and make sure it is unmounted, if it got
> automatically mounted then click the "stop" icon to unmount it
>
> 6. Click the menu icon (three horizontal lines) on the top right of the
> window and select "Create Disk Image"
>
> 7. Set your external USB drive next to "Save in Folder" and click "Start
> Creating"
>
> This will create a 100% accurate sector-by-sector copy of the drive,
> including all partitions (hidden or not, recovery partitions, boot sector,
> everything), that can later be restored in case things go wrong during your
> upgrade and cannot be salvaged. Just repeat the above process and instead
> select "Restore Disk Image", and point to the image file you created on
> your external USB drive. This will completely erase everything currently on
> the drive and restore the exact state at which the OS drive was previously
> imaged. Note that if you make any hardware changes and then restore a drive
> image that was created before the hardware changes, this may confuse
> Windows somewhat depending on how extensive the changes were.
>
> I also agree that if the computer is currently running Windows 7 and is
> relatively recently built, a Windows 10 upgrade is definitely worth trying.
> Some of the nicer companies out there may even help you tweak things to
> full functionality in case the connected instrument doesn't work perfectly
> right off the bat (which might be e.g. just a new driver or a setting
> change).
>
> Good luck!
> Mel
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Steven Ruzin
> Sent: Thursday, November 21, 2019 10:27 AM
> To: [hidden email]
> Subject: Re: Upgrading to Win 10
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I've done this on two of my ZEN systems and have a couple of comments:
>
> 1) CZ will definitely sell you the upgrade, but you'll get a $2500PC
> running a 2016 version of Win10 and they'll charge you at least $7500.
> In the end it will work because a CZ technician will come to your lab and
> do the install. Be aware that there will be NO new monitor with this
> upgrade, and at least for me the video card (P400) that was included in the
> new PC did not drive my 2013 HP monitor. I had to buy a new HD
> monitor and video card (about $700). This is a point of contention for me.
>
> 2) If you are running ZEN 2012 or newer, and you have the ZEN install DVD
> you certainly should try yourself. I did this on a second PC and after
> fighting Windows for a few days it came out just fine. (I had to
> REFRESH Win10)
>
> 3) If you decide to do the upgrade yourself you will need to save the
> license file (in hidden Program Data:CarlZeiss:License) as well as your
> particular instrument database file. Both of these very important files
> will be on the CZ Flash Drive you got with your instrument (look inside
> the PC side case).
>
> 4) Sometimes Windows fails cryptically during the upgrade (big surprise).
> In this case you'll have to do either a "REFRESH" or Clean Install. In both
> cases all your non-Windows apps will be deleted (including ZEN). If this
> happens to you I would further the deletion by UNINSTALLING ZEN once your
> Win10 comes back. Of course, in this case you
> will have to reinstall ZEN from your ZEN >2012 DVD.
>
> 5) Finally, just make sure the PC you are upgrading will be OK with
> WIn10. But my guess is that if it's newer than 6 years old it'll be OK.
>
> Steve
>
> Radek Machan (Dr) wrote on 11/20/19 5:55 PM:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com
>  and include the link in your posting.
> > *****
> >
> > Dear All,
> >
> >
> I'm sure more of you have faced the same situation and some have solved it already - the upgrade to Win 10 at microscope computers. Our University IT policy will not allow any machines running Win 7 connect to the University network after the end of Win 7 support in January 2020, which makes it a pretty urgent issue to tackle.
>
> >
> >
> >
> We have confocals from Zeiss and Leica. I've asked the vendors about the upgrade and they claim that upgrading the current workstations will result in instability. Their standard procedure would be to sell us new workstations, for which we have no budget. So the options left to us are:
>
> >
> >
> >
> EITHER upgrade the current workstations to Win 10 ourselves - this makes sense only if the instability the vendors talk about means that the configuration hasn't been verified to be stable (but might as well work perfectly fine). Any experience with this strategy?
>
> >
> >
> >
> OR to find a way how to disconnect the workstations from the University network without losing the possibility to transfer data via the network (hide them behind a NAS?) and keep the Win 7. Anyone can suggest a good solution of this type?
>
> >
> >
> >
> I hope some of you can share your experience with tackling the same issue and help us figure out what strategy will work best.
>
> >
> >
> > Thanks!
> > Radek
> >
> > Radek MACHAN, Ph.D. (Senior Research Fellow) SCELSE Advanced Biofilm
> > Imaging Facility<http://www.scelse.sg/Page/imaging-facility> Manager
> >
> > Nanyang Technological University
> > #B1, 60 Nanyang Drive, SBS-01N-27
> > Singapore 637551
>
> --
> Steven E Ruzin, PhD
> Director, Biological Imaging Facility
> Curator of the Golub Collection
> 381 Koshland Hall
> University of California
> Berkeley, CA 94720-3102
> http://microscopy.berkeley.edu
> http://golubcollection.berkeley.edu
> 510-642-6602
>
Mark Scott-3 Mark Scott-3
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Re: Upgrading to Win 10

In reply to this post by ramachan
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*****

Dear Radek,

It is a problem where unfortunately a blanket IT ban on those computers isn't really a helpful solution.  While we can all debate the why's and wherefores of vendors not having had their software moved to Win10 by now, but the reality is that some of these systems will not be moved over in time, if at all.  IT, while I understand their security concerns and general policies, having no backup plan isn't really helping people who have no choice but to run outdated Oss.

While some vendors are actually working on testing Win10 rollouts for their acquisition software, these are unlikely to be out in time for the cut-off date and others still just won't be investing money into developing software for outdated platforms anyway (and the size of some companies I think it is entirely justified as well).

I actually spoke to our IT people and explained the situation, and said that if they blanket ban systems that are still on Win7/XP then we will have a lot of very expensive equipment sitting idle being unable to be used and a lot of very irate researchers who won't be able to do their experiments.  This seemed to have a general softening of their stance and a solution was then found to isolate those computers that would still need to be on Win7/XP and place them on their own little firewalled secure island within the network, (something that has tended to be the norm anyway at some institutes I've worked at in the past), while allowing specific web-addresses and internal network locations to be white-listed for access.

Simply requesting everyone to upgrade to Win10 isn't the answer, unless IT are willing to offer financial assistance to help with the upgrades.  Often what we have found is an upgrade of the PC of said microscope will be excessively expensive, which often then also means the camera adaptors or some other hardware interface no longer works and these need to be upgraded as well.  It isn't as simple as just installing Win10 on a system because as we all know from the Leica issues recently, this hasn't been tested with the microscope software/hardware and the vendors will not support it if it doesn't work.

In my experience, you need to explain the reasons why, the financial and support implications of upgrading and the effect it has on the end user/researcher and the potential lost revenue/wasted money.  Other than that, ask IT to create a networked PC that you can use as a file dump that IS running Win10, then use that as your intermediary computer.

Good Luck
Mark


-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Radek Machan (Dr)
Sent: Thursday, 21 November 2019 11:56 AM
To: [hidden email]
Subject: Upgrading to Win 10

This email has been identified as possessing SPAM like characteristics, please proceed with care. If you have any concerns please contact TRI ICT.
*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

Dear All,

I'm sure more of you have faced the same situation and some have solved it already - the upgrade to Win 10 at microscope computers. Our University IT policy will not allow any machines running Win 7 connect to the University network after the end of Win 7 support in January 2020, which makes it a pretty urgent issue to tackle.

We have confocals from Zeiss and Leica. I've asked the vendors about the upgrade and they claim that upgrading the current workstations will result in instability. Their standard procedure would be to sell us new workstations, for which we have no budget. So the options left to us are:

EITHER upgrade the current workstations to Win 10 ourselves - this makes sense only if the instability the vendors talk about means that the configuration hasn't been verified to be stable (but might as well work perfectly fine). Any experience with this strategy?

OR to find a way how to disconnect the workstations from the University network without losing the possibility to transfer data via the network (hide them behind a NAS?) and keep the Win 7. Anyone can suggest a good solution of this type?

I hope some of you can share your experience with tackling the same issue and help us figure out what strategy will work best.

Thanks!
Radek

Radek MACHAN, Ph.D. (Senior Research Fellow) SCELSE Advanced Biofilm Imaging Facility<http://www.scelse.sg/Page/imaging-facility> Manager Nanyang Technological University #B1, 60 Nanyang Drive, SBS-01N-27 Singapore 637551
Colin Gray Colin Gray
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Re: Upgrading to Win 10

In reply to this post by ramachan
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*****

Hi,

I had this problem with our WinXP machines and have it again now with
Win7. Fortunately my university saw sense last time and realised that it
would be a massive expense (money we do not have) to update or replace
all of the equipment (even the worker hours required to
update/debug/fine tune all such systems across the university is enormous).

After some argument they put everyone who had complained enough onto a
separate vLAN. We have local machine based logins but once, logged in
users can easily map a network drive. This solution also supports our
PPMS user login activated real-time booking and billing system.

Being on a restricted vLAN has caused us no problems at all.

Sadly the same cannot be said for machines running Windows 10. The last
major update did not support our camera so we had to roll back. However,
then Win 10 would not let us choose which updates to install so we had
to block all updates, including security ones. Fortunately we were not
alone and the manufacturer eventually found a solution- this time!

Another problem with Windows 10 is that shutting down the computer does
not logout the user (as anyone using a login based billing system will
soon discover).

If you are still tempted to upgrade consider how much your time is worth
and the cost of system downtime while you do it. If you do the upgrades,
how will this affect your service contracts. Consider all this vs a new
computer and fully supported system. What you save in cash you can
easily pay in sweat and pain so, sometimes what looks like the expensive
option is really not so bad after all.


Good luck


Colin


--
Colin Gray  PhD

[hidden email]

Microscopy Core Facility, Room LU104.



--------------------------------------------------------------

Tel + 44 (0)114 2159580

Postal address:

Dr C Gray
Department of Infection, Immunity & Cardiovascular Disease
Faculty of Medicine, Dentistry & Health
University of Sheffield
Medical School
Beech Hill Road
Sheffield
S10 2RX
Göran Månsson Göran Månsson
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Re: Upgrading to Win 10

*****
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*****

Hi,

Regarding the problem that Windows 10 allows only for the previous user to log in after a restart, I had the same problem and solved it like this:

- Search for Edit Group Policy
- Go to Admin Templates/Windows Components/Windows Logon Options
- Disable the "Sign-in last interactive user automatically..."

Best regards
Göran

Göran Månsson, Manager
BIC – Biomedicum Imaging Core
Karolinska Institutet, Stockholm Sweden




-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Colin Gray
Sent: 22 November 2019 12:05
To: [hidden email]
Subject: Re: Upgrading to Win 10

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi,

I had this problem with our WinXP machines and have it again now with Win7. Fortunately my university saw sense last time and realised that it would be a massive expense (money we do not have) to update or replace all of the equipment (even the worker hours required to update/debug/fine tune all such systems across the university is enormous).

After some argument they put everyone who had complained enough onto a separate vLAN. We have local machine based logins but once, logged in users can easily map a network drive. This solution also supports our PPMS user login activated real-time booking and billing system.

Being on a restricted vLAN has caused us no problems at all.

Sadly the same cannot be said for machines running Windows 10. The last major update did not support our camera so we had to roll back. However, then Win 10 would not let us choose which updates to install so we had to block all updates, including security ones. Fortunately we were not alone and the manufacturer eventually found a solution- this time!

Another problem with Windows 10 is that shutting down the computer does not logout the user (as anyone using a login based billing system will soon discover).

If you are still tempted to upgrade consider how much your time is worth and the cost of system downtime while you do it. If you do the upgrades, how will this affect your service contracts. Consider all this vs a new computer and fully supported system. What you save in cash you can easily pay in sweat and pain so, sometimes what looks like the expensive option is really not so bad after all.


Good luck


Colin


--
Colin Gray  PhD

[hidden email]

Microscopy Core Facility, Room LU104.



--------------------------------------------------------------

Tel + 44 (0)114 2159580

Postal address:

Dr C Gray
Department of Infection, Immunity & Cardiovascular Disease Faculty of Medicine, Dentistry & Health University of Sheffield Medical School Beech Hill Road Sheffield
S10 2RX
Steffen Dietzel Steffen Dietzel
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Re: Upgrading to Win 10

In reply to this post by ramachan
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*****

Hi all,

a very timely discussion, since I just started to upgrade our Leica
microscope computers from Win 7 to Win 10. I did not want to make a
clean install, to keep all the users and instrument settings.

I started with the easy ones, widefield systems with no microscope
control, just camera-connection and plan to continue to the more complex
machines until I hit problems. I decided to upgrade WF to Win 10
Enterprise LTSC 2019, I am not sure if this version is suitable for
confocals. Might only use LTSC 2016 for them, if the 2019 is not yet
supported (we will get a new SP8 system soon, then I can check what
Leica puts on it at this time.)

Anyway, I made a step-by-step protocol, mostly for myself for the next
computers to come. In case you care, you can find it here:

https://www.bioimaging.bmc.med.uni-muenchen.de/manuals-protocols/maintenance_protocolls/win7towin10/index.html

This might contain mistakes, so enter at your one risk. If you find any,
please let me know.

Best

Steffen


Am 21.11.2019 um 02:55 schrieb Radek Machan (Dr):

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> I'm sure more of you have faced the same situation and some have solved it already - the upgrade to Win 10 at microscope computers. Our University IT policy will not allow any machines running Win 7 connect to the University network after the end of Win 7 support in January 2020, which makes it a pretty urgent issue to tackle.
>
> We have confocals from Zeiss and Leica. I've asked the vendors about the upgrade and they claim that upgrading the current workstations will result in instability. Their standard procedure would be to sell us new workstations, for which we have no budget. So the options left to us are:
>
> EITHER upgrade the current workstations to Win 10 ourselves - this makes sense only if the instability the vendors talk about means that the configuration hasn't been verified to be stable (but might as well work perfectly fine). Any experience with this strategy?
>
> OR to find a way how to disconnect the workstations from the University network without losing the possibility to transfer data via the network (hide them behind a NAS?) and keep the Win 7. Anyone can suggest a good solution of this type?
>
> I hope some of you can share your experience with tackling the same issue and help us figure out what strategy will work best.
>
> Thanks!
> Radek
>
> Radek MACHAN, Ph.D. (Senior Research Fellow)
> SCELSE Advanced Biofilm Imaging Facility<http://www.scelse.sg/Page/imaging-facility> Manager
> Nanyang Technological University
> #B1, 60 Nanyang Drive, SBS-01N-27
> Singapore 637551
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de
ramachan ramachan
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Re: Upgrading to Win 10

In reply to this post by ramachan
*****
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*****

Dear All,

Thank you for your valuable inputs to this discussion, which, I'm sure, will be of substantial interest at this time.

As for us, things have resolved themselves in the way I originally hoped for. Our microscope workstations are connected to a server running OMERO via a dedicated local network and have no direct connection to the University-wide network. I had a second talk with our IT and convince him that, since we do not connect the machines directly to the University network, we are not violating the IT policies by continuing running Win 7. So to my great relief we do not need to modify what works well enough, at least not immediately under pressure of time.

Good luck to everyone in tackling this issue!
Best wishes,
Radek

Radek MACHAN, Ph.D. (Senior Research Fellow)
SCELSE Advanced Biofilm Imaging Facility<http://www.scelse.sg/Page/imaging-facility> Manager
Nanyang Technological University
#B1, 60 Nanyang Drive, SBS-01N-27
Singapore 637551
Steffen Dietzel Steffen Dietzel
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Re: Upgrading to Win 10

In reply to this post by Göran Månsson
*****
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A bit late, but I guess some are still struggling, so:

In addition to Göran's procedure to disable automatic re-logon after
booting, I found a way to disable the option to lock the screen while
still being logged in. This should disable the possibility that two
users are logged in at the same time, another problem for tracking usage
time:

Go to "Control Panel\Hardware and Sound\Power Options" and click on
"Choose what the power buttons do". Select "Change Settings that are
currently unavailable" and remove the check mark in front of "Lock".

So far I updated 5 widefield systems to Win10 LTSC 2019. It was a bit
bumpy, but it seems to have worked. Next week I will try a confocal
(SP8) with LTSB 2016, the version that Leica is currently distributing.
The PPMS tracker was not running on all machines, on others it does, I
still have to work on this.

With the long term servicing versions, windows updates should hopefully
not cause the type of problems that Colin was mentioning.

Steffen


Am 22.11.2019 um 13:40 schrieb Göran Månsson:

> Hi,
>
> Regarding the problem that Windows 10 allows only for the previous
> user to log in after a restart, I had the same problem and solved it
> like this:
>
> - Search for Edit Group Policy
> - Go to Admin Templates/Windows Components/Windows Logon Options
> - Disable the "Sign-in last interactive user automatically..."
>
> Best regards
> Göran
>
> Göran Månsson, Manager
> BIC – Biomedicum Imaging Core
> Karolinska Institutet, Stockholm Sweden
>
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On
> Behalf Of Colin Gray
> Sent: 22 November 2019 12:05
> To: [hidden email]
> Subject: Re: Upgrading to Win 10
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> I had this problem with our WinXP machines and have it again now with
> Win7. Fortunately my university saw sense last time and realised that
> it would be a massive expense (money we do not have) to update or
> replace all of the equipment (even the worker hours required to
> update/debug/fine tune all such systems across the university is
> enormous).
>
> After some argument they put everyone who had complained enough onto a
> separate vLAN. We have local machine based logins but once, logged in
> users can easily map a network drive. This solution also supports our
> PPMS user login activated real-time booking and billing system.
>
> Being on a restricted vLAN has caused us no problems at all.
>
> Sadly the same cannot be said for machines running Windows 10. The
> last major update did not support our camera so we had to roll back.
> However, then Win 10 would not let us choose which updates to install
> so we had to block all updates, including security ones. Fortunately
> we were not alone and the manufacturer eventually found a solution-
> this time!
>
> Another problem with Windows 10 is that shutting down the computer
> does not logout the user (as anyone using a login based billing system
> will soon discover).
>
> If you are still tempted to upgrade consider how much your time is
> worth and the cost of system downtime while you do it. If you do the
> upgrades, how will this affect your service contracts. Consider all
> this vs a new computer and fully supported system. What you save in
> cash you can easily pay in sweat and pain so, sometimes what looks
> like the expensive option is really not so bad after all.
>
>
> Good luck
>
>
> Colin
>
>
> --
> Colin Gray PhD
>
> [hidden email]
>
> Microscopy Core Facility, Room LU104.
>
>
>
> --------------------------------------------------------------
>
> Tel + 44 (0)114 2159580
>
> Postal address:
>
> Dr C Gray
> Department of Infection, Immunity & Cardiovascular Disease Faculty of
> Medicine, Dentistry & Health University of Sheffield Medical School
> Beech Hill Road Sheffield
> S10 2RX
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de
Steffen Dietzel Steffen Dietzel
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Re: Upgrading to Win 10 (Leica)

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Dear all,

coming back to this thread from November,  I can now report that in the
meantime I successfully updated eight Leica microscope computers from
Windows 7 to Windows 10 (Enterprise long term support), including three
SP8 systems.

While the road was bumpy, they now all run fine without problems. Once I
figured out how to do it, it was rather simple for the following
comparable systems (WF or SP8). Still time consuming though. The key to
sleeping well is to make a backup of the system disk before starting
anything, no surprise here.

Because I had that many systems to upgrade, I made a web page with the
procedure that I used as reference with the following systems. Since it
might be useful to some of you, here is the link.
https://www.bioimaging.bmc.med.uni-muenchen.de/manuals-protocols/maintenance_protocolls/win7towin10/index.html

This is not a procedure endorsed by Leica, but it worked for us. It
would be of interest to me if this is actually helpful to anybody, if so
I would appreciate a message. (I won't be able to read e-mails from this
account next week and the week thereafter, sorry, any reply will be
delayed).

Thanks to those who helped with valuable tips.

The usual disclaimer applies: No warranties. Enter at your own risk.
Make a backup first.

Best

Steffen



--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de
Arvydas Matiukas Arvydas Matiukas
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Re: [EXTERNAL] Re: Upgrading to Win 10 (Leica)

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Steffen,

Thanks a lot. This is a great recourse for do-it-yourself enthusiasts.

I wonder if you have a procedure to move/transfer confocal software
to another/new computer in case old computer (i.e. motherboard) dies.

More generally what precaution measures do you recommend to deal with
the situation (less likely then disk damage/death but still not
impossible after
>4-5 years)

Thanks,
Arvydas





+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Neuroscience & Physiology Dept


Syracuse, NY 13210


>>> Steffen Dietzel <[hidden email]> 01/28/20 7:15 AM >>>
*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=px8351lW7zoI3LDUz6p7ax5VwuWmLFD257o8bb3RxBc&e=

Post images on
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=Eij_pLUIOG-byyODG-K6bT9hbHbcwNsdQKZnHSDBtW8&e=
 and include the link in your posting.
*****

Dear all,

coming back to this thread from November,  I can now report that in the
meantime I successfully updated eight Leica microscope computers from
Windows 7 to Windows 10 (Enterprise long term support), including three
SP8 systems.

While the road was bumpy, they now all run fine without problems. Once I

figured out how to do it, it was rather simple for the following
comparable systems (WF or SP8). Still time consuming though. The key to
sleeping well is to make a backup of the system disk before starting
anything, no surprise here.

Because I had that many systems to upgrade, I made a web page with the
procedure that I used as reference with the following systems. Since it
might be useful to some of you, here is the link.
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_manuals-2Dprotocols_maintenance-5Fprotocolls_win7towin10_index.html&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=5lafTeoK-FTvGr-LVudmzLXQy3JJHEG29aWxam7JMMk&e=


This is not a procedure endorsed by Leica, but it worked for us. It
would be of interest to me if this is actually helpful to anybody, if so

I would appreciate a message. (I won't be able to read e-mails from this

account next week and the week thereafter, sorry, any reply will be
delayed).

Thanks to those who helped with valuable tips.

The usual disclaimer applies: No warranties. Enter at your own risk.
Make a backup first.

Best

Steffen



--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

https://urldefense.proofpoint.com/v2/url?u=http-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=hilDpakShU18PyEWRrkp5qQLzB1HYfr7LpY1mH6MKHU&e=
Steffen Dietzel Steffen Dietzel
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Re: [EXTERNAL] Re: Upgrading to Win 10 (Leica)

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Arvydas,

I am afraid I don't have a procedure to move to different computer
hardware. From what I've learned during the updates, I would think that
there are two indispensable pieces of hardware for the SP8: The ethernet
adapter that connects to the scanner and the bridge that connects to WLL
or MP laser. (But I may be missing something. A closer inspection of the
connections that leave the computer should tell.) If those two still
work after a computer crash, I guess it would be worth a shot if you
can't get a new computer from the manufacturer. I am not enough into
computer hardware to guess if these pieces are easy or not to replace
with equivalent hardware. Best to use a computer which also could be
used for office stuff in case the integration with the confocal does not
work out.

With the update, my advantage was that I knew the hardware was intact.
It was 'just' a configuration problem.

As precautions, having a backup is on the top of the list. Depending on
local paranoia levels, a cloned (and tested) system SSD might be
reassuring (in addition to the backup). Windows 10 has the nice property
of healing itself during boot. If you swap a system disk into a new
computer, it usually works (you might have to reactivate Windows). So if
the new computer also contains the special hardware, just maybe...

You also might want to write down the connection parameters that I
mention in the procedure.

Best

Steffen

Am 28.01.2020 um 13:55 schrieb Arvydas Matiukas:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Steffen,
>
> Thanks a lot. This is a great recourse for do-it-yourself enthusiasts.
>
> I wonder if you have a procedure to move/transfer confocal software
> to another/new computer in case old computer (i.e. motherboard) dies.
>
> More generally what precaution measures do you recommend to deal with
> the situation (less likely then disk damage/death but still not
> impossible after
>> 4-5 years)
> Thanks,
> Arvydas
>
>
>
>
>
> +++++++++++++++++++++++++++++
> Arvydas Matiukas, Ph.D.
> Manager of NRB Shared Equipment
> Director of Confocal&Two-Photon Core
> SUNY Upstate Medical University
> Neuroscience & Physiology Dept
>
>
> Syracuse, NY 13210
>
>
>>>> Steffen Dietzel <[hidden email]> 01/28/20 7:15 AM >>>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=px8351lW7zoI3LDUz6p7ax5VwuWmLFD257o8bb3RxBc&e=
>
> Post images on
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=Eij_pLUIOG-byyODG-K6bT9hbHbcwNsdQKZnHSDBtW8&e=
> and include the link in your posting.
> *****
>
> Dear all,
>
> coming back to this thread from November, I can now report that in the
> meantime I successfully updated eight Leica microscope computers from
> Windows 7 to Windows 10 (Enterprise long term support), including three
> SP8 systems.
>
> While the road was bumpy, they now all run fine without problems. Once I
>
> figured out how to do it, it was rather simple for the following
> comparable systems (WF or SP8). Still time consuming though. The key to
> sleeping well is to make a backup of the system disk before starting
> anything, no surprise here.
>
> Because I had that many systems to upgrade, I made a web page with the
> procedure that I used as reference with the following systems. Since it
> might be useful to some of you, here is the link.
> https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_manuals-2Dprotocols_maintenance-5Fprotocolls_win7towin10_index.html&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=5lafTeoK-FTvGr-LVudmzLXQy3JJHEG29aWxam7JMMk&e=
>
>
> This is not a procedure endorsed by Leica, but it worked for us. It
> would be of interest to me if this is actually helpful to anybody, if so
>
> I would appreciate a message. (I won't be able to read e-mails from this
>
> account next week and the week thereafter, sorry, any reply will be
> delayed).
>
> Thanks to those who helped with valuable tips.
>
> The usual disclaimer applies: No warranties. Enter at your own risk.
> Make a backup first.
>
> Best
>
> Steffen
>
>
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de
Lemasters, John J. Lemasters, John J.
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Re: [EXTERNAL] Re: Upgrading to Win 10 (Leica)

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Can anyone comment on upgrading to Win 10 for a Zeiss LSM 880 microscope and earlier Zeiss instruments? Sorry if I missed earlier threads.

Thanks, John

--
John J. Lemasters, MD, PhD
Professor and GlaxoSmithKline Distinguished Endowed Chair
Director, Center for Cell Death, Injury & Regeneration
Departments of Drug Discovery & Biomedical Sciences and Biochemistry & Molecular Biology
Medical University of South Carolina
DD504 Drug Discovery Building
70 President Street, MSC 139
Charleston, SC 29425

Office: 843-876-2360
Lab: 843-876-2354
Fax: 843-876-2353
Email: [hidden email]
https://education.musc.edu/MUSCApps/FacultyDirectory/Lemasters-John

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Steffen Dietzel
Sent: Tuesday, January 28, 2020 8:52 AM
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Upgrading to Win 10 (Leica)

CAUTION: External

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Arvydas,

I am afraid I don't have a procedure to move to different computer hardware. From what I've learned during the updates, I would think that there are two indispensable pieces of hardware for the SP8: The ethernet adapter that connects to the scanner and the bridge that connects to WLL or MP laser. (But I may be missing something. A closer inspection of the connections that leave the computer should tell.) If those two still work after a computer crash, I guess it would be worth a shot if you can't get a new computer from the manufacturer. I am not enough into computer hardware to guess if these pieces are easy or not to replace with equivalent hardware. Best to use a computer which also could be used for office stuff in case the integration with the confocal does not work out.

With the update, my advantage was that I knew the hardware was intact.
It was 'just' a configuration problem.

As precautions, having a backup is on the top of the list. Depending on local paranoia levels, a cloned (and tested) system SSD might be reassuring (in addition to the backup). Windows 10 has the nice property of healing itself during boot. If you swap a system disk into a new computer, it usually works (you might have to reactivate Windows). So if the new computer also contains the special hardware, just maybe...

You also might want to write down the connection parameters that I mention in the procedure.

Best

Steffen

Am 28.01.2020 um 13:55 schrieb Arvydas Matiukas:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Steffen,
>
> Thanks a lot. This is a great recourse for do-it-yourself enthusiasts.
>
> I wonder if you have a procedure to move/transfer confocal software to
> another/new computer in case old computer (i.e. motherboard) dies.
>
> More generally what precaution measures do you recommend to deal with
> the situation (less likely then disk damage/death but still not
> impossible after
>> 4-5 years)
> Thanks,
> Arvydas
>
>
>
>
>
> +++++++++++++++++++++++++++++
> Arvydas Matiukas, Ph.D.
> Manager of NRB Shared Equipment
> Director of Confocal&Two-Photon Core
> SUNY Upstate Medical University
> Neuroscience & Physiology Dept
>
>
> Syracuse, NY 13210
>
>
>>>> Steffen Dietzel <[hidden email]> 01/28/20 7:15 AM >>>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-
> 2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r
> =DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOi
> Ceg2gXbRKTMkbongtDE0&s=px8351lW7zoI3LDUz6p7ax5VwuWmLFD257o8bb3RxBc&e=
>
> Post images on
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=Dw
> IDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK
> 6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=Eij_pLUIOG-byyOD
> G-K6bT9hbHbcwNsdQKZnHSDBtW8&e=
> and include the link in your posting.
> *****
>
> Dear all,
>
> coming back to this thread from November, I can now report that in the
> meantime I successfully updated eight Leica microscope computers from
> Windows 7 to Windows 10 (Enterprise long term support), including
> three
> SP8 systems.
>
> While the road was bumpy, they now all run fine without problems. Once
> I
>
> figured out how to do it, it was rather simple for the following
> comparable systems (WF or SP8). Still time consuming though. The key
> to sleeping well is to make a backup of the system disk before
> starting anything, no surprise here.
>
> Because I had that many systems to upgrade, I made a web page with the
> procedure that I used as reference with the following systems. Since
> it might be useful to some of you, here is the link.
> https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bioimaging.bm
> c.med.uni-2Dmuenchen.de_manuals-2Dprotocols_maintenance-5Fprotocolls_w
> in7towin10_index.html&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8
> rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkb
> ongtDE0&s=5lafTeoK-FTvGr-LVudmzLXQy3JJHEG29aWxam7JMMk&e=
>
>
> This is not a procedure endorsed by Leica, but it worked for us. It
> would be of interest to me if this is actually helpful to anybody, if
> so
>
> I would appreciate a message. (I won't be able to read e-mails from
> this
>
> account next week and the week thereafter, sorry, any reply will be
> delayed).
>
> Thanks to those who helped with valuable tips.
>
> The usual disclaimer applies: No warranties. Enter at your own risk.
> Make a backup first.
>
> Best
>
> Steffen
>
>
>
--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de
12