software to control Olympus IX81 for video microscopy

[Bernhard Kuhn]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear All,

We have an Olympus IX81 with a motorized stage Prior Proscan II, microscopy
control unit IX2-USB and Olympus Laser- Based Autofocus System (IX2-ZDC).

The camera is a Hamamatsu EM-CCD C9100-02, controlled with A3472-07.

We are using this unit for multi-location, multi-time microscopy ~30
locations for ~150 acquisitions per experiment.

What software and operating system do people recommend to control this
setup?

Is there anyone with a similar setup who can make software recommendations?

Thank you and Happy New Year,

Bernhard Kuhn

[John Oreopoulos]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Check out MicroManager when you get a chance.

John Oreopoulos

On 2011-12-28, at 2:19 PM, Bernhard Kuhn <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
>
> We have an Olympus IX81 with a motorized stage Prior Proscan II, microscopy
> control unit IX2-USB and Olympus Laser- Based Autofocus System (IX2-ZDC).
>
> The camera is a Hamamatsu EM-CCD C9100-02, controlled with A3472-07.
>
> We are using this unit for multi-location, multi-time microscopy ~30
> locations for ~150 acquisitions per experiment.
>
> What software and operating system do people recommend to control this
> setup?
>
> Is there anyone with a similar setup who can make software recommendations?
>
> Thank you and Happy New Year,
>
> Bernhard Kuhn

[Gary G. Li-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Bernhard Kuhn,

Discuss with a technical support of Molecular Devices to see if their
MetaMorph fits your requirements.

http://www.moleculardevices.com/

Happy Holidays!

Gary G Li, PhD

Microscopy Facility Manager

NIDA/NIH

Baltimore, MD 21224


On Wed, Dec 28, 2011 at 2:19 PM, Bernhard Kuhn <[hidden email]
> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
>
> We have an Olympus IX81 with a motorized stage Prior Proscan II, microscopy
> control unit IX2-USB and Olympus Laser- Based Autofocus System (IX2-ZDC).
>
> The camera is a Hamamatsu EM-CCD C9100-02, controlled with A3472-07.
>
> We are using this unit for multi-location, multi-time microscopy ~30
> locations for ~150 acquisitions per experiment.
>
> What software and operating system do people recommend to control this
> setup?
>
> Is there anyone with a similar setup who can make software recommendations?
>
> Thank you and Happy New Year,
>
> Bernhard Kuhn
>

--
Gary G. Li, Ph.D.

[mahogny]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

> Check out MicroManager when you get a chance.
>

I concur. that is where things are heading, and your only bet if you want
to squeeze out everything your system can do

I always recommend linux as otherwise everything seems to be about handling
your virus infected windows computer. unfortunately I don't think your
hardware is supported on this OS yet (please put some pressure on olympus)

/Johan

>
> John Oreopoulos
>
> On 2011-12-28, at 2:19 PM, Bernhard Kuhn <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear All,
> >
> > We have an Olympus IX81 with a motorized stage Prior Proscan II,
> microscopy
> > control unit IX2-USB and Olympus Laser- Based Autofocus System (IX2-ZDC).
> >
> > The camera is a Hamamatsu EM-CCD C9100-02, controlled with A3472-07.
> >
> > We are using this unit for multi-location, multi-time microscopy ~30
> > locations for ~150 acquisitions per experiment.
> >
> > What software and operating system do people recommend to control this
> > setup?
> >
> > Is there anyone with a similar setup who can make software
> recommendations?
> >
> > Thank you and Happy New Year,
> >
> > Bernhard Kuhn
>

--
-----------------------------------------------------------
Johan Henriksson
PhD student, Karolinska Institutet
http://mahogny.areta.org  http://www.endrov.net

[Edelmann, Richard E. Dr.]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Bernhard:

  A couple of other options to follow up on.  Image Pro Plus (www.mediacy.com) does directly control the IX-81, The Prior Stages, and has very flexible macro programing controls fro allowing the slide scanning.  I do not know about the Hamamatsu interface.

  Secondly, Slide-Book ( www.intelligent-imaging.com) will also control the IX-81.  We have an older copy running deconvolution on one of our IX-81's.



Richard E. Edelmann, Ph.D.
Center for Advanced Microscopy & Imaging, Director
Miami University
Oxford, OH 45056

Ph: 513.529.5712
www.cami.muohio.edu
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Bernhard Kuhn [[hidden email]]
Sent: Wednesday, December 28, 2011 2:19 PM
To: [hidden email]
Subject: software to control Olympus IX81 for video microscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear All,

We have an Olympus IX81 with a motorized stage Prior Proscan II, microscopy
control unit IX2-USB and Olympus Laser- Based Autofocus System (IX2-ZDC).

The camera is a Hamamatsu EM-CCD C9100-02, controlled with A3472-07.

We are using this unit for multi-location, multi-time microscopy ~30
locations for ~150 acquisitions per experiment.

What software and operating system do people recommend to control this
setup?

Is there anyone with a similar setup who can make software recommendations?

Thank you and Happy New Year,

Bernhard Kuhn

[Paul Rigby-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi All,
I have also been trying to control an IX81 with almost the identical hardware to Bernhard (Prior stage, ZDC etc) using Micromanager. However, I have been unable to get our computer (and Micromanager) to successfully talk with the Olympus control box via the serial port. I suspect the serial port settings. If anyone has done this successfully, can you please let me/us know what settings you used or what has worked best.

Many thanks.
Paul

Assoc. Prof. Paul Rigby
Centre for Microscopy, Characterisation & Analysis (M510)
The University of Western Australia
35 Stirling Highway
Crawley  WA  6007
Australia

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of John Oreopoulos
Sent: Thursday, 29 December 2011 4:37 AM
To: [hidden email]
Subject: Re: software to control Olympus IX81 for video microscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Check out MicroManager when you get a chance.

John Oreopoulos

On 2011-12-28, at 2:19 PM, Bernhard Kuhn <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
>
> We have an Olympus IX81 with a motorized stage Prior Proscan II, microscopy
> control unit IX2-USB and Olympus Laser- Based Autofocus System (IX2-ZDC).
>
> The camera is a Hamamatsu EM-CCD C9100-02, controlled with A3472-07.
>
> We are using this unit for multi-location, multi-time microscopy ~30
> locations for ~150 acquisitions per experiment.
>
> What software and operating system do people recommend to control this
> setup?
>
> Is there anyone with a similar setup who can make software recommendations?
>
> Thank you and Happy New Year,
>
> Bernhard Kuhn

[mahogny]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I had trouble with the IX81 once and solved it by increasing the timeout
(in the hardware config). does this help?

my timeout setting was probably higher than listed here:
http://valelab.ucsf.edu/~MM/MMwiki/index.php/OlympusIX81/BX61

/Johan

On Thu, Jan 5, 2012 at 4:55 AM, Paul Rigby <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi All,
> I have also been trying to control an IX81 with almost the identical
> hardware to Bernhard (Prior stage, ZDC etc) using Micromanager. However, I
> have been unable to get our computer (and Micromanager) to successfully
> talk with the Olympus control box via the serial port. I suspect the serial
> port settings. If anyone has done this successfully, can you please let
> me/us know what settings you used or what has worked best.
>
> Many thanks.
> Paul
>
> Assoc. Prof. Paul Rigby
> Centre for Microscopy, Characterisation & Analysis (M510)
> The University of Western Australia
> 35 Stirling Highway
> Crawley  WA  6007
> Australia
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of John Oreopoulos
> Sent: Thursday, 29 December 2011 4:37 AM
> To: [hidden email]
> Subject: Re: software to control Olympus IX81 for video microscopy
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Check out MicroManager when you get a chance.
>
> John Oreopoulos
>
> On 2011-12-28, at 2:19 PM, Bernhard Kuhn <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear All,
> >
> > We have an Olympus IX81 with a motorized stage Prior Proscan II,
> microscopy
> > control unit IX2-USB and Olympus Laser- Based Autofocus System (IX2-ZDC).
> >
> > The camera is a Hamamatsu EM-CCD C9100-02, controlled with A3472-07.
> >
> > We are using this unit for multi-location, multi-time microscopy ~30
> > locations for ~150 acquisitions per experiment.
> >
> > What software and operating system do people recommend to control this
> > setup?
> >
> > Is there anyone with a similar setup who can make software
> recommendations?
> >
> > Thank you and Happy New Year,
> >
> > Bernhard Kuhn
>

--
-----------------------------------------------------------
Johan Henriksson
PhD student, Karolinska Institutet
http://mahogny.areta.org  http://www.endrov.net

[tineke vendrig]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Daer all,

We are working with Andor software and this will work fine, after having
some additional back-up/support.

Best,

Tineke Vendrig

technical engineer optical microscopy

Delft University of Technology

Bionano Science

Kavli Institute of Nanoscience

Lorentzweg 1

2628LJ Delft

room F185

Tel: [phone]

Fax:[phone]

email: [hidden email]

mobile phone: [phone]

2012/1/5 Paul Rigby <[hidden email]>

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi All,
> I have also been trying to control an IX81 with almost the identical
> hardware to Bernhard (Prior stage, ZDC etc) using Micromanager. However, I
> have been unable to get our computer (and Micromanager) to successfully
> talk with the Olympus control box via the serial port. I suspect the serial
> port settings. If anyone has done this successfully, can you please let
> me/us know what settings you used or what has worked best.
>
> Many thanks.
> Paul
>
> Assoc. Prof. Paul Rigby
> Centre for Microscopy, Characterisation & Analysis (M510)
> The University of Western Australia
> 35 Stirling Highway
> Crawley  WA  6007
> Australia
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of John Oreopoulos
> Sent: Thursday, 29 December 2011 4:37 AM
> To: [hidden email]
> Subject: Re: software to control Olympus IX81 for video microscopy
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Check out MicroManager when you get a chance.
>
> John Oreopoulos
>
> On 2011-12-28, at 2:19 PM, Bernhard Kuhn <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear All,
> >
> > We have an Olympus IX81 with a motorized stage Prior Proscan II,
> microscopy
> > control unit IX2-USB and Olympus Laser- Based Autofocus System (IX2-ZDC).
> >
> > The camera is a Hamamatsu EM-CCD C9100-02, controlled with A3472-07.
> >
> > We are using this unit for multi-location, multi-time microscopy ~30
> > locations for ~150 acquisitions per experiment.
> >
> > What software and operating system do people recommend to control this
> > setup?
> >
> > Is there anyone with a similar setup who can make software
> recommendations?
> >
> > Thank you and Happy New Year,
> >
> > Bernhard Kuhn
>

[Nico Stuurman]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Paul,

> I have also been trying to control an IX81 with almost the identical hardware to Bernhard (Prior stage, ZDC etc) using Micromanager. However, I have been unable to get our computer (and Micromanager) to successfully talk with the Olympus control box via the serial port. I suspect the serial port settings. If anyone has done this successfully, can you please let me/us know what settings you used or what has worked best.

It can be challenging to get hardware to cooperate!  However, there are many labs that are using Micro-Manager to operate an Olympus IX81.  If you still have trouble after increasing the serial port timeout as suggested by Johan, please use the menu entry "Help"->"Report a Problem".  This will give me a chance to figure out what is going on and help you make it work.  

Also, the Micro-Manager mailing list ( https://lists.sourceforge.net/lists/listinfo/micro-manager-general ) may be a more useful forum for issues such as these.

Best,

Nico